LOCUS       sequence-379137-       11588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, 
            K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP 
            activity; targeted to the nucleus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Zhong Y, Zhou X, Guan KL, Zhang J
  TITLE     Rheb regulates nuclear mTORC1 activity independent of farnesylation.
  JOURNAL   Cell Chem Biol. 2022 Jun 16;29(6):1037-1045.e4. doi: 
            10.1016/j.chembiol.2022.02.006. Epub 2022 Mar 15.
  PUBMED    35294906
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.02.006"; journalName: "Cell Chem Biol"; 
            date: "2022-06-16- 16"; volume: "29"; issue: "6"; pages: 
            "1037-1045.e4"
COMMENT     Sequence Label: pcDNA3-mCherry-TSC2(AA,3Q)-NLS
FEATURES             Location/Qualifiers
     source          1..11588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(979..987,988..1002)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1009..1716
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1154..1172)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1422..1441
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7093..7113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7141..7159)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7142..7159)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7179..7196)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7185..7409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7455..7883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7542..7561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7752..7773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7892..7912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7897..8226
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8077..8212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8139..8158
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8293..9087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8347..8366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8957..8976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9261..9382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9298..9317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9352..9371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9431..9447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9431..9447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9444..9466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9455..9471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9479..9485,9486..9503,9504..9509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9524..9545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9662..9679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9833..10421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9913..9932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10592..11383,11384..11452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11215..11234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11453..11557)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1081 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1141 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1201 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1261 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1321 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1381 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1441 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1501 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1561 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1621 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1681 cgccactcca ccggcggcat ggacgagctg tacaagcgca tgcatgcaag ggatccagcc
     1741 aaaccaacaa gcaaagattc aggcttgaag gagaagttta agattctgtt gggactggga
     1801 acaccgaggc caaatcccag gtctgcagag ggtaaacaga cggagtttat catcaccgcg
     1861 gaaatactga gagaactgag catggaatgt ggcctcaaca atcgcatccg gatgataggg
     1921 cagatttgtg aagtcgcaaa aaccaagaaa tttgaagagc acgcagtgga agcactctgg
     1981 aaggcggtcg cggatctgtt gcagccggag cggccgctgg aggcccggca cgcggtgctg
     2041 gctctgctga aggccatcgt gcaggggcag ggcgagcgtt tgggggtcct cagagccctc
     2101 ttctttaagg tcatcaagga ttacccttcc aacgaagacc ttcacgaaag gctggaggtt
     2161 ttcaaggccc tcacagacaa tgggagacac atcacctact tggaggaaga gctggctgac
     2221 tttgtcctgc agtggatgga tgttggcttg tcctcggaat tccttctggt gctggtgaac
     2281 ttggtcaaat tcaatagctg ttacctcgac gagtacatcg caaggatggt tcagatgatc
     2341 tgtctgctgt gcgtccggac cgcgtcctct gtggacatag aggtctccct gcaggtgctg
     2401 gacgccgtgg tctgctacaa ctgcctgccg gctgagagcc tcccgctgtt catcgttacc
     2461 ctctgtcgca ccatcaacgt caaggagctc tgcgagcctt gctggaagct gatgcggaac
     2521 ctccttggca cccacctggg ccacagcgcc atctacaaca tgtgccacct catggaggac
