LOCUS       sequence-379088-        5849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ASAP3ER under control of human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Campbell EP, Abushawish AA, Valdez LA, Bell MK, Haryono M, Rangamani
            P, Bloodgood BL
  TITLE     Electrical signals in the ER are cell type and stimulus specific 
            with extreme spatial compartmentalization in neurons.
  JOURNAL   Cell Rep. 2023 Jan 31;42(1):111943. doi: 
            10.1016/j.celrep.2022.111943. Epub 2023 Jan 5.
  PUBMED    36640310
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-31"; volume: "42(1):111943. doi"; pages: " 
            10.1016/j.celrep.2022.111943. Epub 2023 Jan 5"
COMMENT     Sequence Label: AAV-hSyn-ASAP3ER
FEATURES             Location/Qualifiers
     source          1..5849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        186..633
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1976..2564
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2029..2049)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2447..2458)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2596..3072
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2795..2814)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3112..3252
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3112..3241
                     /label=AAV2 ITR
     rep_origin      3327..3782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3414..3433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3624..3645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3799..3818)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3918..3940
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3978..3996)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4064..4168
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4169..4237,4238..5029)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4387..4406)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5200..5788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5689..5708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cttctagagt
      661 cgacgccacc atggagacga ctgtgaggta tgaacagggg tcagagctca ctaaaacttc
      721 gagctctcca acagcagatg agcccacgat aaagattgat gatggtcgtg atgagggtaa
      781 tgaacaagac agctgttcca ataccattag gagaaaaatt tccccgtttg tgatgtcatt
      841 tggattcaga gtatttggag ttgtgcttat cattgtagac atcatagtgg tgattgtgga
      901 tctggccatc agtgagaaga aaagaggcat tagagagatt cttgaaggtg tttccctggc
      961 tatagcactc ttcttccttg ttgatgttct catgagagtg tttgttgaag gcttcaagaa
     1021 ctatttccgg tccaaactga atactttgga tgcagtcata gtagtgggca ctctgctaat
     1081 taatatgacc tactccttct ctgacggtac atttcgcggt gataacgtgt atattaccgc
     1141 ggataaacag aaaaacggca ttaaagcgaa ctttaccgtg cgccataacg tggaagatgg
     1201 cagcgtgcag ctggcggatc attatcagca gaacaccccg attggcgatg gcccggtgct
     1261 gctgccggat aaccattatc tgagcaccca gaccgtgctg agcaaagatc cgaacgaaaa
     1321 acgcgatcac atggtgctgc tggaatttgt gaccgcagcg ggcattacac acggcatgga
     1381 tgaactgtat ggcggcaccg gcggcagcgc gagccagggc gaagaactgt ttaccggcgt
     1441 ggtgccgatt ctggtggaac tggatggcga tgtgaacggc cataaattta gcgtgcgcgg
     1501 cgaaggcgaa ggcgatgcga ccattggcaa actgaccctg aaatttattt gcaccaccgg
     1561 caaactaccg gtgccgtggc cgaccctggt gaccacctta acctatggcg tgcagtgctt
     1621 tagccgctat ccggatcata tgaaacgcca tgattttttt aaaagcgcga tgccggaagg
     1681 ctatgtgcag gaacgcacca ttagctttaa agatgatggc aaatataaaa cccgcgcggt
     1741 ggtgaaattt gaaggcgata ccctggtgaa ccgcattgaa ctgaaaggca ccgattttaa
     1801 agaagatggc aacattctgg ggcataaact ggaatataac acagatcaga tgccgcagat
     1861 ggttactctt cttcgagttc tgagaattgt tatcttaata agaatatttc agctggcttc
     1921 acagaagaaa caacttgaag tggtaaccaa gaaggcggcg taaaagctta tcgataatca
     1981 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2041 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2101 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2161 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2221 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2281 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2341 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2401 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2461 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2521 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct
     2581 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     2641 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     2701 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     2761 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     2821 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     2881 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     2941 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     3001 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3061 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
     3121 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     3181 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     3241 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3301 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     3361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3481 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3541 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3661 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3721 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3781 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3841 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3901 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3961 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4021 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4081 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4141 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4201 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4261 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4321 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4381 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4441 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4501 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4561 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4621 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4681 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4741 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4801 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4861 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4921 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4981 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5041 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5101 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5161 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5221 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5281 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5341 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5401 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5461 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5521 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5581 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5641 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5701 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5761 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5821 ggccttttgc tggccttttg ctcacatgt
//
