LOCUS       sequence-378924-        5805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5805)
  TITLE     Oka Lab Plasmids 2022
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: scAV0.1-TBG-mC
FEATURES             Location/Qualifiers
     source          1..5805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      110..623
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(197..216)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     407..428
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        993..1402
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin 
                     gene (Yan et al., 2012)"
     regulatory      1439..1448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1445..2155
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1590..1608)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1858..1877
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2228..2248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(2436..2453)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2442..2649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2662..2791
                     /label=AAV2 ITR
     primer_bind     3438..3460
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3498..3516)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3584..3688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3689..3757,3758..4549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3907..3926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4720..5308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5209..5228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg
       61 attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta cgcgccctgt
      121 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      181 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      241 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      301 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      361 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      421 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
      481 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
      541 aacaaaatat taacgcttac aatttaaata tttgcttata caatcttcct gtttttgggg
      601 cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc
      661 atcgccctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
      721 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggaattcacg
      781 cgtggatctg aattcaattc acgcgtggta cgtggagtcg tgacgtgaat tacgtcatag
      841 ggttagggag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt gcgacaccat
      901 gtggtcacgc tgggtattta agcccgagtg agcacgcagg gtctccattg ctaggggctg
      961 gaagctacct ttgacatcat ttcctctgcg aatgcatgta taatttctac agaacctatt
     1021 agaaaggatc acccagcctc tgcttttgta caactttccc ttaaaaaact gccaattcca
     1081 ctgctgtttg gcccaatagt gagaactttt tcctgctgcc tcttggtgct tttgcctatg
     1141 gcccctattc tgcctgctga agacactctt gccagcatgg acttaaaccc ctccagctct
     1201 gacaatcctc tttctctttt gttttacatg aagggtctgg cagccaaagc aatcactcaa
     1261 agttcaaacc ttatcatttt ttgctttgtt cctcttggcc ttggttttgt acatcagctt
     1321 tgaaaatacc atcccagggt taatgctggg gttaatttat aactaagagt gctctagttt
     1381 tgcaatacag gacatgctat aaaaatggaa agataccggt gctagcaagc ttggatccgc
     1441 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     1501 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     1561 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     1621 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     1681 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     1741 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     1801 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     1861 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     1921 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     1981 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2041 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2101 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagcggc
     2161 cgctcgaggt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2221 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2281 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     2341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2401 gcactgacaa ttccgtggtg gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     2461 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2521 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2581 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2641 tgctggggag agatcgatct gaggaacccc tagtgatgga gttggccact ccctctctgc
     2701 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     2761 gggcggcctc agtgagcgag cgagcgcgca gccccccccc cccccggcga ttctcttgtt
     2821 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
     2881 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     2941 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     3001 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     3061 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     3121 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
     3181 tcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     3241 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3301 actatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3361 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3421 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3481 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3541 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4861 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5101 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5461 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5521 gcgttggccg attcattaat gcagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5581 ttcccaacag ttgcgcagcc tgaatggcga atggcgattc cgttgcaatg gctggcggta
     5641 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
     5701 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
     5761 ttttactcgg tggcctcact gattataaaa acacttctca ggatt
//
