LOCUS       sequence-379128-       11597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations 
            to block Akt phosphorylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Zhong Y, Zhou X, Guan KL, Zhang J
  TITLE     Rheb regulates nuclear mTORC1 activity independent of farnesylation.
  JOURNAL   Cell Chem Biol. 2022 Jun 16;29(6):1037-1045.e4. doi: 
            10.1016/j.chembiol.2022.02.006. Epub 2022 Mar 15.
  PUBMED    35294906
REFERENCE   2  (bases 1 to 11597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.02.006"; journalName: "Cell Chem Biol"; 
            date: "2022-06-16- 16"; volume: "29"; issue: "6"; pages: 
            "1037-1045.e4"
COMMENT     Sequence Label: pcDNA3-mCherry-TSC2(AA)
FEATURES             Location/Qualifiers
     source          1..11597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(979..987,988..1002)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1009..1716
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1154..1172)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1422..1441
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(7150..7168)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7151..7168)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7188..7205)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7194..7418
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7464..7892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7551..7570)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7761..7782
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7901..7921)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7906..8235
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8086..8221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8148..8167
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8302..9096
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8356..8375)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8966..8985
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9270..9391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9307..9326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9361..9380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9440..9456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9440..9456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9453..9475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9464..9480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9488..9494,9495..9512,9513..9518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9533..9554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9671..9688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9842..10430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9922..9941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10601..11392,11393..11461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11224..11243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11462..11566)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1081 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1141 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1201 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1261 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1321 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1381 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1441 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1501 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1561 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1621 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1681 cgccactcca ccggcggcat ggacgagctg tacaagcgca tgcatgcaag ggatccagcc
     1741 aaaccaacaa gcaaagattc aggcttgaag gagaagttta agattctgtt gggactggga
     1801 acaccgaggc caaatcccag gtctgcagag ggtaaacaga cggagtttat catcaccgcg
     1861 gaaatactga gagaactgag catggaatgt ggcctcaaca atcgcatccg gatgataggg
     1921 cagatttgtg aagtcgcaaa aaccaagaaa tttgaagagc acgcagtgga agcactctgg
     1981 aaggcggtcg cggatctgtt gcagccggag cggccgctgg aggcccggca cgcggtgctg
     2041 gctctgctga aggccatcgt gcaggggcag ggcgagcgtt tgggggtcct cagagccctc
     2101 ttctttaagg tcatcaagga ttacccttcc aacgaagacc ttcacgaaag gctggaggtt
     2161 ttcaaggccc tcacagacaa tgggagacac atcacctact tggaggaaga gctggctgac
     2221 tttgtcctgc agtggatgga tgttggcttg tcctcggaat tccttctggt gctggtgaac
     2281 ttggtcaaat tcaatagctg ttacctcgac gagtacatcg caaggatggt tcagatgatc
     2341 tgtctgctgt gcgtccggac cgcgtcctct gtggacatag aggtctccct gcaggtgctg
     2401 gacgccgtgg tctgctacaa ctgcctgccg gctgagagcc tcccgctgtt catcgttacc
     2461 ctctgtcgca ccatcaacgt caaggagctc tgcgagcctt gctggaagct gatgcggaac
     2521 ctccttggca cccacctggg ccacagcgcc atctacaaca tgtgccacct catggaggac
     2581 agagcctaca tggaggacgc gcccctgctg agaggagccg tgttttttgt gggcatggct
     2641 ctctggggag cccaccggct ctattctctc aggaactcgc cgacatctgt gttgccatca
