LOCUS       sequence-379204-        4202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Shin-ichi Arimura Lab
  TITLE     Shin-ichi Arimura Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: E1_pENTR_L1-L4_NI_G1333-DddA11-C
FEATURES             Location/Qualifiers
     source          1..4202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(414..824)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLLER
                     GTRQCIAPCMHCLHCGHAATFCNLEKLACVQL"
     primer_bind     689..705
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        715..733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(781..801)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(781..799)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(820..836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(833..855)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    844..860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(868..874,875..892,893..898))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    913..934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             1456..1707
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     protein_bind    complement(1733..1828)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1854..1872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1877..1893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1995..2014)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2006..2815
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2073..2092)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2962..3550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3451..3470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3704..3721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3880..3907)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3999..4085)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4149..4165
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4181..4202,1..78)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agcaggcttt tacccatacg atgttccaga ttacgctgat atcgccgatc
      121 tacgcacgct cggctacagc cagcagcaac aggagaagat caaaccgaag gttcgttcga
      181 cagtggcgca gcaccacgag gcactggtcg gccacgggtt tacacacgcg cacatcgttg
      241 cgttaagcca acacccggca gcgttaggga ccgtcgctgt caagtatcag gacatgatcg
      301 cagcgttgcc agaggcgaca cacgaagcga tcgttggcgt cggcaaacag tggtccggcg
      361 cacgcgctct ggaggccttg ctcacggtgg cgggagagtt gagaggtcca ccgttacagt
      421 tggacacagg ccaacttctc aagattgcaa aacgtggcgg cgtgaccgca gtggaggcag
      481 tgcatgcatg gcgcaatgca ctgacgggtg ccccgctcaa ggagacgggc gccgctacag
      541 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      601 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      661 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      721 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcctcca gcttttgttc
      781 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      841 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      901 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      961 ccaccggtcg tctccacccc tgagcaggta gtggctattg catccaacat agggggcaga
     1021 cccgcactgg agtcaattgt tgcccagtta tctcgccctg atccggcgtt ggccgcgttg
     1081 accaacgacc acctcgtcgc cttggcctgc ctcggcgggc gtcctgcgct ggatgcagtg
     1141 aaaaagggat tggggggtag tccaactcca tatcccaatt atgtgagtgc aggccatgtt
     1201 gaaggtcaat cagccctatt catgagagat aacggaataa gtgaaggact ggtgtttcac
     1261 aacaatccaa aaggtacttg tggattttgc gtaaacatga ttgaaaccct tctcccagaa
     1321 aacgctaaga tgacagtagt tccacctgaa ggtgctattc ctgtcaaaag aggtgctact
     1381 ggtgaaacca aggtgtttat tgggaattcc aattcaccca aaagcccaac gaaaggtggg
     1441 tgtagtggag gatctacaaa tctctctgac atcattgaga aagagactgg aaagcaacta
     1501 gtcattcagg agtcaatcct gatgttacca gaggaggttg aggaagtgat aggcaataag
     1561 cccgaaagcg atatacttgt tcatactgcc tatgacgaat cgacggatga gaacgtaatg
     1621 cttctaacct cagatgctcc tgagtacaaa ccttgggcgt tagttatcca ggattccaat
     1681 ggagagaaca agatcaagat gttgtgataa ctcgagcgat cctctagaca cccaactttt
     1741 ctatacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1801 actatcagtc aaaataaaat cattatttgc catccagctg cagggcggcc gcccctatag
     1861 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1921 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1981 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     2041 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     2101 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     2161 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2221 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2281 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     2341 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2401 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgccttgctc aggcgcaatc
     2461 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2521 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     2581 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     2641 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     2701 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     2761 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     2821 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     2881 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcatggtcgg
     3421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3721 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3781 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3841 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     3901 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     3961 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     4021 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     4081 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     4141 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
     4201 ac
//
