LOCUS       sequence-379251-        9191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pseudovirus plasmid expressing BA.4 and BA.5 spike protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, 
            Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, 
            Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, 
            Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang 
            ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, 
            Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook 
            MR, Nemazee D, Mascola JR, Wilson IA, Tan J
  TITLE     Broadly neutralizing antibodies target the coronavirus fusion 
            peptide.
  JOURNAL   Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.
  PUBMED    35857439
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773."
COMMENT     Sequence Label: pcDNA3.3_SARS2_BA.4/5
FEATURES             Location/Qualifiers
     source          1..9191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(529..1320,1321..1389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1152..1171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1390..1494)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1569..1588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1760..2139
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2140..2343
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2294..2314
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2340..2364
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(6287..6306)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6331..6379
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6581..7009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6668..6687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6878..6899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7018..7038)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7023..7352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7203..7338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7265..7284
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7419..8213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7473..7492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8083..8102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8389..8510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8426..8445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8480..8499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8559..8575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8559..8575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8572..8594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8583..8599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8607..8613,8614..8631,8632..8637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8652..8673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8790..8807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8961..9191,1..358))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9041..9060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
       61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1501 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1561 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1621 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     1681 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     1741 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1801 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1861 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1921 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1981 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2041 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2101 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2161 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2221 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2281 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2341 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     2401 agaagacacc gggaccgatc cagcctccgg actgagctcc ttaaggttaa ctctagagaa
     2461 ttcggatcca tgtttgtgtt cctggtgctg ctgccactgg tgtccagcca gtgtgtgaac
     2521 ctgatcacca ggacccaatc ctacaccaac tccttcacca ggggagtcta ctaccctgac
     2581 aaggtgttca ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc
     2641 aatgtgacct ggttccatgc catctctggc accaatggca ccaagaggtt tgacaaccct
     2701 gtgctgccat tcaatgatgg agtctacttt gccagcacag agaagagcaa catcatcagg
     2761 ggctggattt ttggcaccac cctggacagc aagacccagt ccctgctgat tgtgaacaat
     2821 gccaccaatg tggtgattaa ggtgtgtgag ttccagttct gtaatgaccc attcctggac
     2881 gtctactacc acaagaacaa caagtcctgg atggagtctg agttcagggt ctactcctct
     2941 gccaacaact gtacctttga atatgtgagc caaccattcc tgatggactt ggagggcaag
     3001 cagggcaact tcaagaacct gagggagttt gtgttcaaga acattgatgg ctacttcaag
     3061 atttacagca aacacacacc aatcaacctg gggagggacc tgccacaggg cttctctgcc
     3121 ttggaaccac tggtggacct gccaattggc atcaacatca ccaggttcca gaccctgctg
     3181 gctctgcaca ggtcctacct gacacctgga gactcctcct ctggctggac agcaggagca
     3241 gcagcctact atgtgggcta cctccaacca aggaccttcc tgctgaaata caatgagaat
     3301 ggcaccatca cagatgctgt ggactgtgcc ctggacccac tgtctgagac caagtgtacc
     3361 ctgaaatcct tcacagtgga gaagggcatc taccagacca gcaacttcag ggtccaacca
     3421 acagagagca ttgtgaggtt tccaaacatc accaacctgt gtccatttga cgaggtgttc
     3481 aatgccacca ggtttgcctc tgtctatgcc tggaacagga agaggattag caactgtgtg
