LOCUS       sequence-379156-       11118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral DSB Spectrum V3 Reporter System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   van de Kooij B, Kruswick A, van Attikum H, Yaffe MB
  TITLE     Multi-pathway DNA-repair reporters reveal competition between 
            end-joining, single-strand annealing and homologous recombination at
            Cas9-induced DNA double-strand breaks.
  JOURNAL   Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w.
  PUBMED    36075911
REFERENCE   2  (bases 1 to 11118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-8"; volume: "13(1):5295. doi"; pages: " 
            10.1038/s41467-022-32743-w"
COMMENT     Sequence Label: pLVX_DSB_Spectrum-V3
FEATURES             Location/Qualifiers
     source          1..11118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2861..2881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2903..2923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3149..3151,3152..3154,3155..3868)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3802..3823
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4529..4650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4773..5483)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5320..5338
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5480..5489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5502..5999)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5550..5569)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5941..5964
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(6087..6106)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6152..6207
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6206..6225)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             6268..6288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6310..6330
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6544..6553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6550..6552,6553..6555,6556..7014)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     misc_feature    7087..7675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7140..7160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7558..7569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7882..8515
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8643..8659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8643..8659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8656..8678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8667..8683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8691..8697,8698..8715,8716..8721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8736..8757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8874..8891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9045..9633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9125..9144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9804..10595,10596..10664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10427..10446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10665..10769)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10817..10951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 tctagagctc agatccgccg ccactatggg atcaagatcg ccaaaaaaga agagaaaggt
     2881 gccgaagaag catgcagcac caccaaaaaa aaaacgaaaa gtagaagacc cacgaggcaa
     2941 caccagcggc gtgctgagca cccccaaggc caagagggcc aagcaccccc ccggcaccga
     3001 gaagcccagg agcaggagcc agagcgagca gcccgccacc tgccccatct gctacgccgt
     3061 gatcaggcag agcaggaacc tgaggaggca cctggagctg aggcacttcg ccaagcccgg
     3121 cgtggatcca ccggtcgcct cgagcaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg
     3241 cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
     3301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctctcccacg gggtccagtg
     3361 ttttgcccgg tatcccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcacctaca agacccgcgc
     3481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3541 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3601 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3661 cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3721 cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgtgc tgagcaaaga
     3781 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3841 tctcggcatg gacgagctgt acaagtgacg cccgccccac gacccgcagc gcccgaccga
     3901 aaggagcgca cgaccccatg gctccgaccg aagccacccg gggcggcccc gccgaccccg
     3961 cacccgcccc cgaggcccac cgcgggggac acaccgaaca cgccgaccct gctgaacacg
     4021 cggcgcagtt cggtgcccag gagcggatcg aaattgatga tctattaaac aataaagatg
     4081 tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc
     4141 tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat
     4201 gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca tagttggata
     4261 tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct
     4321 atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac tttgtggttc
     4381 taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct
     4441 ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc catcagaagc
     4501 ttcagattaa cgcttacaat ttacgcctta agatacattg atgagtttgg acaaaccaca
     4561 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4621 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4681 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4741 atggctgatt atgatctaga gtcgcggccg ctttacttgt acagctcgtc catgccgccg
     4801 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
     4861 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
     4921 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
     4981 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
     5041 cagcccatgg ttttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
     5101 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
     5161 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
     5221 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
     5281 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
     5341 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
     5401 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
     5461 tcctcctcgc ccttgctcac catggtggcg gctggatcgg tcgaaaggcc cggagatgag
     5521 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
     5581 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
     5641 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
     5701 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
     5761 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
     5821 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
     5881 cgcctaccgg tggatgtgga atgtgtgagg ccagaggcca cttgtgtagc gccaagtgcc
     5941 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
     6001 gtagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     6061 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     6121 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     6181 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggg ggcccacctg
     6241 cagcccaagc tttagggatc aagatcgcca aaaaagaaga gaaaggtgcc gaagaagcat
     6301 gcagcaccac caaaaaaaaa acgaaaagta gaagacccac gaggcaacac cagcggcgtg
     6361 ctgagcaccc ccaaggccaa gagggccaag cacccccccg gcaccgagaa gcccaggagc
     6421 aggagccaga gcgagcagcc cgccacctgc cccatctgct acgccgtgat caggcagagc
     6481 aggaacctga ggaggcacct ggagctgagg cacttcgcca agcccggcgt ggatccaccg
     6541 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     6601 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6661 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6721 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     6781 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     6841 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6901 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6961 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     7021 cagaagaacg gcatcaggtg aacttcaata aaagcttggc gtaatcatgg tcaacgcgtc
     7081 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     7681 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7741 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7801 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7861 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7921 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7981 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8041 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     8101 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     8161 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8221 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     8281 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     8341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8521 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8581 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca
     8641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8701 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8821 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8881 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8941 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9001 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9061 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9121 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9181 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9241 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9301 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9361 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9421 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9481 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9541 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9601 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10261 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10681 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10741 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10801 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10921 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10981 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    11041 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    11101 tactacaaac ttagtagt
//
