LOCUS       sequence-379163-        3247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#63.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM37
FEATURES             Location/Qualifiers
     source          1..3247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1134..1233)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1290..1306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1290..1306)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1303..1325)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1478..2066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1558..1577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2202..3017)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2202..3017)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2925..2944
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3009..3028
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3138..3154
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3138..3154
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3155..3201
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gcagctggct gcatcgacaa atgatttctc
      301 gcatggcaac aaaattggaa ggggtggtta tggttctgtt tacaagggcc aattgccaag
      361 tggtctagat gttgcaataa aaattaatga caagtacaca tctcatagac ctgattttga
      421 aaatgaagtg cgaattacct caaaactcca gcatgcaaat atcattaaac ttctgggatg
      481 ctgcatggaa ggagacaaga agatattagt ctacgaatat atgccaaatg gaagtttgga
      541 ctgtctaatt gaggagggaa aaggtctcca gatcaattgg cctatatggt ttggcatagt
      601 taaaggggta gctcaaggtg tagtctatct acaccagcac tcccgactac gcgtaattca
      661 tggggatctg aaaccaagca atgttttctt ggattgtcat atgactccta ggattgctga
      721 ttttggcatt tctgaagtat taagttcagg tttagatgaa aaagagacat ctcatgtgtc
      781 aggaactctc ggttatcttg atcctgaata tattagctca ggcattatct ccaccatgag
      841 cgatttgtat gcctttggtg tcactcttct ccggataatc agtttaagac agccatatac
      901 aaattttcta gagagacgac aaagcttggt tgttcatgca tggaagttat ggtcaactgg
      961 gagaacaatg gagctgatac aaccattgtt gcatgatgaa cctgcaataa gtgatatact
     1021 caggtgcgtt cagatagcgc tgttgtgtgt tcaagagcat cgggaagaca ggcccaccat
     1081 gtcagaggtt ctccacatgc tgaactgtgg gagtgtctcc ttacctgttc ccgacccagc
     1141 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     1201 ggtcactatc agtcaaaata aaatcattat ttgaggctag gtggaggctc agtgatgata
     1261 agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat
     1321 ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac attaggtgga
     1381 gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa
     1441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2101 ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat
     2161 cgtccgctct gccagtgtta caaccaatta acaaattctg attagaaaaa ctcatcgagc
     2221 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2281 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2341 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2401 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2461 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2521 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2581 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2641 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2701 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2761 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2821 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2881 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2941 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc
     3001 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     3061 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     3121 tggctttccc ccgccgctct agaactagtg gatccaaata aaacgaaagg ctcagtcgaa
     3181 agactgggcc tttcgtttta tctgttgttt gtcgcattat acgagacgtc caggttggga
     3241 tacctga
//
