LOCUS       sequence-379295-        7398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-vector for optogenetic bidirectional manipulation of 
            noradrenergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  TITLE     PRSx8-Plasmids
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRS_8-somBiPOLES-mCerulean
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2090)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2281..2300
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2459..2588
                     /label=AAV2 ITR
     primer_bind     complement(3913..3934)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4174..4193)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4521..4542
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4942..5985
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     misc_feature    6208..6796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6261..6281)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6679..6690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6837..7044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7214..7343
                     /label=AAV2 ITR
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa aaacgcgtat tagcttccgc tagacaaatg tgattacccc
     2701 cgctagacaa atgtgattac ccgcgctaga caaatgtgat taccccgcta gacaaatgtg
     2761 attacccccc gctagacaaa tgtgattacc cccgctagac aaatgtgatt acccgcgcta
     2821 gacaaatgtg attaccccgc tagacaaatg tgattacccc cgaccagggc ataaatggcc
     2881 aggtgggacc agagatatcg ctagcaagga tccatggcat cacaggtcgt ctatggggaa
     2941 tgggcatcaa ctcacaccga gtgctacaat atgtccagaa tcgactccac cttcgtctca
     3001 ctgctgcagc tggtgtgggc cgtggtctca ggctgccaga caatcttcat gattagcagg
     3061 gctcccaagg tcccttggga gtccgtgtac ctgccctttg tcgaatctat cacttatgca
     3121 ctggccagta ctggcaacgg aaccctgcag atgagggacg gcagattctt tccttggagc
     3181 cgaatggcct cctggctgtg cacctgtcca atcatgctgg gacagatttc taacatggct
     3241 ctggtgaagt acaaaagtat cccactgaat cccattgcac aggccgcttc catcattaga
     3301 gtggtcatgg gaatcaccgc tacaatttct cccgcagagt atatgaagtg gctgttcttt
     3361 ttctttgggg ccacctgcct ggtgttcgag tacagtgtgg tcttcacaat ctttcaagtg
     3421 gggctgtatg gctttgaatc tgtcgggaca cctctggcac agaaggtggt ggtgaggatc
     3481 aagatgctgc gcctgatctt ctttattgct tggactatgt tcccaatcgt gtggctgatt
     3541 tcacccaccg gagtctgcgt gatccacgag aacgtgagcg ccattctgta cctgctggct
     3601 gacggcctgt gcaaaaatac atatggagtg atcctgtgga gcactgcctg gggagtgctg
     3661 gaagggaagt gggaccccgc atgtctgcca gggcaggaga aacccgaagc cgacgatcct
     3721 tttggcctga atcacgagaa gaatgcccct cctaacgacg aagtcaatat cagaatgttt
     3781 ggaaggggac gtacggcggc caagagcagg atcaccagcg agggcgagta catccccctg
     3841 gaccagatcg acatcaacgt ggcggtaccc gtgagcaagg gcgaggagct gttcaccggg
     3901 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3961 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4021 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgagctgggg cgtgcagtgc
     4081 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4141 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4201 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4261 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcca cggcaacgtc
     4321 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcggcct caactgcaac
     4381 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4441 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     4501 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4561 ctcggcatgg acgagctgta taccggtgca gccctgcagg agaagaagtc atgcagccag
     4621 cgcatggccg aattccggca atactgttgg aacccggaca ctgggcagat gctgggccgc
     4681 accccagccc ggtgggtgtg gatcagcctg tactatgcag ctttctacgt ggtcatgact
     4741 gggctctttg ccttgtgcat ctatgtgctg atgcagacca ttgatcccta cacccccgac
     4801 taccaggacc agttaaagtc accgggggta accttgagac cggatgtgta tggggaaaga
     4861 gggctgcaga tttcctacaa catctctgaa aacagctcta gacaggccca gatcaccgga
     4921 cgtccggagg gagctgtaca agagctgatc agcagcgcca ccagatctct gtttgccgcc
     4981 ggaggcatca acccttggcc taacccctac caccacgagg acatgggctg tggaggaatg
     5041 acacctacag gcgagtgctt cagcaccgag tggtggtgtg acccttctta cggactgagc
     5101 gacgccggat acggatattg cttcgtggag gccacaggcg gctacctggt cgtgggagtg
     5161 gagaagaagc aggcttggct gcacagcaga ggcacaccag gagaaaagat cggcgcccag
     5221 gtctgccagt ggattgcttt cagcatcgcc atcgccctgc tgacattcta cggcttcagc
     5281 gcttggaagg ccacttgcgg ttgggaggag gtctacgtct gttgcgtcga ggtgctgttc
     5341 gtgaccctgg agatcttcaa ggagttcagc agccccgcca cagtgtacct gtctaccggc
     5401 aaccacgcct attgcctgcg ctacttcgag tggctgctgt cttgccccgt gatcctgatc
     5461 aagctgagca acctgagcgg cctgaagaac gactacagca agcggaccat gggcctgatc
     5521 gtgtcttgcg tgggaatgat cgtgttcggc atggccgcag gactggctac cgattggctc
     5581 aagtggctgc tgtatatcgt gtcttgcatc tacggcggct acatgtactt ccaggccgcc
     5641 aagtgctacg tggaagccaa ccacagcgtg cctaaaggcc attgccgcat ggtcgtgaag
     5701 ctgatggcct acgcttactt cgcctcttgg ggcagctacc caatcctctg ggcagtggga
     5761 ccagaaggac tgctgaagct gagcccttac gccaacagca tcggccacag catctgcgac
     5821 atcatcgcca aggagttttg gaccttcctg gcccaccacc tgaggatcaa gatccacgag
     5881 cacatcctga tccacggcga catccggaag accaccaaga tggagatcgg aggcgaggag
     5941 gtggaagtgg aagagttcgt ggaggaggag gacgaggaca cagtgctcga gcagagccag
     6001 cccatcctga acaccaagga gatggccccc cagagcaagc cccccgagga gctggagatg
     6061 agcagcatgc ccagccccgt ggcccccctg cccgcccgca ccgagggcgt gatcgacatg
     6121 cgcagcatga gcagcatcga cagcttcatc agctgcgcca ccgacttccc cgaggccacc
     6181 cgcttctgat ctagaaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     6241 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6301 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6361 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6421 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6481 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6541 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6601 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6661 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6721 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6781 tgggccgcct ccccgcatcg ataccgtcga cccgggcggc cgctcataaa gctcgactgt
     6841 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6901 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6961 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7021 agacaatagc aggcatgctg gggatgcggt ccggactgta caattgcatt cattttatgt
     7081 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     7141 gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta
     7201 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     7261 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     7321 tcagtgagcg agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7381 gtttgcgtat tgggcgct
//
