LOCUS       sequence-379296-        6969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of excitatory 
            neurons with blue shifted excitation spectrum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CaMKII-somBiPOLES-ChroME2s-mCerulean
FEATURES             Location/Qualifiers
     source          1..6969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(1527..1548)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1788..1807)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2135..2156
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3744..4147
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    4154..4202
                     /note="synthetic polyadenylation signal"
     repeat_region   4232..4372
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4232..4361
                     /label=AAV2 ITR
     rep_origin      4447..4902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4534..4553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4744..4765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4919..4938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5038..5060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5098..5116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5184..5288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5289..5357,5358..6149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5507..5526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6320..6908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6809..6828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcttgtg gactaagttt gttcgcatcc ccttctccaa
      181 ccccctcagt acatcaccct gggggaacag ggtccacttg ctcctgggcc cacacagtcc
      241 tgcagtattg tgtatataag gccagggcaa agaggagcag gttttaaagt gaaaggcagg
      301 caggtgttgg ggaggcagtt accggggcaa cgggaacagg gcgtttcgga ggtggttgcc
      361 atggggacct ggatgctgac gaaggctcgc gaggctgtga gcagccacag tgccctgctc
      421 agaagcccca agctcgtcag tcaagccggt tctccgtttg cactcaggag cacgggcagg
      481 cgagtggccc ctagttctgg gggcagctct agagcggtac cggatccatg gcatcacagg
      541 tcgtctatgg ggaatgggca tcaactcaca ccgagtgcta caatatgtcc agaatcgact
      601 ccaccttcgt ctcactgctg cagctggtgt gggccgtggt ctcaggctgc cagacaatct
      661 tcatgattag cagggctccc aaggtccctt gggagtccgt gtacctgccc tttgtcgaat
      721 ctatcactta tgcactggcc agtactggca acggaaccct gcagatgagg gacggcagat
      781 tctttccttg gagccgaatg gcctcctggc tgtgcacctg tccaatcatg ctgggacaga
      841 tttctaacat ggctctggtg aagtacaaaa gtatcccact gaatcccatt gcacaggccg
      901 cttccatcat tagagtggtc atgggaatca ccgctacaat ttctcccgca gagtatatga
      961 agtggctgtt ctttttcttt ggggccacct gcctggtgtt cgagtacagt gtggtcttca
     1021 caatctttca agtggggctg tatggctttg aatctgtcgg gacacctctg gcacagaagg
     1081 tggtggtgag gatcaagatg ctgcgcctga tcttctttat tgcttggact atgttcccaa
     1141 tcgtgtggct gatttcaccc accggagtct gcgtgatcca cgagaacgtg agcgccattc
     1201 tgtacctgct ggctgacggc ctgtgcaaaa atacatatgg agtgatcctg tggagcactg
     1261 cctggggagt gctggaaggg aagtgggacc ccgcatgtct gccagggcag gagaaacccg
     1321 aagccgacga tccttttggc ctgaatcacg agaagaatgc ccctcctaac gacgaagtca
     1381 atatcagaat gtttggaagg ggacgtacgg cggccaagag caggatcacc agcgagggcg
     1441 agtacatccc cctggaccag atcgacatca acgtggcggt acccgtgagc aagggcgagg
     1501 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1561 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1621 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagct
     1681 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1741 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1801 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1861 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
     1921 tccacggcaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttcg
     1981 gcctcaactg caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2041 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     2101 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2161 ccgccgggat cactctcggc atggacgagc tgtataccgg tgcagccctg caggagaaga
     2221 agtcatgcag ccagcgcatg gccgaattcc ggcaatactg ttggaacccg gacactgggc
     2281 agatgctggg ccgcacccca gcccggtggg tgtggatcag cctgtactat gcagctttct
     2341 acgtggtcat gactgggctc tttgccttgt gcatctatgt gctgatgcag accattgatc
     2401 cctacacccc cgactaccag gaccagttaa agtcaccggg ggtaaccttg agaccggatg
     2461 tgtatgggga aagagggctg cagatttcct acaacatctc tgaaaacagc tctagacagg
     2521 cccagatcac cggacgtccg gagggagctg tacaagaaac agccgccaca atgacccacg
     2581 cctttatctc agccgtgcct agcgccgaag ccacaattag aggcctgctg agcgccgcag