     2701 ttttaccagg ccatggcatg tccgaacgag gtggtgtcct atgagatcgt cctgtccatc
     2761 accaggctca tcaagaagta taggaaggag ctccaggtgg tggcgtggga cattctgctg
     2821 aacatcatcg aacggctcct tcagcagctc cagaccttgg acagcccgga gctcaggacc
     2881 atcgtccatg acctgttgac cacggtggag gagctgtgtg accagaacga gttccacggg
     2941 tctcaggaga gatactttga actggtggag agatgtgcgg accagaggcc tgagtcctcc
     3001 ctcctgaacc tgatctccta tagagcgcag tccatccacc cggccaagga cggctggatt
     3061 cagaacctgc aggcgctgat ggagagattc ttcaggagcg agtcccgagg cgccgtgcgc
     3121 atcaaggtgc tggacgtgct gtcctttgtg ctgctcatca acaggcagtt ctatgaggag
     3181 gagctgatta actcagtggt catctcgcag ctctcccaca tccccgagga taaagaccac
     3241 caggtccgaa agctggccac ccagttgctg gtggacctgg cagagggctg ccacacacac
     3301 cacttcaaca gcctgctgga catcatcgag aaggtgatgg cccgctccct ctccccaccc
     3361 ccggagctgg aagaaaggga tgtggccgca tactcggcct ccttggagga tgtgaagaca
     3421 gccgtcctgg ggcttctggt catccttcag accaagctgt acaccctgcc tgcaagccac
     3481 gccacgcgtg tgtatgagat gctggtcagc cacattcagc tccactacaa gcacagctac
     3541 accctgccaa tcgcgagcag catccggctg caggcctttg acttcctgtt gctgctgcgg
     3601 gccgactcac tgcaccgcct gggcctgccc aacaaggatg gagtcgtgcg gttcagcccc
     3661 tactgcgtct gcgactacat ggagccagag agaggctctg agaagaagac cagcggcccc
     3721 ctttctcctc ccacagggcc tcctggcccg gcgcctgcag gccccgccgt gcggctgggg
     3781 tccgtgccct actccctgct cttccgcgtc ctgctgcagt gcttgaagca ggagtctgac
     3841 tggaaggtgc tgaagctggt tctgggcagg ctgcctgagt ccctgcgcta taaagtgctc
     3901 atctttactt ccccttgcag tgtggaccag ctgtgctctg ctctctgctc catgctttca
     3961 ggcccaaaga cactggagcg gctccgaggc gccccagaag gcttctccag aactgacttg
     4021 cacctggccg tggttccagt gctgacagca ttaatctctt accataacta cctggacaaa
     4081 accaaacagc gcgagatggt ctactgcctg gagcagggcc tcatccaccg ctgtgccagc
     4141 cagtgcgtcg tggccttgtc catctgcagc gtggagatgc ctgacatcat catcaaggcg
     4201 ctgcctgttc tggtggtgaa gctcacgcac atctcagcca cagccagcat ggccgtccca
     4261 ctgctggagt tcctgtccac tctggccagg ctgccgcacc tctacaggaa ctttgccgcg
     4321 gagcagtatg ccagtgtgtt cgccatctcc ctgccgtaca ccaacccctc caagtttaat
     4381 cagtacatcg tgtgtctggc ccatcacgtc atagccatgt ggttcatcag gtgccgcctg
     4441 cccttccgga aggattttgt ccctttcatc actaagggcc tgcggtccaa tgtcctcttg
     4501 tcttttgatg acacccccga gaaggacagc ttcagggccc ggagtactgc tctcaacgag
     4561 agacccaaga gtctgaggat agccagaccc cccaaacaag gcttgaataa ctctccaccc
     4621 gtgaaagaat tcaaggagag ctctgcagcc gaggccttcc ggtgccgcag catcagtgtg
     4681 tctgaacatg tggtccgcag caggatacag acgtccctca ccagtgccag cttggggtct
     4741 gcagatgaga actccgtggc ccaggctgac gatagcctga aaaacctcca cctggagctc
     4801 acggaaacct gtctggacat gatggctcga tacgtcttct ccaacttcac ggctgtcccg
     4861 aagaggtctc ctgtgggcga gttcctccta gcgggtggca ggaccaaaac ctggctggtt
     4921 gggaacaagc ttgtcactgt gacgacaagc gtgggaaccg ggacccggtc gttactaggc
     4981 ctggactcgg gggagctgca gtccggcccg gagtcgagct ccagccccgg ggtgcatgta
     5041 agacagacca aggaggcgcc ggccaagctg gagtcccagg ctgggcagca ggtgtcccgt
     5101 ggggcccggg atcgggtccg ttccatgtcg gggggccatg gtcttcgagt tggcgccctg
     5161 gacgtgccgg cctcccagtt cctgggcagt gccacttctc caggaccacg gactgcacca
     5221 gccgcgaaac ctgagaaggc ctcagctggc acccgggttc ctgtgcagga gaagacgaac
     5281 ctggcggcct atgtgcccct gctgacccag ggctgggcgg agatcctggt ccggaggccc
     5341 acagggaaca ccagctggct gatgagcctg gagaacccgc tcagcccttt ctcctcggac
     5401 atcaacaaca tgcccctgca ggagctgtct aacgccctca tggcggctga gcgcttcaag
     5461 gagcaccggg acacagccct gtacaagtca ctgtcggtgc cggcagccag cacggccaaa
     5521 ccccctcctc tgcctcgctc caacacagac tccgccgtgg tcatggagga gggaagtccg
     5581 ggcgaggttc ctgtgctggt ggagccccca gggttggagg acgttgaggc agcgctaggc
     5641 atggacaggc gcacggatgc ctacagcagg tcgtcctcag tctccagcca ggaggagaag
     5701 tcgctccacg cggaggagct ggttggcagg ggcatcccca tcgagcgagt cgtctcctcg
     5761 gagggtggcc ggccctctgt ggacctctcc ttccagccct cgcagcccct gagcaagtcc
     5821 agctcctctc ccgagctgca gactctgcag gacatcctcg gggaccctgg ggacaaggcc
     5881 gacgtgggcc ggctgagccc tgaggttaag gcccggtcac agtcagggac cctggacggg
     5941 gaaagtgctg cctggtcggc ctcgggcgaa gacagtcggg gccagcccga gggtcccttg
     6001 ccttccagct ccccccgctc gcccagtggc ctccggcccc gaggttacgc gatctccgac
     6061 tcggccccat cacgcagggg caagagagta gagagggacg ccttaaagag cagagccaca
     6121 gcctccaatg cagagaaagt gccaggcatc aaccccagtt tcgtgttcct gcagctctac
     6181 cattccccct tctttggcga cgagtcaaac aagccaatcc tgctgcccaa tgagtcacag
     6241 tcctttgagc ggtcggtgca gctcctcgac cagatcccat catacgacac ccacaagatc
     6301 gccgtcctgt atgttggaga aggccagagc aacagcgagc tcgccatcct gtccaatgag
     6361 catggctcct acaggtacac ggagttcctg acgggcctgg gccggctcat cgagctgaag
     6421 gactgccagc cggacaaggt gtacctggga ggcctggacg tgtgtggtga ggacggccag
