LOCUS       sequence-379169-       11052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral DSB Spectrum V1 Reporter System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   van de Kooij B, Kruswick A, van Attikum H, Yaffe MB
  TITLE     Multi-pathway DNA-repair reporters reveal competition between 
            end-joining, single-strand annealing and homologous recombination at
            Cas9-induced DNA double-strand breaks.
  JOURNAL   Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w.
  PUBMED    36075911
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-8"; volume: "13(1):5295. doi"; pages: " 
            10.1038/s41467-022-32743-w"
COMMENT     Sequence Label: pLVX_DSB_Spectrum-V1
FEATURES             Location/Qualifiers
     source          1..11052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2861..2881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2903..2923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     3848..3869
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4036..4157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4818..5417)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4901..4921)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5398..5417
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5436..5933)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5484..5503)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5875..5898
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(6021..6040)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6086..6141
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6140..6159)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             6202..6222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6244..6264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6478..6487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6484..6486,6487..6489,6490..6948)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     misc_feature    7021..7609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7074..7094)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7492..7503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7816..8449
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8577..8593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8577..8593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8590..8612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8601..8617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8625..8631,8632..8649,8650..8655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8670..8691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8808..8825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8979..9567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9059..9078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9738..10529,10530..10598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10361..10380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10599..10703)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10751..10885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 tctagagctc agatccgccg ccactatggg atcaagatcg ccaaaaaaga agagaaaggt
     2881 gccgaagaag catgcagcac caccaaaaaa aaaacgaaaa gtagaagacc cacgaggcaa
     2941 caccagcggc gtgctgagca cccccaaggc caagagggcc aagcaccccc ccggcaccga
     3001 gaagcccagg agcaggagcc agagcgagca gcccgccacc tgccccatct gctacgccgt
     3061 gatcaggcag agcaggaacc tgaggaggca cctggagctg aggcacttcg ccaagcccgg
     3121 cgtggatcca ccggtcgcct cgagcaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg
     3241 cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
     3301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctctcccacg gctaaggaag
     3361 atatctaggg ataacagggt aatcccgggg gcctattggt ccagtgtttt gcccggtatc
     3421 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3481 agcgcaccat cttcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg
     3541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     3661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacgtg gaggacggca
     3721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3781 tgcccgacag ccactacctg agcacccagt ccgtgctgag caaagacccc aacgagaagc
     3841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3901 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     3961 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4021 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4141 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat ctgaagcttc tgatggaatt
     4201 agaacttggc aaaacaatac tgagaatgaa gtgtatgtgg aacagaggct gctgatctcg
     4261 ttcttcaggc tatgaaactg acacatttgg aaaccacagt acttagaacc acaaagtggg
     4321 aatcaagaga aaaacaatga tcccacgaga gatctataga tctatagatc atgagtggga
     4381 ggaatgagct ggcccttaat ttggttttgc ttgtttaaat tatgatatcc aactatgaaa
     4441 cattatcata aagcaatagt aaagagcctt cagtaaagag caggcattta tctaatccca
     4501 ccccaccccc acccccgtag ctccaatcct tccattcaaa atgtaggtac tctgttctca
     4561 cccttcttaa caaagtatga caggaaaaac ttccatttta gtggacatct ttattgttta
     4621 atagatcatc aatttcgatc cgctcctggg caccgaactg cgccgcgtgt tcagcagggt
     4681 cggcgtgttc ggtgtgtccc ccgcggtggg cctcgggggc gggtgcgggg tcggcggggc
     4741 cgccccgggt ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc
     4801 gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
     4861 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
     4921 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
     4981 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
     5041 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
     5101 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
     5161 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
     5221 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
     5281 acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac
     5341 gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg
     5401 ggcttgtact cggtcatggt ggcggctgga tcggtcgaaa ggcccggaga tgaggaagag
     5461 gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac
     5521 cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt
     5581 cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg
     5641 cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct
     5701 acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact
     5761 aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta
     5821 ccggtggatg tggaatgtgt gaggccagag gccacttgtg tagcgccaag tgcccagcgg
     5881 ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa
     5941 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     6001 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     6061 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     6121 tggaattttt tgtgtctctc actcggaagg acatatggga ggggggccca cctgcagccc
     6181 aagctttagg gatcaagatc gccaaaaaag aagagaaagg tgccgaagaa gcatgcagca
     6241 ccaccaaaaa aaaaacgaaa agtagaagac ccacgaggca acaccagcgg cgtgctgagc
     6301 acccccaagg ccaagagggc caagcacccc cccggcaccg agaagcccag gagcaggagc
     6361 cagagcgagc agcccgccac ctgccccatc tgctacgccg tgatcaggca gagcaggaac
     6421 ctgaggaggc acctggagct gaggcacttc gccaagcccg gcgtggatcc accggtcgcc
     6481 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     6541 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6601 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6661 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     6721 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6781 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6841 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6901 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     6961 aacggcatca ggtgaacttc aataaaagct tggcgtaatc atggtcaacg cgtctggaac
     7021 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7081 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7141 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7201 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7261 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7321 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7381 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7441 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7501 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7561 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     7621 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7681 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7741 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7801 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7861 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     7921 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7981 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     8041 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     8101 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     8161 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8221 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8281 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8341 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8401 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8461 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8521 ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca
     8581 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8641 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8701 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8761 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8821 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8881 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8941 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9001 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9061 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9181 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9301 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9361 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9421 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9481 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9541 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9601 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9661 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9721 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9781 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9841 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9901 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9961 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10021 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10081 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10141 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10201 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10261 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10321 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10381 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10441 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10501 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10561 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10621 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10681 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10741 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10861 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    10921 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    10981 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    11041 aaacttagta gt
//