     2641 cagtggtgac accagcagca gacgctcacg gagaaacctc taacgccaca acagccggag
     2701 ccgatcacgg ttgcttcccc cacatcaacc acggaaccga gctgcagcac aagatcgcag
     2761 tgggactcca gtggttcacc gtgatcgtgg ctatcgtgca gctcatcttc tacggttggc
     2821 acagcttcaa ggccacaacc ggctgggagg aggtctacgt ctgcgtgatc gagctcgtca
     2881 agtgcttcat cgagctgttc cacgaggtcg acagcccagc cacagtgtac cagaccaacg
     2941 gaggagccgt gatttggctg cggtacagcg agtggctcct gacttgcccc gtgatcctga
     3001 tccacctgag caacctgacc ggactgcacg aagagtacag caagcggacc atgaccatcc
     3061 tggtgaccga catcggcaac atcgtgtggg ggatcacagc cgcctttaca aagggccccc
     3121 tgaagatcct gttcttcatg atcggcctgt tctacggcgt gacttgcttc ttccagatcg
     3181 ccaaggtgta tatcgagagc taccacaccc tgcccaaagg cgtctgccgg aagatttgca
     3241 agatcatggc ctacgtcttc ttctgctctt ggctgatgtt ccccgtgatg ttcatcgccg
     3301 gacacgaggg actgggcctg atcacacctt acaccagcgg aatcggccac ctgatcctgg
     3361 atctgatcgc caagaacact tggggcttcc tgggccacca cctgagagtg aagatccacg
     3421 agcacatcct gatccacggc gacatccgga agacaaccac catcaacgtg gccggcgaga
     3481 acatggagat cgagaccttc gtcgacgagg aggaggaggg aggagtgctc gagcagagcc
     3541 agcccatcct gaacaccaag gagatggccc cccagagcaa gccccccgag gagctggaga
     3601 tgagcagcat gcccagcccc gtggcccccc tgcccgcccg caccgagggc gtgatcgaca
     3661 tgcgcagcat gagcagcatc gacagcttca tcagctgcgc caccgacttc cccgaggcca
     3721 cccgcttctg atctagaaag cttaatcaac ctctggatta caaaatttgt gaaagattga
     3781 ctgatattct taactatgtt gctcctttta cgctgtgtgg atatgctgct ttaatgcctc
     3841 tgtatcatgc tattgcttcc cgtatggctt tcgttttctc ctccttgtat aaatcctggt
     3901 tgctgtctct ttatgaggag ttgtggcccg ttgtccgtca acgtggcgtg gtgtgctctg
     3961 tgtttgctga cgcaaccccc actggctggg gcattgccac cacctgtcaa ctcctttctg
     4021 ggactttcgc tttccccctc ccgatcgcca cggcagaact catcgccgcc tgccttgccc
     4081 gctgctggac aggggctagg ttgctgggca ctgataattc cgtggtgttg tcggggaagc
     4141 tgacgtcagc gctaataaaa tcgctatcca tcgaagatgg atgtgtgttg gttttttgtg
     4201 tgggtaaccc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4261 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4321 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4381 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4441 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4501 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4621 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4741 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     4801 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     4861 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     4921 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4981 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5041 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5101 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5161 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5221 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5281 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5341 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5401 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5461 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5521 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5581 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5641 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5701 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5761 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5821 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5881 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5941 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6001 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6061 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6121 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6181 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6241 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6301 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6361 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6421 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6481 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6541 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6601 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6661 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6721 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6781 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6841 ggttttgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6901 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6961 ctcacatgt
//