     2581 agagcctaca tggaggacgc gcccctgctg agaggagccg tgttttttgt gggcatggct
     2641 ctctggggag cccaccggct ctattctctc aggaactcgc cgacatctgt gttgccatca
     2701 ttttaccagg ccatggcatg tccgaacgag gtggtgtcct atgagatcgt cctgtccatc
     2761 accaggctca tcaagaagta taggaaggag ctccaggtgg tggcgtggga cattctgctg
     2821 aacatcatcg aacggctcct tcagcagctc cagaccttgg acagcccgga gctcaggacc
     2881 atcgtccatg acctgttgac cacggtggag gagctgtgtg accagaacga gttccacggg
     2941 tctcaggaga gatactttga actggtggag agatgtgcgg accagaggcc tgagtcctcc
     3001 ctcctgaacc tgatctccta tagagcgcag tccatccacc cggccaagga cggctggatt
     3061 cagaacctgc aggcgctgat ggagagattc ttcaggagcg agtcccgagg cgccgtgcgc
     3121 atcaaggtgc tggacgtgct gtcctttgtg ctgctcatca acaggcagtt ctatgaggag
     3181 gagctgatta actcagtggt catctcgcag ctctcccaca tccccgagga taaagaccac
     3241 caggtccgaa agctggccac ccagttgctg gtggacctgg cagagggctg ccacacacac
     3301 cacttcaaca gcctgctgga catcatcgag aaggtgatgg cccgctccct ctccccaccc
     3361 ccggagctgg aagaaaggga tgtggccgca tactcggcct ccttggagga tgtgaagaca
     3421 gccgtcctgg ggcttctggt catccttcag accaagctgt acaccctgcc tgcaagccac
     3481 gccacgcgtg tgtatgagat gctggtcagc cacattcagc tccactacaa gcacagctac
     3541 accctgccaa tcgcgagcag catccggctg caggcctttg acttcctgtt gctgctgcgg
     3601 gccgactcac tgcaccgcct gggcctgccc aacaaggatg gagtcgtgcg gttcagcccc
     3661 tactgcgtct gcgactacat ggagccagag agaggctctg agaagaagac cagcggcccc
     3721 ctttctcctc ccacagggcc tcctggcccg gcgcctgcag gccccgccgt gcggctgggg
     3781 tccgtgccct actccctgct cttccgcgtc ctgctgcagt gcttgaagca ggagtctgac
     3841 tggaaggtgc tgaagctggt tctgggcagg ctgcctgagt ccctgcgcta taaagtgctc
     3901 atctttactt ccccttgcag tgtggaccag ctgtgctctg ctctctgctc catgctttca
     3961 ggcccaaaga cactggagcg gctccgaggc gccccagaag gcttctccag aactgacttg
     4021 cacctggccg tggttccagt gctgacagca ttaatctctt accataacta cctggacaaa
     4081 accaaacagc gcgagatggt ctactgcctg gagcagggcc tcatccaccg ctgtgccagc
     4141 cagtgcgtcg tggccttgtc catctgcagc gtggagatgc ctgacatcat catcaaggcg
     4201 ctgcctgttc tggtggtgaa gctcacgcac atctcagcca cagccagcat ggccgtccca
     4261 ctgctggagt tcctgtccac tctggccagg ctgccgcacc tctacaggaa ctttgccgcg
     4321 gagcagtatg ccagtgtgtt cgccatctcc ctgccgtaca ccaacccctc caagtttaat
     4381 cagtacatcg tgtgtctggc ccatcacgtc atagccatgt ggttcatcag gtgccgcctg
     4441 cccttccgga aggattttgt ccctttcatc actaagggcc tgcggtccaa tgtcctcttg
     4501 tcttttgatg acacccccga gaaggacagc ttcagggccc ggagtactgc tctcaacgag
     4561 agacccaaga gtctgaggat agccagaccc cccaaacaag gcttgaataa ctctccaccc
     4621 gtgaaagaat tcaaggagag ctctgcagcc gaggccttcc ggtgccgcag catcagtgtg
     4681 tctgaacatg tggtccgcag caggatacag acgtccctca ccagtgccag cttggggtct
     4741 gcagatgaga actccgtggc ccaggctgac gatagcctga aaaacctcca cctggagctc
     4801 acggaaacct gtctggacat gatggctcga tacgtcttct ccaacttcac ggctgtcccg
     4861 aagaggtctc ctgtgggcga gttcctccta gcgggtggca ggaccaaaac ctggctggtt
     4921 gggaacaagc ttgtcactgt gacgacaagc gtgggaaccg ggacccggtc gttactaggc
     4981 ctggactcgg gggagctgca gtccggcccg gagtcgagct ccagccccgg ggtgcatgta
     5041 agacagacca aggaggcgcc ggccaagctg gagtcccagg ctgggcagca ggtgtcccgt
     5101 ggggcccggg atcgggtccg ttccatgtcg gggggccatg gtcttcgagt tggcgccctg
     5161 gacgtgccgg cctcccagtt cctgggcagt gccacttctc caggaccacg gactgcacca
     5221 gccgcgaaac ctgagaaggc ctcagctggc acccgggttc ctgtgcagga gaagacgaac
     5281 ctggcggcct atgtgcccct gctgacccag ggctgggcgg agatcctggt ccggaggccc
     5341 acagggaaca ccagctggct gatgagcctg gagaacccgc tcagcccttt ctcctcggac
     5401 atcaacaaca tgcccctgca ggagctgtct aacgccctca tggcggctga gcgcttcaag
     5461 gagcaccggg acacagccct gtacaagtca ctgtcggtgc cggcagccag cacggccaaa
     5521 ccccctcctc tgcctcgctc caacacagac tccgccgtgg tcatggagga gggaagtccg
     5581 ggcgaggttc ctgtgctggt ggagccccca gggttggagg acgttgaggc agcgctaggc
     5641 atggacaggc gcacggatgc ctacagcagg tcgtcctcag tctccagcca ggaggagaag
     5701 tcgctccacg cggaggagct ggttggcagg ggcatcccca tcgagcgagt cgtctcctcg
     5761 gagggtggcc ggccctctgt ggacctctcc ttccagccct cgcagcccct gagcaagtcc
     5821 agctcctctc ccgagctgca gactctgcag gacatcctcg gggaccctgg ggacaaggcc
     5881 gacgtgggcc ggctgagccc tgaggttaag gcccggtcac agtcagggac cctggacggg
     5941 gaaagtgctg cctggtcggc ctcgggcgaa gacagtcggg gccagcccga gggtcccttg
     6001 ccttccagct ccccccgctc gcccagtggc ctccggcccc gaggttacgc gatctccgac
     6061 tcggccccat cacgcagggg caagagagta gagagggacg ccttaaagag cagagccaca
     6121 gcctccaatg cagagaaagt gccaggcatc aaccccagtt tcgtgttcct gcagctctac
     6181 cattccccct tctttggcga cgagtcaaac aagccaatcc tgctgcccaa tgagtcacag
     6241 tcctttgagc ggtcggtgca gctcctcgac cagatcccat catacgacac ccacaagatc
     6301 gccgtcctgt atgttggaga aggccagagc aacagcgagc tcgccatcct gtccaatgag
     6361 catggctcct acaggtacac ggagttcctg acgggcctgg gccggctcat cgagctgaag
