LOCUS       sequence-378889-       10565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteroides - Escherichia coli shuttle vector with TetQ and 
            Kanamycin selection markers BS2 is cloned under the Bacteroides 
            thetaiotaomicron ahpC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Jones KR, Belvin BR, Macrina FL, Lewis JP
  TITLE     Sequence and characterization of shuttle vectors for molecular 
            cloning in Porphyromonas, Bacteroides and related bacteria.
  JOURNAL   Mol Oral Microbiol. 2020 Aug;35(4):181-191. doi: 10.1111/omi.12304. 
            Epub 2020 Jul 9.
  PUBMED    32592236
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/omi.12304";
            journalName: "Mol Oral Microbiol"; date: "2020-08"; volume: "35"; 
            issue: "4"; pages: "181-191"
COMMENT     Sequence Label: pG108-K - AhpC-Glow
FEATURES             Location/Qualifiers
     source          1..10565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     237..256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             5004..5012
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     5419..5436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(6177..6182,6183..6200,6201..6207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6215..6231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6220..6242
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6239..6255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6239..6255
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6251..6574
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    6268..6324
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6325..6342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6325..6341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6334..6356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6550..6569)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(9376..10191)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9376..10191)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10099..10118
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10183..10202
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(10313..10565,1..336)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
       61 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      361 cggttcctgg ccttttgctg gccttttgct cacatgtgat cctctattgc agatggtctt
      421 gttgcacacc cctctgaagg gttctatatc atctatacca cctcttcaga ggagtttctc
      481 acgttatctg aagaagatta ttctctgttc gcttcttttg ccaatttggt tgttcgagag
      541 gactccaatt caaatggctt aagaagcgca aaaccctctg gggatggctg gaaatttgga
      601 ggtaaaggaa aggggaaact agatgccatc cacatcggct atgaattatc gaaaatccta
      661 ggagaaaatc aagattttga gatctatgta gaatacaatg gcgatgggtc ttataccgtt
      721 tggtataggg aagtccaaaa atcgtctaaa gggagtaatc cgtaaagact tttaatgtcc
      781 tcataagagc caacgaaaga gaggggcata cgtctctctc tttcaacgta tgcttgaacg
      841 attgcttact cgatgtgatt aaatagagct ggcttgatag gtcagctcta tttctgtttt
      901 tcgttttgaa aatatgatag gcaaatacca tggatatgac ataacagaaa tagagctact
      961 aagcccaaag ccacgaacca taacgatgtc agaggatcac gtggcgctac ttagtagctc
     1021 tatctcactt gcaaagttac taaaagagat ctgcgtgcga gcctaagcgt gagaggacga
     1081 taagctcctc gtctgcgctt atctgctcta tccatatcaa gagcaggtca ccctcaatgt
     1141 gtagctccat acagccctca tagttcccta taagcttgtg aggacgcatt tctaagggta
     1201 tctccacacc cttagacagc atttcaatga catctacgat tttagagcgc cttttgaggt
     1261 cgttcctaaa tcgcttaagg tctttcttaa acttggtagt atataccagc ctacgcttcc
     1321 tcatttccaa acaaagacgc ttctattgtt tctcgggtag ttgtatccac agagccggca
