LOCUS       sequence-379254-         954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(Delta-TM) 
            complementation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(DadRmt2)-DadR(Delta-TM)
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      563..590
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggnancnntt cgaccgcatc ataaattctt atgatcttgt gatcatgaaa aattcgatag
       61 tgctttgaac tggggaaact ttaaccgtag gtcataaatt attctgaaac tagaatagtt
      121 tcctcaaaga gggccgctca tcttttccgt ataatcgaac ccgtcgacag aggggtcggc
      181 gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat gacggagacc gacctcatca
      241 aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc cgaagaggcc gccgactacc
      301 cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg cttcgccctg tcgccgcgcg
      361 agaccgacgt gcttccgctg ctgctggagg gccgcaccat ctcgcgcatc caggagacgc
      421 tgttcatctc ggcgggcacg gtgagcacgc atatccgcca catctaccag aagacgggcg
      481 tggacaatcg ccaggagctc atcgacctgg cccacgagac ggccgcgcag gacgaggcgg
      541 tctgaactag tcgactcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      601 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt aattcctgtt
      661 ataaaaaaag gatcaatttt gaactctctc ccaaagttga tcccttaacg atttacatta
      721 ccttctgaaa catatggcgc accttgtcca ggcggctgtt tggacggcgg ggctggatga
      781 ccggctgacc gacagcggcc tgatatcctt tcagctctgt nannnatacg ctccgcccgt
      841 tggtgtaaaa ggccagatca gtacggtatg cctgtataca gcgggcggga atctcgccag
      901 taaagacaac ttcatccttt ttacctgggc cgtttcgatg gtggcacagt antt
//
