LOCUS       sequence-379158-        2836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#61.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM33
FEATURES             Location/Qualifiers
     source          1..2836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(723..822)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(879..895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(879..895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(892..914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1067..1655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1147..1166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1791..2606)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1791..2606)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2514..2533
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2598..2617
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2727..2743
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2727..2743
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2744..2790
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gatcccattt gggtctcaaa acagcgaccg
      301 tgagaagaag aatgaagaca tgctaatgca agtcaccatt gattcagata tgaggttcat
      361 agatgtccac ttgggatcgc cagacaacat gaagaagtca agcatttcac atgactcttc
      421 tctcttcgag gagtgtgaga agggtactag gctgaatggt agcaagctga atttatcgga
      481 tggacggcaa gttggagaat acgtgattgg tgatgccgga tatcctcttc ttccctggct
      541 gctcacacct taccacccgg agaatgacct ctcagattat caagtagagt tcaataggag
      601 acactcagaa gcaatggccg ttgtgacgag gagtgcactg gaaagattaa aagacacatg
      661 gaagttcctt catggaggga gaacggagca cagggagagg gaagatggca ccgcccacga
      721 ggacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
      781 caacgaacag gtcactatca gtcaaaataa aatcattatt tgaggctagg tggaggctca
      841 gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt catagctgtt tcctgtgtga
      901 aattgttatc cgctcagagg gcacaatcct attccgcgct atccgacaat ctccaagaca
      961 ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag
     1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1681 ggtctgacgc tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg
     1741 gcttcaaatc gtccgctctg ccagtgttac aaccaattaa caaattctga ttagaaaaac
     1801 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     1861 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     1921 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     1981 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     2041 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     2101 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     2161 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     2221 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     2281 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     2341 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     2401 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     2461 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     2521 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     2581 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     2641 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     2701 gacacaacgt ggctttcccc cgccgctcta gaactagtgg atccaaataa aacgaaaggc
     2761 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcgcattata cgagacgtcc
     2821 aggttgggat acctga
//