     1381 tagcgaccgc tcttagcgtc tatgcaggcc tctgccgtct cggcattata aggtctatac
     1441 cccattcggt agagtaaggt ctcaatatag ttgcttaggc ttctattctc agcccttgct
     1501 tcactgcgta tctgctggag tacactagca tcaattctaa ttgacgtgga tgcttttagc
     1561 tcaatattat ccatttctat tatattctac ttgattatat tcactgcaaa tatagacaaa
     1621 tctcccaaat aaacgtctgt cggctgggcg tcagttcacg caccaaatgt gtgtgtaatg
     1681 ggagcgacaa gaagttgtcc aataaaatcg ttctccgtaa ctttgggcag gcgctagcta
     1741 cgaccatagt tatagcacat tataactaca tacgtagttg tgtgctctcc gaggggcatg
     1801 gaggacaccc ccagcacccc ctaaagttga cccaaaagta caagcgatga gcaaaacaag
     1861 tatccatatc gagccgtgta aaatcggcag tagtgagcaa cataatcaac gcttgaagca
     1921 tcttgattat gtgcgacccg acctctcccc acaaaacgag agttgggttg gcgtagcaga
     1981 tttgcccgcg catcttgaac aactccgaat acttgtaaag gagaagacgg gcagagcgat
     2041 gcaacagaaa gcaaccccga taagagaggg tgtaatagta atcaatcaag gcacctcgat
     2101 taaccaatta aggggtctcg caacggcaat agaactgcga tggggtatta agacgctaca
     2161 gatatacacc cacaaggacg aggggcatat agatagcgat gacacgtgga agccaaacct
     2221 acatgcccat atggtgcttg attgggtgaa ccacgacacg ggcaagagta tcaagcttag
     2281 caagcaagat atggcggaga tgcagacgat ggtggcggac tgcctgcaaa tggttagggg
     2341 agagagtagc gatgtcaaac acctcggggc tatacaatac aagaatcaag ccgaagagct
     2401 gcgtctccaa acccttaaag aacaaacgat gaaagaagaa caggctcggg agctggcact
     2461 agccgaagct aagcggatgg agcaggttgc gtctgagcag attatcgcag agaataagaa
     2521 gcttggaggg ctgtggatgg actggcaaaa atcatttgag gcctctgaga agcgccgaga
     2581 gagcataacg gctcgtaaga tagaggagga taagctatgg gaagaacttg ggttgaaagg
     2641 agcctttaaa tcgttttggg aggaatgcaa ggattggttt agaggtttcg gcgatgggct
     2701 caaagactta gctctgctga tatgggcttc gcctagagga gtgatggaca aagacgtgag
     2761 gtgcacagcg gatcggaaga gggggaaatt actgctcaat gggcatacac tagagcaggt
     2821 aagagcaccc aagatagttg taccaagctc ccccatacaa aggcaggcgc aatccgtcag
     2881 tgagtgcgct acgaggcagg ctagagatac agacagacgc agagagaagc gtgttgacca
     2941 agagagacga gagcgcaggg gtcgcaagat ttgaccgcac tatgttctta aagcgtttcc
     3001 caagggcttt gccaccactt gcggacggtc ggtctactta gtcctgtctc tctcgcacaa
     3061 cggctcttgt tgttgctgtt tgggtttcta tccctccact cctgcacctt ggcgtattgc
     3121 gggctgtctt ccgctagtac gatagagcct tttggtcttc cgttaccctc tctccaatct
     3181 tttcttccct ttgcttcctg caaaatatct ctagagcctc ttgcgagctt tagatgtaag
     3241 tcttgcggtc tcccattgcg tttgttaggt tctattctta gccccgacct atcctctatc
     3301 agctggcgag ggtatgtctc tgagctctcc ttatatgctt ttagagcgtc tagagcgtcc
     3361 tgcacggaga agtgcctgcc cgtgttgccc gttagtgctt ccattcgtgg cacaagctct
     3421 agagcgtccc gtttgagtat gtcaaatggc acgttacact tcttggcata tactgctaag
     3481 aacataatgc agtggtagcg atgcccaaca gcgacctttt cgttcgtcca cagcatatgt
     3541 agccaccact catacagccc cctatgcaca tgccacgtct tacgaggtgc gccctctacg
     3601 atgcgttttt gataccaatc tgggtatagc tctttagctc ttgcttttgt tagcctattc
     3661 gtggagtatg cccctttgtc caacgcttgc gcctgctcct gcgacacccc cgagttaagg
     3721 tacttcagtg taggcttgct agcaaaccga ttcagctcct caatagtgtg tagtggggca
     3781 tcagcattgt gccaagctgt tacaatgtcc ccgtgcttcg tacgactgcc tggcacccta
     3841 aacggctgta taatgggctg tatttgcact tggttgtcct cccccgtcct agaggacgct
     3901 ttgttccata taagccgtgt aagagctttt ttaatgttgg tgagcgtctc gtacaccttg
     3961 gggtataagg gcatcggctt agcaagtatg tagtacaagt ggatgccatc gcccgagttg
     4021 acgatgatat tagcttgtgg ggtcatccct atcgtctgct gatggattac ctctgacatc
     4081 tctggcactc cgacctcatc aaggtcaaag gcgagggcat acagatagcg ggcattgctc
     4141 ttgaacctcc tgcgccccac gtaggtggct ggggttgtta ccacaaactt gtaccgcccc
