LOCUS       sequence-379257-        9378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) 
            capsid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Wang Y, Leung VH, Zhang Y, Nudell VS, Loud M, Servin-Vences MR, Yang
            D, Wang K, Moya-Garzon MD, Li VL, Long JZ, Patapoutian A, Ye L
  TITLE     The role of somatosensory innervation of adipose tissues.
  JOURNAL   Nature. 2022 Sep;609(7927):569-574. doi: 10.1038/s41586-022-05137-7.
            Epub 2022 Aug 31.
  PUBMED    36045288
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05137-7"; journalName: "Nature"; date: 
            "2022-09"; volume: "609"; issue: "7927"; pages: "569-574"
COMMENT     Sequence Label: iCAP-ROOT
FEATURES             Location/Qualifiers
     source          1..9378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(112..131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      925..1513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1414..1433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1667..1684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1801..1822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1837..1842,1843..1860,1861..1867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1875..1891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1880..1902
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1899..1915
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1899..1915
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1934..1954
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1936..1954
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2406..2417)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    5134..5152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5171..5189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5208..5226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5245..5263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5282..5300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5319..5337
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5356..5374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5393..5411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    5473..5697
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5686..5703
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        5723..5741
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5723..5740
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(5780..5890)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     primer_bind     complement(8454..8470)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8455..8471)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8499..8518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8500..8518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8528..8545)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8528..8544)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8537..8559)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8686..9141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8773..8792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8983..9004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9167..9271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9272..9340,9341..9378,1..754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
       61 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
      841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1681 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1741 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1801 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1861 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1921 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg
     1981 tggcggccgc tctagtcgag cggccgccag tgtgatggat atctgcagaa ttcggcttgg
     2041 cggctgcgcg ttcaaacctc ccgcttcaaa atggagaccc tgcgtgctca ctcgggctta
     2101 aatacccagc gtgaccacat ggtgtcgcaa aatgtcgcaa aacactcacg tgacctctaa
     2161 tacaggacct ctagtcctgc aggtttaaac gaattcgccc ttcgcagaga ccaaagttca
     2221 actgaaacga attaaacggt ttattgatta acaagcaatt acagattacg agtcaggtat
     2281 ctggtgccaa tggggcgggg ttcactatat acaccttcag tattaacagc aaattcaaca
     2341 ttattagact tgtaatagtt ggaagtgtac tggatctccg ggttccagcg cttgctgttt
