LOCUS       sequence-379206-        4202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Shin-ichi Arimura Lab
  TITLE     Shin-ichi Arimura Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: E1_pENTR_L1-L4_HD_G1333-DddA11-C
FEATURES             Location/Qualifiers
     source          1..4202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(418..828)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRASRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLLER
                     GTRQCIAPCMHCLHCGHAATFCNLEKLACVQL"
     primer_bind     693..709
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        719..737
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(785..805)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(785..803)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(824..840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(837..859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    848..864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(872..878,879..896,897..902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    917..938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             1460..1711
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     protein_bind    complement(1737..1832)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1858..1876)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1881..1897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1999..2018)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2010..2819
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2077..2096)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2966..3554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3455..3474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3708..3725
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3884..3911)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4003..4089)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4153..4169
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4185..4202,1..82)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
       61 actttgtaca aaaaagcagg cttttaccca tacgatgttc cagattacgc tgatatcgcc
      121 gatctacgca cgctcggcta cagccagcag caacaggaga agatcaaacc gaaggttcgt
      181 tcgacagtgg cgcagcacca cgaggcactg gtcggccacg ggtttacaca cgcgcacatc
      241 gttgcgttaa gccaacaccc ggcagcgtta gggaccgtcg ctgtcaagta tcaggacatg
      301 atcgcagcgt tgccagaggc gacacacgaa gcgatcgttg gcgtcggcaa acagtggtcc
      361 ggcgcacgcg ctctggaggc cttgctcacg gtggcgggag agttgagagg tccaccgtta
      421 cagttggaca caggccaact tctcaagatt gcaaaacgtg gcggcgtgac cgcagtggag
      481 gcagtgcatg catggcgcaa tgcactgacg ggtgccccgc tcaaggagac gggcgccgct
      541 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      601 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      661 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
      721 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggccc tccagctttt
      781 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
      841 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      901 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      961 ctttccaccg gtcgtctcca cccctgagca ggtagtggct attgcatccc acgacggggg
     1021 cagacccgca ctggagtcaa ttgttgccca gttatctcgc cctgatccgg cgttggccgc
     1081 gttgaccaac gaccacctcg tcgccttggc ctgcctcggc gggcgtcctg cgctggatgc
     1141 agtgaaaaag ggattggggg gtagtccaac tccatatccc aattatgtga gtgcaggcca
     1201 tgttgaaggt caatcagccc tattcatgag agataacgga ataagtgaag gactggtgtt
     1261 tcacaacaat ccaaaaggta cttgtggatt ttgcgtaaac atgattgaaa cccttctccc
     1321 agaaaacgct aagatgacag tagttccacc tgaaggtgct attcctgtca aaagaggtgc
     1381 tactggtgaa accaaggtgt ttattgggaa ttccaattca cccaaaagcc caacgaaagg
     1441 tgggtgtagt ggaggatcta caaatctctc tgacatcatt gagaaagaga ctggaaagca
     1501 actagtcatt caggagtcaa tcctgatgtt accagaggag gttgaggaag tgataggcaa
     1561 taagcccgaa agcgatatac ttgttcatac tgcctatgac gaatcgacgg atgagaacgt
     1621 aatgcttcta acctcagatg ctcctgagta caaaccttgg gcgttagtta tccaggattc
     1681 caatggagag aacaagatca agatgttgtg ataactcgag cgatcctcta gacacccaac
     1741 ttttctatac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     1801 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagggc ggccgcccct
     1861 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1921 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1981 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     2041 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2101 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     2161 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     2221 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     2281 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     2341 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2401 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctt gctcaggcgc
     2461 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2521 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2581 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2641 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2701 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2761 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2821 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     2881 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcatgg
     3421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3661 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3721 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3781 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3841 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3901 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3961 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4021 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4081 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     4141 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat
     4201 tt
//