     3541 gctgactact ctgtgctcta caactttgcc cccttcttcg ccttcaagtg ttatggagtg
     3601 agcccaacca aactgaatga cctgtgtttc accaatgtct atgctgactc ctttgtgatt
     3661 aggggaaatg aggtgagtca gattgcccct ggacaaacag gcaacattgc tgactacaac
     3721 tacaaactgc ctgatgactt cacaggctgt gtgattgcct ggaacagcaa caagctggac
     3781 agcaaggtgg gaggcaacta caactaccgc tacagactgt tcaggaagag caacctgaaa
     3841 ccatttgaga gggacatcag cacagagatt taccaggctg gcaacaaacc atgtaatgga
     3901 gtggcgggcg tcaactgtta ctttccactc cagtcctatg gcttcagacc aacctatgga
     3961 gtgggccacc aaccatacag ggtggtggtg ctgtcctttg aactgctcca tgcccctgcc
     4021 acagtgtgtg gaccaaagaa gagcaccaac ctggtgaaga acaagtgtgt gaacttcaac
     4081 ttcaatggac tgacaggcac aggagtgctg acagagagca acaagaagtt cctgccattc
     4141 caacagtttg gcagggacat tgctgacacc acagatgctg tgagggaccc acagaccttg
     4201 gagattctgg acatcacacc atgttccttt ggaggagtgt ctgtgattac acctggcacc
     4261 aacaccagca accaggtggc tgtgctctac cagggtgtga actgtactga ggtgcctgtg
     4321 gctatccatg ctgaccaact tacaccaacc tggagggtct acagcacagg cagcaatgtg
     4381 ttccagacca gggctggctg tctgattgga gcagagtatg tgaacaactc ctatgagtgt
     4441 gacatcccaa ttggagcagg catctgtgcc tcctaccaga cccagaccaa gagccatagg
     4501 agggcaaggt ctgtggcaag ccagagcatc attgcctaca caatgagtct gggagcagag
     4561 aactctgtgg cttacagcaa caacagcatt gccatcccaa ccaacttcac catctctgtg
     4621 accacagaaa ttctgcctgt gagtatgacc aagacctctg tggactgtac aatgtatatc
     4681 tgtggagaca gcacagagtg tagcaacctg ctgctccaat atggctcctt ctgtacccaa
     4741 cttaagaggg ctctgacagg cattgctgtg gaacaggaca agaacaccca ggaggtgttt
     4801 gcccaggtga agcagattta caagacacct ccaatcaagt actttggagg cttcaacttc
     4861 agccagattc tgcctgaccc aagcaagcca agcaagaggt ccttcattga ggacctgctg
     4921 ttcaacaagg tgaccctggc tgatgctggc ttcatcaagc aatatggaga ctgtctggga
     4981 gacattgctg caagggacct gatttgtgcc cagaagttca atggactgac cgtgctgcct
     5041 ccactgctga cagatgagat gattgcccaa tacacctctg ccctgctggc tggcaccatc
     5101 acctctggct ggacctttgg agcaggagca gccctccaaa tcccatttgc tatgcagatg
     5161 gcttacaggt tcaatggcat tggagtgacc cagaatgtgc tctatgagaa ccagaaactg
     5221 attgccaacc agttcaactc tgccattggc aagattcagg actccctgtc cagcacagcc
     5281 tctgccctgg gcaaactcca agatgtggtg aaccataatg cccaggctct gaacaccctg
     5341 gtgaagcaac tttccagcaa gtttggagcc atctcctctg tgctgaatga catcctgagc
     5401 agactggaca aggtggaggc tgaggtccag attgacagac tgattacagg cagactccaa
     5461 tccctccaaa cctatgtgac ccaacaactt atcagggctg ctgagattag ggcatctgcc
     5521 aacctggctg ccaccaagat gagtgagtgt gtgctgggac aaagcaagag ggtggacttc
     5581 tgtggcaagg gctaccacct gatgagtttt ccacagtctg cccctcatgg agtggtgttc
     5641 ctgcatgtga cctatgtgcc tgcccaggag aagaacttca ccacagcccc tgccatctgc
     5701 catgatggca aggctcactt tccaagggag ggagtgtttg tgagcaatgg cacccactgg
     5761 tttgtgaccc agaggaactt ctatgaacca cagattatca ccacagacaa cacctttgtg
     5821 tctggcaact gtgatgtggt gattggcatt gtgaacaaca cagtctatga cccactccaa
     5881 cctgaactgg actccttcaa ggaggaactg gacaaatact tcaagaacca caccagccct
     5941 gatgtggacc tgggagacat ctctggcatc aatgcctctg tggtgaacat ccagaaggag
     6001 attgacagac tgaatgaggt ggctaagaac ctgaatgagt ccctgattga cctccaagaa
     6061 ctgggcaaat atgaacaata catcaagtgg ccatggtaca tctggctggg cttcattgct
     6121 ggactgattg ccattgtgat ggtgaccata atgctgtgtt gtatgacctc ctgttgttcc
     6181 tgtctgaaag gctgttgttc ctgtggctcc tgttgtaagt gactcgagcc atggaagctt
     6241 gatatctaac tgactgaacc ggtggtacca aacgggggag gctaactgaa acacggaagg
     6301 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     6361 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     6421 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     6481 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     6541 atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6781 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6901 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7261 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7321 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7381 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     7441 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7501 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7561 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     7621 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7681 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     7741 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     7801 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     7861 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7921 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     7981 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8041 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8101 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8161 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     8221 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     8281 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8341 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8401 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8521 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8581 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8641 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8701 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8761 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9181 tcggtgtagg t
//