     2401 tccttctgca gctcccactc gatctccacg ctgacttggc cagtagaata ctgggtgatg
     2461 aaagagttca gcttgtcctt gttgaaggcc gttggaggat ccgcaggtac aggtgtgttt
     2521 ttgatgagga tctgaggagg cgggtgcttc attccaaacc ctcccatcag cggagaaggg
     2581 tgaaagttgc cgtccgtgtg aggaattttg gcccaaatgg gtccttgcag gtacacatct
     2641 ctgtcctgcc aaaccatacc cggaagtatt ccttggtttt gaacccaacc ggtctgcgcc
     2701 tgtgcatgat cccccatatt aggaatttgg gcactctggt ggtttgtggc cacttgtcca
     2761 taggactccg ttgctaccgg gttagtagtt ttaatttctt cttcgttggt tatcatgact
     2821 ttgtccgcat ccacgttgtc tcttccagtt ccttgtttgc caaaaattaa agatccagac
     2881 aaaggaaaga aacggtcctc tccttctttg tggctggcca tagcaggtcc aggattcatc
     2941 aagctattac gtccattgag agcccaagaa gaagctccag gccaagcaaa ttcgctgttg
     3001 ttgttttgag tcacagtggt tgagacacgt tgttgtcggt agctgggtcc aggtatgtag
     3061 tttcttccct ggacagccat gttgctgggt ccggccacac tgaattttag cgtttgttga
     3121 ttctgtccag aaccgttaat agttctagag agatagtaca agtattggtc gatgagtgga
     3181 ttcattagtc ggtccaggct ttggctgtga gcgtagctgc tatggaaagg tacgttctca
     3241 aactcgtagc tgaactggaa gttgttaccc gttcttagca tttgcgacgg gaaatattcc
     3301 aggcagtaaa aggacgaacg acccacggcc tggcttccat cattaagcgt cagatacccg
     3361 tactgaggaa tcatgaaaac gtccgctggg aacggcggga ggcagccctc gtgagccgac
     3421 ccgagcacgt acgggagctg atagtctgag tccgtgaaga cctggaccgt gctggtaagg
     3481 ttattggcga tggtcttgac tccattgttg tccgtaacct ctttgacctg aatgttgaag
     3541 agcttgaagt tgagtcgctt aggccggaat ccccagttgt tgttgatgag tcgctgccag
     3601 tcacgtggtg agaagtggca gtggaatctg ttgaagtcaa aataccccca gggggtgctg
     3661 tagccgaagt aggcgttgtc atttgaagat cctccagatg tgctgttgga gatttgcttg
     3721 tagaggtgat tgttgtaggt gggcagggcc caggttcggg tgctggtggt gatgactctg
     3781 tcccccagcc attgggaatc gcaatgccaa tttcccgagg aactacccac tccatcggca
     3841 ccttcgttat tgtctgccac tggtgcgcca ccacctgaag ccattgtaag agatcccaca
     3901 cctgaggggg ctgcgggagg ttctccgatt ggttgagggt ctgggactga ctctgtgtcg
     3961 ccagtctgac cgaaattgag tctcttttta gcgggctgtg cacccgattt gccaataccc
     4021 gcggaggagt ccggttcctg aggagactgc tctacaggcc tcttctttcc aggagccgtc
     4081 ttagccgctt cctcaaccag accaagaggt tcaagaagcc tctttttggc ctggaagact
     4141 gctcgcccga ggttgccccc aaaagacgta tcttctttga gccgctcctg gaactcggcg
     4201 tcggcgtggt tgtacttgag gtacgggttg tctccggcct tgagctgctg gtcgtaggcc
     4261 ttgtcgtgct cgagggccgc cgcgtctgct gcgttgaccg gctccccctt gtcgagtccg
     4321 ttgccgggtc caaggtattt gtaacccgga agcacaagac ctcgagcgtt gtcttgatgt
     4381 tgttgatttg ccttgggttg aggggctcca ggtttcaaag cccaccactc gcgaattcct
     4441 tcactaaggt tgtcctcgag ccaatctgga agataaccat cggcagccat acctggttta
     4501 agtcatttat tgttcagaaa cacagtcatc caagtccaca ttgaccaggt cgcaagcagt
     4561 gcaagcgtct ggcacctttc ccatgatgtg atgaatgtag cacagtttct gatacgcctt
     4621 tttgacgaca gaaacgggtt gagattctga cacgggaaag cactctaaac agtctttgac
     4681 accgtgagtg aagcagatat ttgaattctg attcagtctc tcgcattgtc tgcagggaaa
     4741 cagcatcaga ttcatgccca cgtgacgaga acatttgttt tggtacctgt ccgcgtagtt
     4801 gatcgaagct agcggtcgca gaggagcggg atcaacagtc acgtctgaac tgcgaggcgt
     4861 ctcgggaaca aatgagagcc tggcccgctt ctccagactt ttatagctag tattggtgac
     4921 gtcacccagt gggcgtttta gagatttctc cgccccttta gttcccgcca attccctggg
     4981 aactttaaac tcgtgagtca ccggcacctg attgaccttt gcccaagcaa aaaagtcctt
     5041 gacttcctgc ttagtaatct tgccaaaatc tggcgggagc cgcttagtca gttcaaattt
     5101 gaacagatct ctctgcttat atagggggta ccttctctat cactgatagg gagctcgaga
     5161 cgtgtcgact tctctatcac tgatagggag acgttctaga cgtaccttct ctatcactga
     5221 tagggagctc gagacgtgtc gacttctcta tcactgatag ggagacgttc tagacgtacc
     5281 ttctctatca ctgataggga gctcgagacg tgtcgacttc tctatcactg atagggagac
     5341 gttctagacg taccttctct atcactgata gggagctcga gacgtgtcga cttctctatc
     5401 actgataggg agacgtcagg tggcacttga tagcttgatt cgagccccag ctggttcttt
     5461 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     5521 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     5581 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     5641 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     5701 aggctgatca gcgagctcta gcatttaggt gacactatag aatagggccc tctagatcga
     5761 attcatgcat ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca
