LOCUS       sequence-378925-        6248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  TITLE     Oka Lab Plasmids 2022
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: scAV0.1-TBG-iCre
FEATURES             Location/Qualifiers
     source          1..6248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        327..736
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin 
                     gene (Yan et al., 2012)"
     CDS             782..802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1904..1924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(2112..2129)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2118..2325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2338..2467
                     /label=AAV2 ITR
     primer_bind     3215..3237
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3275..3293)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3361..3465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3466..3534,3535..4326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3684..3703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4497..5085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4986..5005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5692..6205
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5779..5798)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5989..6010
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggaatt cacgcgtgga
      121 tctgaattca attcacgcgt ggtacgtgga gtcgtgacgt gaattacgtc atagggttag
      181 ggaggtcctg tattagaggt cacgtgagtg ttttgcgaca ttttgcgaca ccatgtggtc
      241 acgctgggta tttaagcccg agtgagcacg cagggtctcc attgctaggg gctggaagct
      301 acctttgaca tcatttcctc tgcgaatgca tgtataattt ctacagaacc tattagaaag
      361 gatcacccag cctctgcttt tgtacaactt tcccttaaaa aactgccaat tccactgctg
      421 tttggcccaa tagtgagaac tttttcctgc tgcctcttgg tgcttttgcc tatggcccct
      481 attctgcctg ctgaagacac tcttgccagc atggacttaa acccctccag ctctgacaat
      541 cctctttctc ttttgtttta catgaagggt ctggcagcca aagcaatcac tcaaagttca
      601 aaccttatca ttttttgctt tgttcctctt ggccttggtt ttgtacatca gctttgaaaa
      661 taccatccca gggttaatgc tggggttaat ttataactaa gagtgctcta gttttgcaat
      721 acaggacatg ctataaaaat ggaaagatac cggtgctagc aagcttggat ccaccatggt
      781 gcccaagaag aagaggaaag tctccaacct gctgactgtg caccaaaacc tgcctgccct
      841 ccctgtggat gccacctctg atgaagtcag gaagaacctg atggacatgt tcagggacag
      901 gcaggccttc tctgaacaca cctggaagat gctcctgtct gtgtgcagat cctgggctgc
      961 ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa cctgaggatg tgagggacta
     1021 cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc atccaacagc acctgggcca
     1081 gctcaacatg ctgcacagga gatctggcct gcctcgccct tctgactcca atgctgtgtc
     1141 cctggtgatg aggagaatca gaaaggagaa tgtggatgct ggggagagag ccaagcaggc
     1201 cctggccttt gaacgcactg actttgacca agtcagatcc ctgatggaga actctgacag
     1261 atgccaggac atcaggaacc tggccttcct gggcattgcc tacaacaccc tgctgcgcat
     1321 tgccgaaatt gccagaatca gagtgaagga catctcccgc accgatggtg ggagaatgct
     1381 gatccacatt ggcaggacca agaccctggt gtccacagct ggtgtggaga aggccctgtc
     1441 cctgggggtt accaagctgg tggagagatg gatctctgtg tctggtgtgg ctgatgaccc
     1501 caacaactac ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt ctgccacctc
     1561 ccaactgtcc acccgggccc tggaagggat ctttgaggcc acccaccgcc tgatctatgg
     1621 tgccaaggat gactctgggc agagatacct ggcctggtct ggccactctg ccagagtggg
     1681 tgctgccagg gacatggcca gggctggtgt gtccatccct gaaatcatgc aggctggtgg
     1741 ctggaccaat gtgaacattg tgatgaacta catcagaaac ctggactctg agactggggc
     1801 catggtgagg ctgctcgagg atggggactg agcggccgct cgaggtcgac aatcaacctc
     1861 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1921 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     1981 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
     2041 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtggagc
     2101 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2161 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2221 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2281 cagcaagggg gaggattggg aagacaatag caggcatgct ggggagagat cgatctgagg
     2341 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2401 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     2461 cgcgcagaga gggagngggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2521 aggcatgctg gggagagatc gatctgagga acccctagtg atggagttgg ccactccctc
     2581 tctgcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga
     2641 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca
     2701 tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca
     2761 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga
     2821 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt
     2881 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga
     2941 tttattggat gttggaatcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3001 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3061 ccccgacacc cgccaacact atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3121 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3181 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3241 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     3301 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3361 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3421 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3481 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3541 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3601 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3661 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3721 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3781 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3841 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3901 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3961 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4021 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4081 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4141 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4201 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4261 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4321 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4381 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4621 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5281 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcgtaa tagcgaagag
     5341 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgattccgt
     5401 tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc
     5461 ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt
     5521 gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga
     5581 ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc
     5641 tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct
     5701 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5761 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5821 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5881 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5941 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6001 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6061 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6121 ttaacaaaat attaacgctt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
     6181 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt
     6241 tcatcgcc
//
