LOCUS       sequence-379171-       11006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral DSB Spectrum V2 Reporter System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   van de Kooij B, Kruswick A, van Attikum H, Yaffe MB
  TITLE     Multi-pathway DNA-repair reporters reveal competition between 
            end-joining, single-strand annealing and homologous recombination at
            Cas9-induced DNA double-strand breaks.
  JOURNAL   Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w.
  PUBMED    36075911
REFERENCE   2  (bases 1 to 11006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-8"; volume: "13(1):5295. doi"; pages: " 
            10.1038/s41467-022-32743-w"
COMMENT     Sequence Label: pLVX_DSB_Spectrum-V2
FEATURES             Location/Qualifiers
     source          1..11006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2861..2881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2903..2923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3149..3151,3152..3154,3155..3868)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3802..3823
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3990..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4772..5371)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4855..4875)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5352..5371
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5390..5887)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5438..5457)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5829..5852
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(5975..5994)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6040..6095
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6094..6113)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             6156..6176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6198..6218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6432..6441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6438..6440,6441..6443,6444..6902)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     misc_feature    6975..7563
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7028..7048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7446..7457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7770..8403
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8531..8547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8531..8547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8544..8566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8555..8571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8579..8585,8586..8603,8604..8609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8624..8645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8762..8779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8933..9521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9013..9032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9692..10483,10484..10552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10315..10334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10553..10657)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10705..10839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 tctagagctc agatccgccg ccactatggg atcaagatcg ccaaaaaaga agagaaaggt
     2881 gccgaagaag catgcagcac caccaaaaaa aaaacgaaaa gtagaagacc cacgaggcaa
     2941 caccagcggc gtgctgagca cccccaaggc caagagggcc aagcaccccc ccggcaccga
     3001 gaagcccagg agcaggagcc agagcgagca gcccgccacc tgccccatct gctacgccgt
     3061 gatcaggcag agcaggaacc tgaggaggca cctggagctg aggcacttcg ccaagcccgg
     3121 cgtggatcca ccggtcgcct cgagcaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg
     3241 cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
     3301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctctcccacg gggtccagtg
     3361 ttttgcccgg tatcccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcacctaca agacccgcgc
     3481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3541 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3601 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3661 cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3721 cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgtgc tgagcaaaga
     3781 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3841 tctcggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata
     3901 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3961 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4021 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4081 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatctgaag
     4141 cttctgatgg aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga
     4201 ggctgctgat ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag
     4261 aaccacaaag tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata
     4321 gatcatgagt gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat
     4381 atccaactat gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca
     4441 tttatctaat cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag
     4501 gtactctgtt ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac
     4561 atctttattg tttaatagat catcaatttc gatccgctcc tgggcaccga actgcgccgc
     4621 gtgttcagca gggtcggcgt gttcggtgtg tcccccgcgg tgggcctcgg gggcgggtgc
     4681 ggggtcggcg gggccgcccc gggtggcttc ggtcggagcc atggggtcgt gcgctccttt
     4741 cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc
     4801 aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt
     4861 agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg
     4921 ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg
     4981 agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc
     5041 cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga
     5101 tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg
     5161 cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt
     5221 cttgcagctc ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg
     5281 ggtagtcggc gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg
     5341 cgaggcgcac cgtgggcttg tactcggtca tggtggcggc tggatcggtc gaaaggcccg
     5401 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
     5461 ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
     5521 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
     5581 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
     5641 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
     5701 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
     5761 ccggttggcg cctaccggtg gatgtggaat gtgtgaggcc agaggccact tgtgtagcgc
     5821 caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc
     5881 cctacccggt agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     5941 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     6001 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     6061 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggggg
     6121 cccacctgca gcccaagctt tagggatcaa gatcgccaaa aaagaagaga aaggtgccga
     6181 agaagcatgc agcaccacca aaaaaaaaac gaaaagtaga agacccacga ggcaacacca
     6241 gcggcgtgct gagcaccccc aaggccaaga gggccaagca cccccccggc accgagaagc
     6301 ccaggagcag gagccagagc gagcagcccg ccacctgccc catctgctac gccgtgatca
     6361 ggcagagcag gaacctgagg aggcacctgg agctgaggca cttcgccaag cccggcgtgg
     6421 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6481 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6541 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6601 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     6661 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6721 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6781 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6841 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6901 ccgacaagca gaagaacggc atcaggtgaa cttcaataaa agcttggcgt aatcatggtc
     6961 aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7381 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7561 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     7621 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7681 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7741 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     7801 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     7861 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     7921 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     7981 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     8041 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     8101 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     8161 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     8221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8461 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
     8521 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8581 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8641 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8701 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8761 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8821 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9121 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9181 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9241 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9301 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9361 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9421 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9481 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9541 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10681 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
    10741 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10801 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    10861 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    10921 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    10981 taaatatgta ctacaaactt agtagt
//
