LOCUS       sequence-379159-        2677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#81.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM36
FEATURES             Location/Qualifiers
     source          1..2677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(564..663)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(720..736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(733..755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(908..1496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(988..1007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1632..2447)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1632..2447)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2355..2374
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2439..2458
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2568..2584
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2568..2584
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2585..2631
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gaaagcacgt ggttctctca aaaatgaatc
      301 agaaggaaca tcctcgggaa aaaatgaagt ttcatacgag gagctgtcta gattgatgag
      361 gataagggat ttgatgagcc tattgttgac ttatccaatg aaaatggacg agggaggtaa
      421 attaaaatcc tgcattagtg catttcctga atggaaagct cagttattgt ccactgatca
      481 tgatactatt gtgatggaca aaccatggcg tgtactaact gagaaggcac tgggatgtat
      541 gaagggattt atgcccacgg aagacccagc tttcttgtac aaagttggca ttataagaaa
      601 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
      661 ttgaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
      721 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
      781 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
      841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1501 atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa
     1561 gtcagcgtaa atgggtaggg ggcttcaaat cgtccgctct gccagtgtta caaccaatta
     1621 acaaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1681 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1741 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1801 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1861 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     1921 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     1981 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2041 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2101 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     2161 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2221 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2281 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2341 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2401 tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2461 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     2521 atgtaacatc agagattttg agacacaacg tggctttccc ccgccgctct agaactagtg
     2581 gatccaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2641 gtcgcattat acgagacgtc caggttggga tacctga
//
