LOCUS       sequence-379168-        6471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Unique Projection Barcode (UPB7)-Pancreas for Projection-seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Zhao Q, Yu CD, Wang R, Xu QJ, Dai Pra R, Zhang L, Chang RB
  TITLE     A multidimensional coding architecture of the vagal interoceptive 
            system.
  JOURNAL   Nature. 2022 Mar;603(7903):878-884. doi: 10.1038/s41586-022-04515-5.
            Epub 2022 Mar 16.
  PUBMED    35296859
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04515-5"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7903"; pages: "878-884"
COMMENT     Sequence Label: UPB-pancreas (UPB7)
FEATURES             Location/Qualifiers
     source          1..6471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR
     promoter        442..719
                     /label=chicken beta-actin promoter
     primer_bind     998..1020
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1082..1101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1140..1149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2601..3189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2654..2674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3072..3083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3547..3668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3558..3577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3612..3631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3830..3959
                     /label=AAV2 ITR
     rep_origin      complement(4196..4784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4276..4295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4955..5746,5747..5815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5578..5597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5816..5920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5946..6401)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6083..6104)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6295..6314
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      421 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      481 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      541 tgggggcggg gggggggggg gggcgcgcgc crggsggggs ggggsggggs grggggsggg
      601 gsggggsgrg gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      661 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      721 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      781 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      841 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      901 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
      961 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1021 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
     1141 ccaccatggt gagtaagggc gaggaagtga tcaaagagtt catgcggttt aaggtgagaa
     1201 tggaaggaag catgaacggc cacgagttcg aaattgaggg agaaggagag ggacggccct
     1261 acgagggcac ccagacagcc aagctgaaag tgacaaaggg cgggcctctg ccattcgctt
     1321 gggacatcct gagcccacag tttatgtacg gctccaaggc ctatgtgaaa catccagctg
     1381 acattcccga ttataagaaa ctgagcttcc ccgaggggtt taagtgggaa agagtgatga
     1441 acttcgagga cggaggcctg gtgactgtga cccaggacag ctccctgcag gatgggaccc
     1501 tgatctacaa ggtgaaaatg agagggacaa attttccccc tgatggacct gtgatgcaga
     1561 agaaaactat gggatgggag gcctccaccg aaaggctgta tccacgcgac ggggtgctga
     1621 aaggagaaat ccaccaggct ctgaagctga aagatggggg acattacctg gtggagttca
     1681 agacaatcta catggccaag aaacctgtgc agctgccagg ctactattac gtggacacaa
     1741 aactggatat cacttcacac aacgaggact acactattgt ggagcagtat gaacggagcg
     1801 aggggagaca ccatctgttc ctgggccatg ggactggaag taccggctca gggtctagtg
     1861 gaaccgcctc aagcgaggat aacaatatgg ctgtgatcaa agagttcatg aggtttaagg
     1921 tgcgcatgga gggcagcatg aatgggcacg aatttgagat tgaaggagag ggcgaaggga
     1981 ggccttacga gggcacacag actgccaagc tgaaagtgac caagggagga ccactgcctt
     2041 tcgcttggga tatcctgtct cctcagttta tgtacggaag taaggcctat gtcaagcatc
     2101 ccgctgacat tcctgattac aagaaactgt ctttcccaga gggctttaag tgggagagag
     2161 tgatgaattt tgaagatgga ggcctggtga ccgtgacaca ggactcctct ctgcaggatg
     2221 gcactctgat ctacaaagtc aaaatgcgcg gcaccaattt tccacccgat gggcccgtga
     2281 tgcagaagaa aacaatgggg tgggaggcca gcactgaacg gctgtatcct agagacggag
     2341 tgctgaaggg cgaaatccac caggccctga agctgaaaga cggcggccac tacctggtgg
     2401 agttcaaaac catctacatg gccaagaaac cagtgcagct gcccggctat tactatgtgg
     2461 acaccaagct ggatatcaca tcccacaatg aagactacac cattgtggaa cagtatgaga
     2521 ggtctgaagg acgccaccat ctgtttctgt acggcatgga tgagctgtat aagtaagaat
     2581 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2641 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2701 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2761 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2821 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2881 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2941 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3001 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3061 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3121 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3181 cctccccgca tcgataccgt cgacaagatg gcaggcctca tgattgctgc tgcctgggta
     3241 ctgtccttcg tgctctgggc gcctgccatc ttgttctggc agtttgtggt gggtaagcgg
     3301 acggtgcccg acaaccagtg cttcatccag ttcctgtcca acccagcagt gacctttggc
     3361 acagccattg ctggcttcta cctgcctgtg gtcatcatga cggtgctgta catccacatc
     3421 tccctggcca gtcgcagccg agtccacaag caccggcccg agggcccgaa ggagaagaaa
     3481 gccaagacgc tggccttcct caagagccca ctaatgaagc agagcggccg cttcgagcag
     3541 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3601 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3661 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     3721 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta
     3781 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     3841 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3901 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     3961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5941 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6001 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6061 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6121 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6181 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6241 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6301 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6361 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     6421 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc a
//
