LOCUS       sequence-379155-        2674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#86.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM28
FEATURES             Location/Qualifiers
     source          1..2674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(561..660)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(717..733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(730..752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(905..1493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(985..1004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1629..2444)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1629..2444)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2352..2371
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2436..2455
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2565..2581
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2565..2581
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2582..2628
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gctgttagat gccgatcaaa agcaggagca
      301 taaccatgag ctgcaggaat ggctgaggca gctcaaaagt gtcttctatg atgccgaaga
      361 tgtgttgaat gaatttgagt gccaaacact gcgaaagcaa gtgctcaaag ctcatggtac
      421 catcaaagac gagatggctc aacaaatcaa agatgtcagc aagaggctag acaaggttgc
      481 agctgatagg cataagtttg gtctccgaat aattgatgtt gacacacgag tagttcatag
      541 gagagacacg agtcgcatgg acccagcttt cttgtacaaa gttggcatta taagaaagca
      601 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      661 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
      721 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      781 ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa
      841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1321 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1441 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1501 ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc
     1561 agcgtaaatg ggtagggggc ttcaaatcgt ccgctctgcc agtgttacaa ccaattaaca
     1621 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     1681 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     1741 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     1801 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     1861 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     1921 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     1981 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     2041 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     2101 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     2161 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     2221 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     2281 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     2341 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     2401 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     2461 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     2521 taacatcaga gattttgaga cacaacgtgg ctttcccccg ccgctctaga actagtggat
     2581 ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2641 gcattatacg agacgtccag gttgggatac ctga
//