     5821 gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt
     5881 caagggcgtc ggccggcccg ccgctttcgc actttagctg tttctccagg ccacatatga
     5941 ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg
     6001 cttgtctcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta
     6061 cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag
     6121 cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca
     6181 gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat
     6241 gtgagaggag agcacagcgg aatgacttgg cgttgttccg cagaaagtct tgccatgact
     6301 cgccttccag ggggcagaag tgggtatgat gcctgtccag catctcgatg gccagggcat
     6361 cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct
     6421 tttgagcgag tttccttgtc gtcaggcctt cgataccgac ttcattgagt aattccagag
     6481 cgccgtttat gactttagat ttatccagcc gactcatacc tggtttaagt catttattgt
     6541 tcagaaacac agtcatccaa gtccacattg accaggtcgc aagcagtgca agcgtctggc
     6601 acctttccca tgatgtgatg aatgtagcac agtttctgat acgccttttt gacgacagaa
     6661 acgggttgag attctgacac gggaaagcac tctaaacagt ctttgacacc gtgagtgaag
     6721 cagatatttg aattctgatt cagtctctcg cattgtctgc agggaaacag catcagattc
     6781 atgcccacgt gacgagaaca tttgttttgg tacctgtccg cgtagttgat cgaagcttcc
     6841 gcgtctgacg tcgatggctg cgcaactgac tcgcgcaccc gtttgggctc acttatatct
     6901 gcgtcactgg gggcgggtct tttcttggct ccaccctttt tgacgtagaa ttcatgctcc
     6961 acctcaacca cgtgatcctt tgcccaccgg aaaaagtctt tgacttcctg cttggtgacc
     7021 ttcccaaagt catgatccag acggcgggtg agttcaaatt tgaacatccg gtcttgcaac
     7081 ggctgctggt gttcgaaggt cgttgagttc ccgtcaatca cggcgcacat gttggtgttg
     7141 gaggtgacga tcacgggagt cgggtctatc tgggccgagg acttgcattt ctggtccacg
     7201 cgcaccttgc ttcctccgag aatggctttg gccgactcca cgaccttggc ggtcatcttc
     7261 ccctcctccc accagatcac catcttgtcg acacagtcgt tgaagggaaa gttctcattg
     7321 gtccagttta cgcacccgta gaagggcaca gtgtgggcta tggcctccgc gatgttggtc
     7381 ttcccggtag ttgcaggccc aaacagccag atggtgttcc tcttgccgaa ctttttcgtg
     7441 gcccatccca gaaagacgga agccgcatat tggggatcgt acccgtttag ttccaaaatt
     7501 ttataaatcc gattgctgga aatgtcctcc acgggctgct ggcccaccag gtagtcgggg
     7561 gcggttttag tcaggctcat aatctttccc gcattgtcca aggcagcctt gatttgggac
     7621 cgcgagttgg aggccgcatt gaaggagatg tatgaggcct ggtcctcctg gatccactgc
     7681 ttctccgagg taatcccctt gtccacgagc cacccgacca gctccatgta cctggctgaa
     7741 gtttttgatc tgatcaccgg cgcatcagaa ttgggattct gattctcttt gttctgctcc
     7801 tgcgtctgcg acacgtgcgt cagatgctgc gccaccaacc gtttacgctc cgtgagattc
     7861 aaacaggcgc ttaaatactg ttccatatta gtccacgccc actggagctc aggctgggtt
     7921 ttggggagca agtaattggg gatgtagcac tcatccacca ccttgttccc gcctccggcg
     7981 ccatttctgg tctttgtgac cgcgaaccag tttggcaaag tcggctcgat cccgcggtaa
     8041 attctctgaa tcagtttttc gcgaatctga ctcaggaaac gtcccaaaac catggatttc
     8101 accccggtgg tttccacgag cacgtgcatg tggaagtagc tctctccctt ctcaaattgc
     8161 acaaagaaaa gagcctccgg ggccttactc acacggcgcc attccgtcag aaagtcgcgc
     8221 tgcagcttct cggccacggt caggggtgcc tgctcaatca gattcagatc catgtcagaa
     8281 tctggcggca actcccattc cttctcggcc acccagttca caaagctgtc agaaatgccg
     8341 ggcagatgct cgtcaaggtc gctggggacc ttaatcacaa tctcgtaaaa ccccggcatg
     8401 gcggctgcgc gttcaaacct cccgcttcaa aatggagacc ctgcgagctc ccccgatacc
     8461 gtcgacctcg atcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
     8521 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8581 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8641 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     8701 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8761 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8821 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8881 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8941 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9001 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9061 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     9121 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     9181 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     9241 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     9301 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     9361 agtaaaagat gctgaaga
//
