LOCUS       sequence-379019-       10864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for stable integration of inducible, 
            siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation)
            with C-terminal mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Paine EL, Skalicky JJ, Whitby FG, Mackay DR, Ullman KS, Hill CP, 
            Sundquist WI
  TITLE     The Calpain-7 protease functions together with the ESCRT-III protein
            IST1 within the midbody to regulate the timing and completion of 
            abscission.
  JOURNAL   Elife. 2023 Sep 29;12:e84515. doi: 10.7554/eLife.84515.
  PUBMED    37772788
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-09-29"; volume: "12:e84515. doi"; pages: " 
            10.7554/eLife.84515"
COMMENT     Sequence Label: pLVX-CAPN7-C290S-mCherry
FEATURES             Location/Qualifiers
     source          1..10864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2205..2519
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2284..2302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2320..2338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2492..2516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2533..2542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4978..5685
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5120..5138)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5388..5407
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5696..6195
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5731..5754)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6128..6147
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6216..6815
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6216..6235)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6712..6732
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6832..7420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6885..6905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7303..7314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7627..8260
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8388..8404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8388..8404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8401..8423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8412..8428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8436..8442,8443..8460,8461..8466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8481..8502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8619..8636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8790..9378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8870..8889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9549..10340,10341..10409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10172..10191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10410..10514)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10562..10696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10599..10618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10653..10672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10864,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
     2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2521 ggagaaggat ccgccaccat ggacgccaca gcactggagc gggacgctgt gcagttcgcc
     2581 cgtctggcgg ttcagcgcga ccacgaaggc cgctactccg aggcggtgtt ttattacaag
     2641 gaagctgcac aagccttaat ttatgctgag atggcaggat caagcctaga aaatattcaa
     2701 gaaaaaataa ctgagtatct ggaaagagtt caagctctac attcagcagt tcagtcaaag
     2761 agtgctgatc ctttgaagtc aaaacatcag ttggacttag agcgtgctca tttccttgtt
     2821 acacaagctt ttgatgaaga tgaaaaagag aatgttgaag atgctataga attgtacaca
     2881 gaagctgtgg atctctgtct gaaaacatct tatgaaactg ctgataaagt cctgcaaaat
     2941 aaactgaaac agttggctcg acaggcgcta gacagagcag aagcgctgag tgagcctttg
     3001 accaagccag ttggcaaaat cagttcaaca agtgttaagc caaagccacc tccagtgaga
     3061 gcacattttc cactgggcgc taatcccttc cttgaaagac ctcagtcatt tataagtcct
     3121 cagtcatgtg atgcacaagg acagagatac acagcagaag aaatagaagt actcaggaca
     3181 acatcaaaaa taaatggtat agaatatgtt cctttcatga atgttgacct gagagaacgt
     3241 tttgcctatc caatgccttt ctgtgataga tggggcaagc taccattatc acctaaacaa
     3301 aaaactacat tttccaagtg ggtacgacca gaagacctca ccaacaatcc tacaatgata
     3361 tatactgtgt ccagttttag cataaagcag acaatagtat cggattcctc ctttgtggca
     3421 tcactggcca tcagtgcagc ttatgaaaga cgttttaata agaagttaat taccggcata
     3481 atttaccctc aaaacaagga tggtgaacca gaatacaatc catgtgggaa gtatatggta
     3541 aaacttcacc tcaatggtgt cccaagaaag gtgataattg atgaccagtt acctgttgat
     3601 cacaagggag aattgctctg ttcttattcc aacaacaaaa gtgaattatg ggtttctctc
     3661 atagaaaaag catacatgaa agtcatggga ggatatgatt ttccaggatc caactccaat
     3721 attgatcttc atgcactgac tggctggata ccagaaagaa ttgctatgca ttcagatagc
     3781 caaactttca gtaaggataa ttctttcaga atgctttatc aaagatttca caaaggagat
     3841 gtcctcatca ctgcgtcaac tggaatgatg acagaagctg aaggagagaa gtggggtctg
     3901 gttcccacac acgcatatgc tgttttggat attagagagt tcaaggggct gcgatttatc
     3961 cagttgaaaa atccttggag tcatttacgt tggaaaggaa gatacagtga aaatgatgta
     4021 aaaaactgga ctccagagtt gcaaaagtat ttaaactttg atccccgaac agctcagaaa
     4081 atagacaacg gaatattttg gatttcctgg gatgatctct gccagtatta tgatgtgatt
     4141 tatttgagtt ggaatccagg tctttttaaa gaatcaacat gtattcacag tacttgggat
     4201 gctaagcaag gacctgtgaa agatgcctat agcctggcca acaaccccca gtacaaactg
     4261 gaggtgcagt gtccacaggg gggtgctgca gtttgggttt tgcttagtag acacataaca
     4321 gacaaggatg attttgcgaa taatcgagaa tttatcacaa tggttgtata caagactgat
     4381 gggaaaaaag tttattaccc agctgaccca cctccataca ttgatggaat tcgaattaac
     4441 agccctcatt atttgactaa gataaagctg accacacctg ggacgcacac gttcacgcta
     4501 gtggtttctc aatatgaaaa acagaacaca atccattaca cggttcgggt atattcagca
     4561 tgcagcttta ctttttcaaa gattccttca ccatacacct tatcaaaacg gattaatgga
     4621 aagtggagtg gtcagagtgc tggaggatgt ggaaatttcc aagagactca caaaaataac
     4681 cccatctacc aattccatat agaaaagact gggccgttac tgattgagct acgaggacca
     4741 aggcaatata gcgttggatt tgaggttgta acagtttcta ctctaggaga tcctggtccc
     4801 catggctttc tgaggaaatc tagtggtgac tataggtgtg ggttttgcta cctggaatta
     4861 gaaaatatac cttctgggat cttcaatatc attcctagta cctttttgcc taaacaagaa
     4921 ggaccttttt tcttggactt taatagtatt atccccatca agatcacaca acttcaggtg
     4981 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     5041 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     5101 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     5161 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     5221 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     5281 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     5341 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     5401 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     5461 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     5521 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     5581 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     5641 gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagaatt ctaccgggta
     5701 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     5761 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     5821 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     5881 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     5941 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     6001 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     6061 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     6121 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     6181 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     6241 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6301 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6361 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6421 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6481 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6541 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6601 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6661 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6721 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6781 gcacctggtg catgacccgc aagcccggtg cctgacgggc gcgtctggaa caatcaacct
     6841 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6901 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6961 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7021 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7081 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7141 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7201 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     7261 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7321 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7381 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc
     7441 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7501 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     7621 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7681 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     7741 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     7801 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     7861 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     7921 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     7981 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     8041 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     8101 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8161 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8221 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     8281 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     8341 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10561 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10621 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10681 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10741 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    10801 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    10861 agtt
//
