LOCUS       sequence-379235-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of NXPH4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Marallano et al.
  TITLE     Lentiviral vectors for functional studies in GBM cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-PGK-NXPH4 (neo)
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             283..1086
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(346..365)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     956..975
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1177..1410
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1482..1603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1519..1538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1573..1592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1643..1778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1798..1817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1799..1817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1827..1844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1827..1843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1836..1858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1985..2440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2072..2091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2282..2303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2466..2570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2571..2639,2640..3431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2789..2808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3602..4190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4091..4110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4344..4361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4478..4499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4514..4519,4520..4537,4538..4544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4552..4568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4557..4579
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4576..4592
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4576..4592
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4611..4631
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4613..4631
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4659..4885
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4886..5066
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5113..5238
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5731..5964
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6149..6193
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6460..6577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6623..7133
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7052..7070
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    7188..7212
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8134..8158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8189..8777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8242..8262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8660..8671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(8820..9050,1..269)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8855..8878)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
       61 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      121 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      181 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      241 tctcctcttc ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa
      301 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
      361 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
      421 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
      481 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
      541 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
      601 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      661 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      721 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      781 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
      841 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      901 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      961 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     1021 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     1081 ttctgagggg atccgtcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg
     1141 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac
     1201 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     1261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1381 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     1441 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     1501 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1561 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     1621 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1681 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     1741 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     1801 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     1861 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1981 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2341 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2401 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     2461 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2521 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2581 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2641 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2701 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2761 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2821 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2881 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2941 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3001 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3061 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3121 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3181 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3241 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3301 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3361 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3421 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3481 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3541 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4261 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4321 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4381 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4441 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4501 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4561 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     4621 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     4681 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     4741 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     4801 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     4861 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     4921 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4981 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5041 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     5101 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     5161 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     5221 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     5281 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     5341 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     5401 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     5461 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     5521 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     5581 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     5641 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     5701 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     5761 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     5821 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     5881 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     5941 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     6001 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     6061 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     6121 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     6181 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     6241 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     6301 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     6361 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     6421 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     6481 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     6541 agaattacaa aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga
     6601 gaagcttgat atcgaattcc acggggttgg ggttgcgcct tttccaaggc agccctgggt
     6661 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     6721 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     6781 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     6841 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     6901 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     6961 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     7021 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     7081 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     7141 caccggttcg tcgactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     7201 aaaaagcaga tgcggctgct cccggaatgg ttcctcttgc tctttggccc gtggctcctt
     7261 aggaaggccg tcagtgccca gataccagag tccggaaggc cgcagtacct ggggctgcgc
     7321 cccgccgcgg ccggagcggg tgcccccggc cagcagctcc cagagccaag gtcttcggac
     7381 ggcctaggcg tgggccgcgc ctggagctgg gcctggccga ccaaccacac gggggcgctg
     7441 gcccgggcag gggcagccgg ggcgttgccc gcgcagcgca ccaagaggaa gccgtccatc
     7501 aaggcggcgc gcgccaaaaa gatcttcggc tggggggact tctactttcg ggtgcatacc
     7561 ctcaagtttt cgctgctggt gaccggcaag atcgtggacc atgtgaacgg taccttcagt
     7621 gtgtatttcc gccacaactc gtccagcctg ggcaacctca gtgtcagcat cgtgccgccc
     7681 tccaagcgtg tcgagttcgg aggagtctgg ctgcccgggc ctgtccccca ccctctgcag
     7741 tctacgctcg ccctggaggg ggtgcttcct gggctggggc ccccgctggg gatggcagca
     7801 gcagcggcgg ggcccgggct tgggggctcc ctcgggggcg cactggcggg gccgcttggg
     7861 ggcgcgttgg gagtgcctgg ggccaaagag tcacgcgctt tcaattgcca cgtggagtat
     7921 gagaagacaa accgcgcgcg caagcaccga ccgtgcctgt acgacccgtc gcaggtgtgc
     7981 ttcaccgagc acacgcagag ccaggccgcc tggctctgtg ccaagccctt caaagtcatc
     8041 tgtatcttcg tctctttcct cagctttgac tacaaactgg tgcagaaggt gtgcccagac
     8101 tataacttcc agagtgagca cccctacttc ggatagcagc tttcttgtac aaagtggttg
     8161 atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     8221 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8281 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8341 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8401 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8461 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8521 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8581 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8641 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8701 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8761 ttgggccgcc tccccgcctg gaattctgca gatatccgga ctagtgatct aattctaccg
     8821 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     8881 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     8941 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     9001 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
//
