LOCUS       sequence-379237-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of CXCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Marallano et al.
  TITLE     Lentiviral vectors for functional studies in GBM cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-PGK-CXCR4 (neo)
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(152..220,221..1012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(370..389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1183..1771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1672..1691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1925..1942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2059..2080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2095..2100,2101..2118,2119..2125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2133..2149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2138..2160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2157..2173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2157..2173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2192..2212
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2194..2212
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2240..2466
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2467..2647
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2694..2819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3312..3545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3730..3774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4041..4158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4204..4714
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4633..4651
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4769..4793
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    5516..5527
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(5850..5874)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5905..6493
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5958..5978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6376..6387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6536..7035
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6571..6594)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6968..6987
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7049..7852
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7112..7131)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7722..7741
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7943..8176
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8248..8369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8285..8304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8339..8358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8409..8544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8564..8583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8565..8583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8593..8610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8593..8609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8602..8624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8751..9179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8838..8857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9048..9069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
       61 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      121 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      181 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      241 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      301 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      361 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      421 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      481 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      541 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      601 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      661 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      721 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      781 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      841 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      901 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
      961 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1021 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1861 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1921 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1981 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     2041 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     2101 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2161 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     2221 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     2281 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     2341 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     2401 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     2461 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     2521 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     2581 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     2641 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     2701 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     2761 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     2821 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     2881 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     2941 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     3001 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     3061 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     3121 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     3181 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     3241 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     3301 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     3361 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     3421 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     3481 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     3541 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     3601 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     3661 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     3721 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     3781 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     3841 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     3901 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     3961 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     4021 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4081 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4141 acaaaaattc aaaattttat cgatcacgag actagcctcg agaagcttga tatcgaattc
     4201 cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     4261 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     4321 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     4381 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     4441 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     4501 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     4561 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     4621 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     4681 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggttc gtcgactagt
     4741 ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagttg gcatggaggg
     4801 gatcagtata tacacttcag ataactacac cgaggaaatg ggctcagggg actatgactc
     4861 catgaaggaa ccctgtttcc gtgaagaaaa tgctaatttc aataaaatct tcctgcccac
     4921 catctactcc atcatcttct taactggcat tgtgggcaat ggattggtca tcctggtcat
     4981 gggttaccag aagaaactga gaagcatgac ggacaagtac aggctgcacc tgtcagtggc
     5041 cgacctcctc tttgtcatca cgcttccctt ctgggcagtt gatgccgtgg caaactggta
     5101 ctttgggaac ttcctatgca aggcagtcca tgtcatctac acagtcaacc tctacagcag
     5161 tgtcctcatc ctggccttca tcagtctgga ccgctacctg gccatcgtcc acgccaccaa
     5221 cagtcagagg ccaaggaagc tgttggctga aaaggtggtc tatgttggcg tctggatccc
     5281 tgccctcctg ctgactattc ccgacttcat ctttgccaac gtcagtgagg cagatgacag
     5341 atatatctgt gaccgcttct accccaatga cttgtgggtg gttgtgttcc agtttcagca
     5401 catcatggtt ggccttatcc tgcctggtat tgtcatcctg tcctgctatt gcattatcat
     5461 ctccaagctg tcacactcca agggccacca gaagcgcaag gccctcaaga ccacagtcat
     5521 cctcatcctg gctttcttcg cctgttggct gccttactac attgggatca gcatcgactc
     5581 cttcatcctc ctggaaatca tcaagcaagg gtgtgagttt gagaacactg tgcacaagtg
     5641 gatttccatc accgaggccc tagctttctt ccactgttgt ctgaacccca tcctctatgc
     5701 tttccttgga gccaaattta aaacctctgc ccagcacgca ctcacctctg tgagcagagg
     5761 gtccagcctc aagatcctct ccaaaggaaa gcgaggtgga cattcatctg tttccactga
     5821 gtctgagtct tcaagttttc actccagcta cccaactttc ttgtacaaag tggttgatat
     5881 ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     5941 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6001 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6061 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6121 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6181 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6241 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     6301 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6361 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6421 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6481 gccgcctccc cgcctggaat tctgcagata tccggactag tgatctaatt ctaccgggta
     6541 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     6601 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     6661 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     6721 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     6781 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     6841 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     6901 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     6961 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     7021 tctccgggcc tttcgacctg cagccaatat gggatcggcc attgaacaag atggattgca
     7081 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7141 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7201 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     7261 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7321 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     7381 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7441 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     7501 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     7561 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     7621 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     7681 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7741 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7801 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatcc
     7861 gtcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7921 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     7981 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8041 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8101 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8161 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8221 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     8281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8341 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8401 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8461 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8521 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8581 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8641 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8701 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8761 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8821 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8881 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8941 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9001 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9061 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9121 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9181 taaca
//