     6421 gactgccagc cggacaaggt gtacctggga ggcctggacg tgtgtggtga ggacggccag
     6481 ttcacctact gctggcacga tgacatcatg caagccgtct tccacatcgc caccctgatg
     6541 cccaccaagg acgtggacaa gcaccgctgc gaccagcagc agcacctggg caacgacttt
     6601 gtgtccattg tctacaatga ctccggtgag gacttcaagc ttggcaccat caagggccag
     6661 ttcaactttg tccacgtgat cgtcaccccg ctggactacg agtgcaacct ggtgtccctg
     6721 cagtgcagga aagacatgga gggccttgtg gacaccagcg tggccaagat cgtgtctgac
     6781 cgcaacctgc ccttcgtggc ccgccagatg gccctgcacg caaatatggc ctcacaggtg
     6841 catcatagcc gctccaaccc caccgatatc tacccctcca agtggattgc ccggctccgc
     6901 cacatcaagc ggctccgcca gcggatctgc gaggaagccg cctactccaa ccccagccta
     6961 cctctggtgc accctccgtc ccatagcaaa gcccctgcac agactccagc cgagcccaca
     7021 cctggctatg aggtgggcca gcggaagcgc ctcatctcct cggtggagga cttcaccgag
     7081 tttgtggaat tccccaaaaa gaagagaaag gtggaagacg cataatctag agggccctat
     7141 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     7201 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     7261 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     7321 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7381 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7441 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7501 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7561 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7621 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7681 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7741 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7801 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7861 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7921 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7981 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8041 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8101 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8161 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8221 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     8281 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     8341 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     8401 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8461 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8521 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8581 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8641 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8701 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8761 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8821 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8881 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8941 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     9001 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     9061 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     9121 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     9181 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     9241 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     9301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9361 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9781 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9841 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9901 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9961 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10021 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10081 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10141 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10201 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10261 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10321 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10381 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10441 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10501 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10741 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10801 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10861 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10921 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10981 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11041 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11101 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11161 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11221 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11281 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11341 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11401 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11461 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11521 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11581 ctgacgtc
//
