LOCUS       sequence-379281-       11991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for bidirectional optogenetic manipulation of 
            Cre-positive neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Ef1a-somBiPOLES-mCerulean-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(629..645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(642..664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    653..669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(677..683,684..701,702..707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    722..743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(860..877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1031..1619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1111..1130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1790..2581,2582..2650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2413..2432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2651..2755)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2803..2937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2840..2859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2894..2913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3105..3738
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3785..3910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4407..4640
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4825..4869
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5132..5247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5325..5536
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5537..6475
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6432..6452
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(7490..7511)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7751..7770)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8098..8119
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8519..9562
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     misc_feature    9889..10438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10033..10050)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10260..10279
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10441..11040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10441..10460)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10937..10957
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11064..11652
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11117..11137)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11535..11546)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(11859..11991,1..501)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
       61 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
      121 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
      181 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
      241 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
      301 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      361 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      421 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      481 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
      541 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga
      601 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      841 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      901 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      961 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1441 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1501 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1621 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1681 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1741 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1921 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2641 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2701 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2761 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     2821 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2881 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     2941 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     3001 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     3061 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     3121 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     3181 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     3241 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     3301 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     3361 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     3421 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     3481 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     3541 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3721 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     3781 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     3841 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     3901 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     3961 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     4021 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     4081 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     4141 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     4201 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     4261 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     4321 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     4381 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     4441 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     4501 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     4561 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     4621 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     4681 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     4741 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     4801 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     4861 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     4921 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     4981 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     5041 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     5101 cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta
     5161 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     5221 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     5281 ttatcgatga ggccctttcg tcttcactcg aggtgcccgt cagtgggcag agcgcacatc
     5341 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     5401 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5461 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5521 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     5581 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     5641 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     5701 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     5761 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     5821 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     5881 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     5941 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     6001 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     6061 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     6121 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     6181 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     6241 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     6301 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     6361 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     6421 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     6481 gtgaggatcc atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg
     6541 ctacaatatg tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt
     6601 ggtctcaggc tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc
     6661 cgtgtacctg ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac
     6721 cctgcagatg agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac
     6781 ctgtccaatc atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc
     6841 actgaatccc attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac
     6901 aatttctccc gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt
     6961 gttcgagtac agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt
     7021 cgggacacct ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt
     7081 tattgcttgg actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat
     7141 ccacgagaac gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata
     7201 tggagtgatc ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg
     7261 tctgccaggg caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa
     7321 tgcccctcct aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa
     7381 gagcaggatc accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc
     7441 ggtacccgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     7501 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     7561 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     7621 caccctcgtg accaccctga gctggggcgt gcagtgcttc gcccgctacc ccgaccacat
     7681 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     7741 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     7801 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     7861 gcacaagctg gagtacaacg ccatccacgg caacgtctat atcaccgccg acaagcagaa
     7921 gaacggcatc aaggccaact tcggcctcaa ctgcaacatc gaggacggca gcgtgcagct
     7981 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     8041 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     8101 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtatac
     8161 cggtgcagcc ctgcaggaga agaagtcatg cagccagcgc atggccgaat tccggcaata
     8221 ctgttggaac ccggacactg ggcagatgct gggccgcacc ccagcccggt gggtgtggat
     8281 cagcctgtac tatgcagctt tctacgtggt catgactggg ctctttgcct tgtgcatcta
     8341 tgtgctgatg cagaccattg atccctacac ccccgactac caggaccagt taaagtcacc
     8401 gggggtaacc ttgagaccgg atgtgtatgg ggaaagaggg ctgcagattt cctacaacat
     8461 ctctgaaaac agctctagac aggcccagat caccggacgt ccggagggag ctgtacaaga
     8521 gctgatcagc agcgccacca gatctctgtt tgccgccgga ggcatcaacc cttggcctaa
     8581 cccctaccac cacgaggaca tgggctgtgg aggaatgaca cctacaggcg agtgcttcag
     8641 caccgagtgg tggtgtgacc cttcttacgg actgagcgac gccggatacg gatattgctt
     8701 cgtggaggcc acaggcggct acctggtcgt gggagtggag aagaagcagg cttggctgca
     8761 cagcagaggc acaccaggag aaaagatcgg cgcccaggtc tgccagtgga ttgctttcag
     8821 catcgccatc gccctgctga cattctacgg cttcagcgct tggaaggcca cttgcggttg
     8881 ggaggaggtc tacgtctgtt gcgtcgaggt gctgttcgtg accctggaga tcttcaagga
     8941 gttcagcagc cccgccacag tgtacctgtc taccggcaac cacgcctatt gcctgcgcta
     9001 cttcgagtgg ctgctgtctt gccccgtgat cctgatcaag ctgagcaacc tgagcggcct
     9061 gaagaacgac tacagcaagc ggaccatggg cctgatcgtg tcttgcgtgg gaatgatcgt
     9121 gttcggcatg gccgcaggac tggctaccga ttggctcaag tggctgctgt atatcgtgtc
     9181 ttgcatctac ggcggctaca tgtacttcca ggccgccaag tgctacgtgg aagccaacca
     9241 cagcgtgcct aaaggccatt gccgcatggt cgtgaagctg atggcctacg cttacttcgc
     9301 ctcttggggc agctacccaa tcctctgggc agtgggacca gaaggactgc tgaagctgag
     9361 cccttacgcc aacagcatcg gccacagcat ctgcgacatc atcgccaagg agttttggac
     9421 cttcctggcc caccacctga ggatcaagat ccacgagcac atcctgatcc acggcgacat
     9481 ccggaagacc accaagatgg agatcggagg cgaggaggtg gaagtggaag agttcgtgga
     9541 ggaggaggac gaggacacag tgctcgagca gagccagccc atcctgaaca ccaaggagat
     9601 ggccccccag agcaagcccc ccgaggagct ggagatgagc agcatgccca gccccgtggc
     9661 ccccctgccc gcccgcaccg agggcgtgat cgacatgcgc agcatgagca gcatcgacag
     9721 cttcatcagc tgcgccaccg acttccccga ggccacccgc ttctgatcta gaaagctatt
     9781 cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag
     9841 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
     9901 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     9961 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
    10021 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
    10081 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
    10141 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
    10201 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
    10261 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
    10321 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
    10381 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
    10441 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
    10501 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
    10561 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
    10621 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
    10681 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
    10741 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
    10801 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
    10861 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
    10921 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
    10981 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
    11041 cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
    11101 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
    11161 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
    11221 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
    11281 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    11341 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    11401 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
    11461 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
    11521 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    11581 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
    11641 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
    11701 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
    11761 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
    11821 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
    11881 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
    11941 gcagaactac acaccagggc caggggtcag atatccactg acctttggat g
//