     4201 atcagctcct ctaagctatc ccaaccacac accttgatgt catcctcgct tgtggcatcg
     4261 cccactacca cccacgtctt agacctcctg ccattatcat tagccctgcc tgcatcaggc
     4321 tctacgctgt agacagcttg caccaaagca ttatagcgga aaggttgagc tggattatag
     4381 attgctttct tacctgcttt ggacttagac tttctccctg cttgttgagc cctccaatcg
     4441 atgtattctt ggcacagctc tttttcaaaa ggatagatag ggcattgcgg gtcattatgg
     4501 tcacaagaac caaagattat ttggaagaac tcaaagggtt taagagatcg atattcaaag
     4561 gtctcattac tctctaagta taaattttta gagagaaaaa catcgtgaga aatattagaa
     4621 ttatcagtca tcgttctacg tcattttggg gtgatttttt ggtgtcgctt cgctccccca
     4681 aaaaatcata cggggggcat atattatcaa tatatttttt ttgccacttg tggcacaaag
     4741 ataaggagga tttttgagac ctccttctac gtcattttgg ggtgattttt tggtgtcgct
     4801 tcgctccccc aaaaaatcat acggggggca tatattatca atatattttt ttttgccact
     4861 tgtggcacaa agataaggag gatttttgag acctccttct acgtcatttt gggtggaatt
     4921 tttggctcgc tacgctcccc aaaaattcat acgggggggc gtatattatc aatgaagtaa
     4981 aatccccact tgtggtacaa aggtaaggag gttttttgag acctccttct gcgtcatcgt
     5041 tctacgtcat tttggggttg attttttggg gaagaaacaa agaatatcgc aacaagtagg
     5101 tattgcatat attttttgca attctttatc tttgcggtaa gacaacctct taagagagac
     5161 ttatcttgaa tacaaaacac taaacctaag cagctatgaa aactagaaca ttctgcgcaa
     5221 tagcaatcgg gctattgtct ttggcgttaa cttcgtgcca aaacaacctt gacttgttaa
     5281 agccaagcca agacactgaa ccagaacaaa tcactcaatt acatgttctt tcctgcgtta
     5341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcgagcagct
     5581 tttcatattc cttctgcttg gtgatgtcgt tctggatgcc gacgaaatac gtcttatcct
     5641 cgatttccat cggatcgatg ttcagttcgt tccagaacat cgtgccgtcc ttcttgtagt
     5701 tctggatctg cacggtgacc ggttctttat tttgcagcgc ggtgcggatg ttgtccactt
     5761 ccgccggatc ggtgtgcttc ccctggagga agcgcgcgtt cttgcccagg atttcctcgg
     5821 tctcgtagcc ggtcatctgc acgaagccct ggttcacgta gacgatcggg ttatcttcca
     5881 gcgcgggatc ggtgatgacc acgccgacgc gcacgtgatc cagcgccttc ttgatgactt
     5941 ccagctggcc cgggatgccg aacgactgga acgacgccat catactcttt attgctttta
     6001 ttgttattaa ttgatttgac ttataacatc agaaacatcg ttttgttttt tgtttctgat
     6061 gcaaagataa aggcggaaac cccgcctttg caaaaaaagt ccaatcgaat aattctatcg
     6121 gataatagat gaaaccaata tgcctgccag tgaagctgcc ttcttgcacc ccaggcttta
     6181 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6241 ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga
     6301 tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6361 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6421 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6481 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     6541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     6601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     6661 gaccgtctcc gggagtccat cactcctcta ggcgaagccc atatcagcag agctaagacg
     6721 tcaccagttg aacctacgtt tccctaataa aatgtctatg gtaaaaagtt ataaaatcct
     6781 cctacttttg ttagatatat ttttttgtgt aattttgtaa tcgttatgcg gcagtaataa
     6841 tatacatatt aatacgagtt aggaatcctg tagttctcat atgctacgag gaggtattaa
     6901 aaggtgcgtt tcgacaatgc atctattgta gtatattatt gcttaatcca aatgaatatt
     6961 ataaatttag gaattcttgc tcacattgat gcaggaaaaa cttccgtaac cgagaatctg
     7021 ctgtttgcca gtggagcaac ggaaaagtgc ggctgtgtgg ataatggtga caccataacg
     7081 gactctatgg atatagagaa acgtagagga attactgttc gggcttctac gacatctatt
     7141 atctggaatg gtgtgaaatg caatatcatt gacactccgg gacacatgga ttttattgcg
     7201 gaagtggagc ggacattcaa aatgcttgat ggagcagtcc tcatcttatc cgcaaaggaa
     7261 ggcatacaag cgcagacaaa gttgctgttc aatactttac agaagctgca aatcccgaca
