LOCUS       sequence-379135-        2439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pInducer21 plasmid containing the human MEFV gene with the S580X 
            mutation (stop codon leading to the truncation of the B30.2 domain 
            of the pyrin protein) with a 3xFlag tag at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Chirita D, Bronnec P, Magnotti F, Dalmon S, Martin A, Popoff M, 
            Gerfaud-Valentin M, Seve P, Belot A, Contis A, Duquesne A, Nocturne 
            G, Lemelle I, Georgin-Lavialle S, Boursier G, Touitou I, Jamilloux 
            Y, Henry T
  TITLE     Mutations in the B30.2 and the central helical scaffold domains of 
            pyrin differentially affect inflammasome activation.
  JOURNAL   Cell Death Dis. 2023 Mar 25;14(3):213. doi: 
            10.1038/s41419-023-05745-9.
  PUBMED    36966139
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2023-03-25"; volume: "14(3):213. doi"; pages: " 
            10.1038/s41419-023-05745-9"
COMMENT     Sequence Label: pInducer 21-3xFlag-hMEFV-S580X
FEATURES             Location/Qualifiers
     source          1..2439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..78
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggtaccacca tggactacaa agaccatgac ggtgattata aagatcatga catcgattac
       61 aaggatgacg atgacaagct tgcggccgcg gctaagaccc ctagtgacca tctgctgtcc
      121 accctggagg agctggtgcc ctatgacttc gagaagttca agttcaagct gcagaacacc
      181 agtgtgcaga aggagcactc caggatcccc cggagccaga tccagagagc caggccggtg
      241 aagatggcca ctctgctggt cacctactat ggggaagagt acgccgtgca gctcaccctg
      301 caggtcctgc gggccatcaa ccagcgcctg ctggccgagg agctccacag ggcagccatt
      361 caggaatatt ccacacaaga aaacggcaca gatgattccg cagcgtccag ctccctgggg
      421 gagaacaagc ccaggagcct gaagactcca gaccaccccg aggggaacga ggggaacggc
      481 cctcggccgt acgggggcgg agctgccagc ctgcggtgca gccagcccga ggccgggagg
      541 gggctgtcga ggaagcccct gagcaaacgc agagagaagg cctcggaggg cctggacgcg
      601 cagggcaagc ctcggacccg gagcccggcc ctgccgggcg ggagaagccc cggcccctgc
      661 agggcgctag aggggggcca ggccgaggtc cggctgcgca gaaacgccag ctccgcgggg
      721 aggctgcagg ggctggcggg gggcgccccg gggcagaagg agtgcaggcc cttcgaagtg
      781 tacctgccct cgggaaagat gcgacctaga agccttgagg tcaccatttc tacaggggag
      841 aaggcgcccg caaatccaga aattctcctg actctagagg aaaagacagc tgcgaatctg
      901 gactcggcaa cagaaccccg ggcaaggccc actccggatg gaggggcatc tgcggacctg
      961 aaggaaggcc ctggaaatcc agaacattcg gtcaccggaa ggccaccaga cacggctgcg
     1021 agtccccgct gccacgccca ggaaggagac ccagttgacg gtacctgtgt gcgtgattcc
     1081 tgcagcttcc ccgaggcagt ttctgggcac ccccaggcct caggcagccg ctcacctggc
     1141 tgcccccggt gccaggactc ccatgaaagg aagagcccgg gaagcctaag cccccagccc
     1201 ctgccacagt gtaagcgcca cctgaagcag gtccagctgc tcttctgtga ggatcacgat
     1261 gagcccatct gcctcatctg cagtctgagt caggagcacc aaggccaccg ggtgcgcccc
     1321 attgaggagg tcgccctgga acacaagaag aaaattcaga agcagctgga gcatctgaag
     1381 aagctgagaa aatcagggga ggagcagcga tcctatgggg aggagaaggc agtgagcttt
     1441 ctgaaacaaa ctgaagcgct gaagcagcgg gtgcagagga agctggagca ggtgtactac
     1501 ttcctggagc agcaagagca tttctttgtg gcctcactgg aggacgtggg ccagatggtt
     1561 gggcagatca ggaaggcata tgacacccgc gtatcccagg acatcgccct gctcgatgcg
     1621 ctgattgggg aactggaggc caaggagtgc cagtcagaat gggaacttct gcaggacatt
     1681 ggagacatct tgcacagggc taagacagtg cctgtccctg aaaagtggac cactcctcaa
     1741 gagataaaac aaaagatcca actcctccac cagaagtcag agtttgtgga gaagagcaca
     1801 aagtacttct cagaaaccct gcgttaagaa atggaaatgt tcaatgttcc agagctgatt
     1861 ggcgctcagg cacatgctgt taatgtgatt ctggatgcag aaaccgctta ccccaacctc
     1921 atcttctctg atgatctgaa gagtgttaga cttggaaaca agtgggagag gctgcctgat
     1981 ggcccgcaaa gatttgacag ctgtatcatt gttctgggct ctccgagttt cctctctggc
     2041 cgccgttact gggaggtgga ggttggagac aagacagcat ggatcctggg agcctgcaag
     2101 acatccataa gcaggaaagg gaacatgact ctgtcgccag agaatggcta ctgggtggtg
     2161 ataatgatga aggaaaatga gtaccaggcg tccagcgttc ccccgacccg cctgctaata
     2221 aaggagcctc ccaagcgtgt gggcatcttc gtggactaca gagttggaag catctccttt
     2281 tacaatgtga cagccagatc ccacatctat acattcgcca gctgctcttt ctctgggccc
     2341 cttcaaccta tcttcagccc tgggacacgt gatggaggga agaacacagc tcctctgact
     2401 atctgtccag tgggtggtca ggggcctgac tgactcgag
//
