LOCUS       sequence-379294-        6969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of excitatory 
            neurons with blue shifted excitation spectrum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CaMKII-somBiPOLES-ChroME2f-mCerulean
FEATURES             Location/Qualifiers
     source          1..6969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   8..137
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(1534..1555)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1795..1814)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2142..2163
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3751..4154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    4161..4209
                     /note="synthetic polyadenylation signal"
     repeat_region   4239..4379
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4239..4368
                     /label=AAV2 ITR
     rep_origin      4454..4909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4541..4560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4751..4772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4926..4945)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5045..5067
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5105..5123)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5191..5295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5296..5364,5365..6156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5514..5533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6327..6915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6816..6835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
       61 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      121 catcactagg ggttcctgcg gccgcacgcg tcttgtggac taagtttgtt cgcatcccct
      181 tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc ctgggcccac
      241 acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt ttaaagtgaa
      301 aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg tttcggaggt
      361 ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca gccacagtgc
      421 cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac tcaggagcac
      481 gggcaggcga gtggccccta gttctggggg cagctctaga gcggtaccgg atccatggca
      541 tcacaggtcg tctatgggga atgggcatca actcacaccg agtgctacaa tatgtccaga
      601 atcgactcca ccttcgtctc actgctgcag ctggtgtggg ccgtggtctc aggctgccag
      661 acaatcttca tgattagcag ggctcccaag gtcccttggg agtccgtgta cctgcccttt
      721 gtcgaatcta tcacttatgc actggccagt actggcaacg gaaccctgca gatgagggac
      781 ggcagattct ttccttggag ccgaatggcc tcctggctgt gcacctgtcc aatcatgctg
      841 ggacagattt ctaacatggc tctggtgaag tacaaaagta tcccactgaa tcccattgca
      901 caggccgctt ccatcattag agtggtcatg ggaatcaccg ctacaatttc tcccgcagag
      961 tatatgaagt ggctgttctt tttctttggg gccacctgcc tggtgttcga gtacagtgtg
     1021 gtcttcacaa tctttcaagt ggggctgtat ggctttgaat ctgtcgggac acctctggca
     1081 cagaaggtgg tggtgaggat caagatgctg cgcctgatct tctttattgc ttggactatg
     1141 ttcccaatcg tgtggctgat ttcacccacc ggagtctgcg tgatccacga gaacgtgagc
     1201 gccattctgt acctgctggc tgacggcctg tgcaaaaata catatggagt gatcctgtgg
     1261 agcactgcct ggggagtgct ggaagggaag tgggaccccg catgtctgcc agggcaggag
     1321 aaacccgaag ccgacgatcc ttttggcctg aatcacgaga agaatgcccc tcctaacgac
     1381 gaagtcaata tcagaatgtt tggaagggga cgtacggcgg ccaagagcag gatcaccagc
     1441 gagggcgagt acatccccct ggaccagatc gacatcaacg tggcggtacc cgtgagcaag
     1501 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1561 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1621 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1681 ctgagctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1741 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1801 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1861 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1921 aacgccatcc acggcaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1981 aacttcggcc tcaactgcaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2041 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2101 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2161 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt ataccggtgc agccctgcag
     2221 gagaagaagt catgcagcca gcgcatggcc gaattccggc aatactgttg gaacccggac
     2281 actgggcaga tgctgggccg caccccagcc cggtgggtgt ggatcagcct gtactatgca
     2341 gctttctacg tggtcatgac tgggctcttt gccttgtgca tctatgtgct gatgcagacc
     2401 attgatccct acacccccga ctaccaggac cagttaaagt caccgggggt aaccttgaga
     2461 ccggatgtgt atggggaaag agggctgcag atttcctaca acatctctga aaacagctct
     2521 agacaggccc agatcaccgg acgtccggag ggagctgtac aagaaacagc cgccacaatg
     2581 acccacgcct ttatctcagc cgtgcctagc gccgaagcca caattagagg cctgctgagc
     2641 gccgcagcag tggtgacacc agcagcagac gctcacggag aaacctctaa cgccacaaca
     2701 gccggagccg atcacggttg cttcccccac atcaaccacg gaaccgagct gcagcacaag
     2761 atcgcagtgg gactccagtg gttcaccgtg atcgtggcta tcgtgcagct catcttctac
     2821 ggttggcaca gcttcaaggc cacaaccggc tgggaggagg tctacgtctg cgtgatcgag
     2881 ctcgtcaagt gcttcatcga gctgttccac gatgtcgaca gcccagccac agtgtaccag
     2941 accaacggag gagccgtggc ttggctgcgg tacagcgagt ggctcctgac ttgccccgtg
     3001 atcctgatcc acctgagcaa cctgaccgga ctgcacgaag agtacagcaa gcggaccatg
     3061 accatcctgg tgaccgacat cggcaacatc gtgtggggga tcacagccgc ctttacaaag
     3121 ggccccctga agatcctgtt cttcatgatc ggcctgttct acggcgtgac ttgcttcttc
     3181 cagatcgcca aggtgtatat cgagagctac cacaccctgc ccaaaggcgt ctgccggaag
     3241 atttgcaaga tcatggccta cgtcttcttc tgctcttggc tgatgttccc cgtgatgttc
     3301 atcgccggac acgagggact gggcctgatc acaccttaca ccagcggaat cggccacctg
     3361 atcctggatc tgatcgccaa gaacacttgg ggcttcctgg gccaccacct gagagtgaag
     3421 atccacgagc acatcctgat ccacggcgac atccggaaga caaccaccat caacgtggcc
     3481 ggcgagaaca tggagatcga gaccttcgtc gacgaggagg aggagggagg agtgctcgag
     3541 cagagccagc ccatcctgaa caccaaggag atggcccccc agagcaagcc ccccgaggag
     3601 ctggagatga gcagcatgcc cagccccgtg gcccccctgc ccgcccgcac cgagggcgtg
     3661 atcgacatgc gcagcatgag cagcatcgac agcttcatca gctgcgccac cgacttcccc
     3721 gaggccaccc gcttctgatc tagaaagctt aatcaacctc tggattacaa aatttgtgaa
     3781 agattgactg atattcttaa ctatgttgct ccttttacgc tgtgtggata tgctgcttta
     3841 atgcctctgt atcatgctat tgcttcccgt atggctttcg ttttctcctc cttgtataaa
     3901 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tccgtcaacg tggcgtggtg
     3961 tgctctgtgt ttgctgacgc aacccccact ggctggggca ttgccaccac ctgtcaactc
     4021 ctttctggga ctttcgcttt ccccctcccg atcgccacgg cagaactcat cgccgcctgc
     4081 cttgcccgct gctggacagg ggctaggttg ctgggcactg ataattccgt ggtgttgtcg
     4141 gggaagctga cgtcagcgct aataaaatcg ctatccatcg aagatggatg tgtgttggtt
     4201 ttttgtgtgg gtaacccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     4261 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4321 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4381 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4441 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4501 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     4561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     4801 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     4861 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4921 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4981 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5041 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5101 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5161 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5221 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5281 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5341 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5401 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5461 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5521 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5581 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5641 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5701 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5761 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5821 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5881 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5941 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6001 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6061 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6121 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6181 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6241 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6301 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6361 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6421 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6481 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6541 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6601 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6661 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6721 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6781 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6841 cctgtcgggt tttgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6901 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6961 ccttttgct
//