     7321 attatattta tcaataagat tgaccgagcc ggtgtgaatt tggagcgttt gtatctggat
     7381 ataaaagcaa atctgtctca agatgtcctg tttatgcaaa atgttgtcga tggatcggtt
     7441 tatccggttt gctcccaaac atatataaag gaagaataca aagaatttgt atgcaaccat
     7501 gacgacaata tattagaacg atatttggcg gatagcgaaa tttcaccggc tgattattgg
     7561 aatacgataa tcgctcttgt ggcaaaagcc aaagtctatc cggtgctaca tggatcagca
     7621 atgttcaata tcggtatcaa tgagtcgttg gacgccatca cttcttttat acttcctccg
     7681 gcatcggtct caaacagact ttcgtcttat ctttataaga tagagcatga ccccaaagga
     7741 cataaaagaa gttttctaaa aataattgac ggaagtctga gacttcgaga cgttgtaaga
     7801 atcaacgatt cggaaaaatt catcaagatt aaaaatctaa aaactatcaa tcagggcaga
     7861 gagataaatg ttgatgaagt gggcgccaat gatatcgcga ttgtagagga tatggatgat
     7921 tttcgaatcg gaaattattt aggtgctgaa ccttgtttga ttcaaggatt atcgcatcag
     7981 catcccgctc tcaaatcctc cgtccggcca gacaggcccg aagagagaag caaggtgata
     8041 tccgctctga atacattgtg gattgaagac ccgtctttgt ccttttccat aaactcatat
     8101 agtgatgaat tggaaatctc gttatatggt ttaacccaaa aggaaatcat acagacattg
     8161 ctggaagaac gattttccgt aaaggtccat tttgatgaga tcaagactat atacaaagaa
     8221 cgacctgtaa aaaaggtcaa taagattatt cagatcgaag tgccgcccaa cccttattgg
     8281 gccacaatag ggctgactct tgaaccctta ccgttaggga cagggttgca aatcgaaagt
     8341 gacatctcct atggttatct gaaccattct tttcaaaatg ccgtttttga agggattcgt
     8401 atgtcttgcc aatccgggtt acatggatgg gaagtgactg atctgaaagt aacttttact
     8461 caagccgagt attatagccc ggtaagtaca cctgctgatt tcagacagct gaccccttat
     8521 gtcttcaggc tggccttgca acagtcaggt gtggacattc tcgaaccgat gctctatttt
     8581 gagttgcaga taccccaagc ggcaagttcc aaagctatta cagatttgca aaaaatgatg
     8641 tctgagattg aagacatcag ttgcaataat gagtggtgtc atattaaagg gaaagttcca
     8701 ttaaatacaa gtaaagacta tgcatcagaa gtaagttcat acactaaggg cttaggcatt
     8761 tttatggtta agccatgcgg gtatcaaata acaaaaggcg gttattctga taatatccgc
     8821 atgaacgaaa aagataaact tttattcatg ttccaaaaat caatgtcatc aaaataatgg
     8881 agcggtcagg aaatttctat aaggcaatac agttgggata tatacttatc tccattctta
     8941 tcggatgtat ggcatataat agcctctatg aatggcagga gagacgtcag gtggcacttt
     9001 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9061 tccgctcatg agacaataac cctgataaat gcttcaataa taattcgagc tcggtacccc
     9121 cgatagcttc cgctattgct tttttgctca tcggtatttg caacatcata gaaattgcat
     9181 acctttgttc ctcggttata tgtttgctca tctgcaactt ttttttcttt ggacggacaa
     9241 ttaaagcaaa gatagcaaac tttatccatt cagagtgaga gaaaggggga cattgtctct
     9301 ctttcaaatg ctggaagttg tccctgaaaa atttcatcct tcgtagttca aagtcgggtg
     9361 gttgtcaaga tgttcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     9421 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     9481 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     9541 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     9601 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     9661 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     9721 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     9781 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     9841 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     9901 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     9961 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
    10021 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
    10081 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
    10141 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct
    10201 tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg
    10261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10561 gcgat
//
