LOCUS       sequence-379193-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter/biosensor for membrane tension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Hsu YY, Resto Irizarry AM, Fu J, Liu AP
  TITLE     Mechanosensitive Channel-Based Optical Membrane Tension Reporter.
  JOURNAL   ACS Sens. 2023 Jan 27;8(1):12-18. doi: 10.1021/acssensors.2c01921. 
            Epub 2023 Jan 6.
  PUBMED    36608338
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.2c01921"; journalName: "ACS Sens"; date: 
            "2023-01-27- 27"; volume: "8"; issue: "1"; pages: "12-18"
COMMENT     Sequence Label: MscL(G22S)61-cpGFP-ERexp
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     387..408
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(775..794)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgagcatta ttaaagaatt tcgcgaattt gcgatgcgcg ggaacgtggt ggatttggcg
       61 gtgagcgtca ttatcggtgc ggcattcggg aagattgtct cttcactggt tgccgatatc
      121 atcatgcctc ctctgggctt attaattggc gggatcgatt ttaaacagtt tgctgtcacg
      181 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc
      241 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
      301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc catactttcg
      361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      421 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
      481 ggcgaggagc tgttcaccgg ggtggtgccc atccaggtcg agctggacgg cgacgtaaac
      541 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
      601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      661 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      721 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
      781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      901 aacctacgcg atgcgcaggg ggatatccct gctgttgtga tgcattacgg tgtcttcatt
      961 caaaacgtct ttgattttct gattgtggcc tttgccatct ttatggcgat taagctaatc
     1021 aacaaactga atcggaaaaa agaagaacca gcagccgcac ctgcaccaac taaagaagaa
     1081 gtattactga cagaaattcg tgatttgctg aaagagcaga ataaccgctc tttttgctat
     1141 gaaaatgaag tt
//
