LOCUS       sequence-379284-       10731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mApple
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(554..1345,1346..1414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1177..1196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1415..1519)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1546..2888
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2509..2556)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2929..2947
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2985..3007)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3107..3126
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3150..3365
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3366..4169
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(4300..4755)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4437..4458)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4649..4668
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             7682..7693
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8182..8892
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     8595..8614
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(10137..10159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10148..10164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10172..10178,10179..10196,10197..10202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10217..10238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10355..10372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10526..10731,1..383))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10606..10625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
       61 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg
     1561 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     1621 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     1681 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     1741 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     1801 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     1861 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     1921 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     1981 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     2041 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     2101 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     2161 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     2221 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     2281 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     2341 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     2401 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
     2461 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     2521 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
     2581 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
     2641 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
     2701 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
     2761 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
     2821 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
     2881 attggatcat actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2941 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     3001 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3061 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3121 attgtactga gagtgcacca taccaccttt tcaattcatc attttttttt tattcttttt
     3181 tttgatttcg gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac
     3241 gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat
     3301 gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat
     3361 aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg
     3421 ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc
     3481 gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa
     3541 aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg
     3601 cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta
     3661 atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta
     3721 cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag
     3781 aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc
     3841 tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg
     3901 ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga
     3961 ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa
     4021 ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg
     4081 caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat
     4141 atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac
     4201 taaactcaca aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa
     4261 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
     4321 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     4381 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     4441 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     4501 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4561 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     4621 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4681 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4741 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4801 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     4861 ttgggtaacg ccagggtttt cccagtcacg acgctggaga agctcattcc acccgaaatg
     4921 cccgtgaact tgcccttgtt tgcgcgggcc ttcttgcgct ctgctcggat acgctcgaca
     4981 tcgctgagga gctcggcgag ttccttggcg cggttgcgga cgttgatacc ctggtctttg
     5041 ccgttctggt cgataaaatg gaactggcgc agcatcttga ggagggtaag atgcgaccga
     5101 gcatcgtcca ccactcgttc ggaaccgtgc ttaatcaggt actctagtag ttgaagggcc
     5161 ttgtagatct ggcgccattc ttcggcggac ttttctgtga aacggcggta aatcatgggc
     5221 atgatttcgt tgagtgtttg gctaagaaca aggagggaaa cgcaagttag ccgcgttcgt
     5281 atacaagcga gcagttctcg agaacgtact agttgaaggt gccatcggcg atctcttgca
     5341 tcaaagtcga cgatgcaccc caaggttcat tgttggtggc ctcgcgcacc tgggttcgct