     6481 ttcacctact gctggcacga tgacatcatg caagccgtct tccacatcgc caccctgatg
     6541 cccaccaagg acgtggacaa gcaccgctgc gacaagaagc gccacctggg caacgacttt
     6601 gtgtccattg tctacaatga ctccggtgag gacttcaagc ttggcaccat caagggccag
     6661 ttcaactttg tccacgtgat cgtcaccccg ctggactacg agtgcaacct ggtgtccctg
     6721 cagtgcagga aagacatgga gggccttgtg gacaccagcg tggccaagat cgtgtctgac
     6781 cgcaacctgc ccttcgtggc ccgccagatg gccctgcacg caaatatggc ctcacaggtg
     6841 catcatagcc gctccaaccc caccgatatc tacccctcca agtggattgc ccggctccgc
     6901 cacatcaagc ggctccgcca gcggatctgc gaggaagccg cctactccaa ccccagccta
     6961 cctctggtgc accctccgtc ccatagcaaa gcccctgcac agactccagc cgagcccaca
     7021 cctggctatg aggtgggcca gcggaagcgc ctcatctcct cggtggagga cttcaccgag
     7081 tttgtgtaag aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     7141 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     7201 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7261 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7321 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7381 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7441 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7501 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7561 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7621 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7681 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7741 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7801 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7861 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7921 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7981 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8041 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8101 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8161 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8221 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     8281 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     8341 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8401 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8461 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     8521 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8581 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8641 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8701 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8761 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8821 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8881 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8941 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     9001 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     9061 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     9121 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     9181 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9241 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9421 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9601 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9721 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9781 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9841 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9901 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9961 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10021 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10081 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10141 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10201 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10261 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10321 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10441 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10501 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10561 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10621 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10681 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10741 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10801 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10861 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10921 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10981 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11041 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11101 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11161 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11221 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11281 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11341 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11401 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11461 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11521 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11581 aagtgccacc tgacgtc
//
