LOCUS       sequence-379292-        6463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-vector for optogenetic silencing of noradrenergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Maheu M, Donner TH, Wiegert JS
  TITLE     Serotonergic and noradrenergic interactions in pupil-linked arousal
  JOURNAL   bioRxiv 2025.03.26.644382
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.26.644382"
COMMENT     Sequence Label: PRS_8-Jaws-tdTomato
FEATURES             Location/Qualifiers
     source          1..6463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2801..3389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2854..2874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3272..3283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3558..3639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3595..3614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3649..3668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3718..3858
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3718..3847
                     /label=AAV2 ITR
     rep_origin      3933..4388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4020..4039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4230..4251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4405..4424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4524..4546
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4584..4602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4670..4774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4775..4843,4844..5635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4993..5012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5806..6394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6295..6314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(6456..6463,1..122)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc
       61 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
      121 ctgcggccgc acgcgtttaa ttaaaaacgc gtattagctt ccgctagaca aatgtgatta
      181 cccccgctag acaaatgtga ttacccgcgc tagacaaatg tgattacccc gctagacaaa
      241 tgtgattacc ccccgctaga caaatgtgat tacccccgct agacaaatgt gattacccgc
      301 gctagacaaa tgtgattacc ccgctagaca aatgtgatta cccccgacca gggcataaat
      361 ggccaggtgg gaccagagat atcgctagca aggatccgcc accatgaccg ccgtgagcac
      421 cacagccact accgtgctgc aggccacaca gagcgacgtg ctgcaggaga tccagtccaa
      481 cttcctgctg aatagctcca tctgggtgaa cattgctctg gccggagtgg tcatcctgct
      541 gtttgtggcc atggggaggg atctggaatc ccctagagct aagctgatct gggtggccac
      601 aatgctggtg ccactggtgt ctatttctag ttacgctgga ctggccagtg ggctgactgt
      661 gggcttcctg cagatgccac ctggacacgc tctggccgga caggaggtgc tgagcccatg
      721 gggccggtat ctgacatgga ctttctccac tcccatgatc ctgctggctc tgggactgct
      781 ggccgacacc gatattgcca gcctgttcac cgccatcaca atggacattg gcatgtgcgt
      841 gacaggactg gccgctgccc tgatcactag ctcccatctg ctgcgctggg tgttctacgg
      901 aatttcttgt gctttctttg tggccgtgct gtatgtgctg ctggtgcagt ggccagctga
      961 tgctgaggct gctgggacca gtgaaatctt tggcactctg aggattctga ccgtggtgct
     1021 gtggctgggg taccctatcc tgttcgctct gggctctgag ggagtggccc tgctgagtgt
     1081 gggagtgacc agctggggat actccggact ggacatcctg gctaaatacg tgttcgcctt
     1141 tctgctgctg agatgggtgg ctgccaatga aggcacagtg tctgggagtg gaatgggaat
     1201 cgggtccgga ggagctgctc cagccgacga tcgaccggta gtaaaatcca gaattacttc
     1261 tgaaggggag tatatccctc tggatcaaat agacatcaat gttgcacctg caggggcagt
     1321 gagtaagggc gaggaagtga tcaaagagtt catgcggttt aaggtgagaa tggaaggaag
     1381 catgaacggc cacgagttcg aaattgaggg agaaggagag ggacggccct acgagggcac
     1441 ccagacagcc aagctgaaag tgacaaaggg cgggcctctg ccattcgctt gggacatcct
     1501 gagcccacag tttatgtacg gctccaaggc ctatgtgaaa catccagctg acattcccga
     1561 ttataagaaa ctgagcttcc ccgaggggtt taagtgggaa agagtgatga acttcgagga
     1621 cggaggcctg gtgactgtga cccaggacag ctccctgcag gatgggaccc tgatctacaa
     1681 ggtgaaaatg agagggacaa attttccccc tgatggacct gtgatgcaga agaaaactat
     1741 gggatgggag gcctccaccg aaaggctgta tccacgcgac ggggtgctga aaggagaaat
     1801 ccaccaggct ctgaagctga aagatggggg acattacctg gtggagttca agacaatcta
     1861 catggccaag aaacctgtgc agctgccagg ctactattac gtggacacaa aactggatat
     1921 cacttcacac aacgaggact acactattgt ggagcagtat gaacggagcg aggggagaca
     1981 ccatctgttc ctgggccatg ggactggaag taccggctca gggtctagtg gaaccgcctc
     2041 aagcgaggat aacaatatgg ctgtgatcaa agagttcatg aggtttaagg tgcgcatgga
     2101 gggcagcatg aatgggcacg aatttgagat tgaaggagag ggcgaaggga ggccttacga
     2161 gggcacacag actgccaagc tgaaagtgac caagggagga ccactgcctt tcgcttggga
     2221 tatcctgtct cctcagttta tgtacggaag taaggcctat gtcaagcatc ccgctgacat
     2281 tcctgattac aagaaactgt ctttcccaga gggctttaag tgggagagag tgatgaattt
     2341 tgaagatgga ggcctggtga ccgtgacaca ggactcctct ctgcaggatg gcactctgat
     2401 ctacaaagtc aaaatgcgcg gcaccaattt tccacccgat gggcccgtga tgcagaagaa
     2461 aacaatgggg tgggaggcca gcactgaacg gctgtatcct agagacggag tgctgaaggg
     2521 cgaaatccac caggccctga agctgaaaga cggcggccac tacctggtgg agttcaaaac
     2581 catctacatg gccaagaaac cagtgcagct gcccggctat tactatgtgg acaccaagct
     2641 ggatatcaca tcccacaatg aagactacac cattgtggaa cagtatgaga ggtctgaagg
     2701 acgccaccat ctgtttctgt acggcatgga tgagctgtat aaggtagcat tctgctacga
     2761 gaatgaagtg taataggaat tccgtacaaa gcttatcgat aatcaacctc tggattacaa
     2821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3181 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat
     3241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3361 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctgt
     3421 gatagcggcc atcaagctgg ccgcgactct agatcataat cagccatacc acatttgtag
     3481 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3541 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3601 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3661 aactcatcaa tgtatcagct tatcgatacc gcatgcacgt gcggaccgag cggccgcagg
     3721 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3781 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3841 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3901 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4141 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4261 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4381 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4501 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4561 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6421 gttcctggcc ttttgctggc cttttgctca catgtcctgc agg
//