     5401 gtcagctccc gttccatggt ctcttgtctg ggatcaagta tatggcgaag ggcgcaagag
     5461 aatggtgcca gtgtggcgcg tggatacggg cataccttgg actccatctc ggtatagttc
     5521 atgacagctg gttggagcca ttgtcagcac cctgaacacg aaatcatgag ggagtcttgg
     5581 ttctggacta ggtggtcacc taccattttg aacctttcgt acgccagcct tgatgtcgta
     5641 gagtgtcagg ttggacacgg tatccttgag gctattgaaa tccattgcgt aaacgtgtct
     5701 atcgccggag ttgctgggaa ttgttctgat gggctaaggt aattcttctc tagatccaat
     5761 ctgagcctcg cggtgatgat tgccagacgg gcgggatgct gtggcgactt ggtggttttt
     5821 gttcgggctg atcgctttca gggcgacgtt tcacggtctt gtcgggatca gatggacatt
     5881 gaggtgcgga attatacgat ttaggtgact gcaggccata cagatctccc tagacgccgg
     5941 gccaatccgt tccttgcacc ttctctcaca catgcaggtt ttggaatgtg gcttcatcgt
     6001 gtccagaact gggcgcattg gggccaggct cagtgtcagt gtagggatcg atgtacatat
     6061 ggcagttgac agcgagagag ccaggtgtca caggcagtcc gtcagtggcc gcggtggtgg
     6121 ccacgcactt tctgcaggag ccgtctgggg tgcgtgcagc tggcggccgc ttccgggcat
     6181 acggcttgtg taacgagggt gttgaccctc ggcttggcgc ttgaacttgg cgctgcggtc
     6241 cccccacagt tgaccacaat ccccggccaa tcgaacgacg gcaacaaggg cgcactagcc
     6301 agtgaacgcc tggggatagt gggtggtcaa agagaattgg gcaaacgaga cgacaatgga
     6361 gggtgaaccg ggaataataa acagctggtg agaccaccca tcaaacgaat caccccacca
     6421 tgggatgccc tcagatgccc tccatgccac cataaggaat gatcttacgg cttgccgaag
     6481 ttaacaggcc tgcttttgca tatgaacgag agcctggtgt tgcattccac attggctatc
     6541 tacagtacct acccaaccta cacaccctga tgcgaaaggg acagaagggg agaaggatac
     6601 atacatatac ctgactcgtg cccatcaaca acagagaaag ggtgatcgct gaacacatca
     6661 gagcatctct tcccccctgg cgactaaaag tgctcccaat ctcgtcttac atctgatacg
     6721 ttgttcaaac tccgcaaccc gatctgcaca ctgccctgcc ttggcccttc ttggagtctc
     6781 cgcttcccca cgccatctca cgctcgagga ctccgctgct aggtatgagc taggcctgcc
     6841 gtcgacagga tccaccagac gtcaacaata acccatcttg gaaatacata agaagacgtc
     6901 acttccctcc cttttcgaaa ggaagagatc atcatcagca tccatcatgg ctggtctttt
     6961 tggttccgcc gccgcatccg cgagcaacac cctcggcgat cttaagcagg atgtcgagct
     7021 cggccaaccc cccgaggaca gcatcagcga ccttgccttc aaccccaacc ccgccgacca
     7081 gaaggacttc ctcgccgtag ccagctggga caagaagaca cgcatttacg agatcttgag
     7141 taatggtcaa ggtcagggac aggccatgat tgagcatgat gcccccgttt tctcttgcga
     7201 tttcttcaag gtatgtttgc gtcgattggc ctggcaggta gcccgacaaa acactgacat
     7261 gcacttctcc caacaggacg gcacaaaggt catttccgcc ggcgccgaca aggccgccaa
     7321 ggttctcgat ttggccaccg gtcagtcgat gcaggtcgct gctcacgata tgcccatcaa
     7381 gtgcgttcgc tacttcgagg ccaatggcac gcccatggcg gtgacaggtg gctgggacaa
     7441 gcagatcaag tactgggatt tccgctccgc gaacccggcc gccacggtgc aggcccagga
     7501 gcgcgtttat accatggatg tgcgcgacaa cttgctcgtg gtcggaaccg ctgatcgcta
     7561 tatcaacgtg atcaacctga aggaccccgg caagttctac aagacaatgc agagccctct
     7621 taaatggcag actcgtgtgg tcagctgctt caacgattct cagggctttg ctatcggctc
     7681 gatcgagggt agatgcgcca ttcaatatgt ggaggacaag gattctgcgt acgccttcac
     7741 ctcctccccc tttactatcc catctgccga atacgctgac taactgactt gttttccagc
     7801 tccaacttct ctttcaaatg ccaccgcgac cccgcccaag gcaacaccac ggccgtccac
     7861 gctgtcaacg acatctcctt ccacccccaa cacggcacct tcagcacagc cggtagcgac
     7921 ggcaccttcc acttctggga caaggacgcc aagcaccgtc tcaagggtta ccccaacgtc
     7981 ggcggcagca tcacctcgac aacgttcaac aagaccggca gcatctttgc ctacgccatt
     8041 agttatgact ggagcaaggg gtaccaaggc aactcgccca cgtacccgac caaggtcatg
     8101 ctccacccag tacagcagga cgagtgcaag ccccgtccga gcgttaagaa gagggctggt
     8161 attggtttgg ctattgctgg catggtgagc aagggcgagg agaataacat ggccatcatc
     8221 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     8281 atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg
     8341 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     8401 tccaaggtct acattaagca cccagccgac atccccgact acttcaagct gtccttcccc
     8461 gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac
     8521 caggactcct ccctgcagga cggcgtgttc atctacaagg tgaagctgcg cggcaccaac
     8581 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccgaggag
     8641 cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaagaggct gaagctgaag
     8701 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     8761 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
     8821 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     8881 ctgtacaagt gactagagga tccgacacta tagaatacct agacagtttg caggtcatgt
     8941 gaatgttatc gccaacacat tgaatgctaa acagattacc tccaacgcct agatgaaacc
     9001 aaattaccct tgccttcttt ttttttacgc cgttaatgca gttgtgatag agaccatgca
     9061 acgtcttcta aacggagaca caaacacatt cgagtcgatc agatcgaaat gacttgttcc
     9121 tgtacctcgt caccatccag ccaccgtctg aaatagacgc agttgctggg gctgacaggt
     9181 ccgccttcct cgcacatatc atagacaggg caccgcccac aaggcgcctc cataaggcca
     9241 cttgcactcg gctcaactcc cataaatgag gtaacaatgc ctcctttggt tggatcatgg
     9301 tcgaggcgtc gttgattgca cgtcctcgga tcctgtttgg ggatgcgcgt cacacggtac
     9361 cccttgcgcc cattgacctt gacaggctct actaagccat cgtagtatag cacatccacc
     9421 aactgctgga tatccgcctc gctcaacgtc gtattattcg tgatgccggc gtggtggatg
     9481 aactttgcaa tgtccgacac agtcgggtac gacttgtaac cggccgggag aggcagcaat
     9541 tgcggcttgc cgggcctggt cttcttggcc ggtgccggtg ccgccgtgtc gtcgttggaa
     9601 atctcgcccg cgctgcgctt cgtccccttg gaaatatcct ccgagtgggg aatggtcccc
     9661 tgaaccacgc ccttcttggg attcttttca cgctgctgtg ctcctcctcc ttgtgctcct
     9721 actcctggta ctccagcacc gccgccgtgg gaagtatatg tcgatttctg cttgataaag
     9781 tcaaacacaa tttcctgtag ctcgttgatg aaggccgtgt cgagctcgcc ctcagtaaac
     9841 caggggccac ctgtggcgcg atcggaggga cgtaggttcg ccttgatgta catcttcttg
     9901 ttggggtgct cgacgttctt catgctggcg atgtagccct tttgctcgag atacttgatg
     9961 caatgcttca taagcgagtc ggccatgttg agcttgttct tgatggtccg gttccagatg
    10021 ccgtcggccc cggcttcgtc gaccatggcg tataccatca ttatttcgga gttggggagg
    10081 gaggtgtacc ttttgggagg aaggggaggt tagttggtgt gctcggttgg ctgtttcctg
    10141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10321 gaggcggttt gtgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc t
//
