LOCUS       sequence-379309-       12605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TAOK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12605)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TAOK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..12605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8584..8608)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8610..8651
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8658..9428
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(9434..9454)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    9483..10071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9536..9556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9954..9965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10085..10584
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10120..10143)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10517..10536
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10605..11204
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10605..10624)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11101..11121
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11342..11575
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11647..11768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11684..11703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11738..11757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11808..11943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11963..11982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11964..11982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11992..12009)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11992..12008)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12001..12023)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12150..12578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12237..12256)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12447..12468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatgccagc tgggggccgg gccgggagcc
     5461 tgaaggaccc agatgtggct gagctcttct tcaaggatga cccagaaaag ctcttctctg
     5521 acctccggga aattggccat ggcagctttg gagccgtata ctttgcccgg gatgtccgga
     5581 atagtgaggt ggtggccatc aagaagatgt cctacagtgg gaagcagtcc aatgagaaat
     5641 ggcaagacat catcaaggag gtgcggttct tacagaagct ccggcatccc aacaccattc
     5701 agtaccgggg ctgttacctg agggagcaca cggcttggct ggtaatggag tattgcctgg
     5761 gctcagcttc tgaccttcta gaagtgcaca agaaacccct tcaggaggta gagatcgcag
     5821 ctgtgaccca cggggcgctt cagggcctgg catatctgca ctcccacaac atgatccata
     5881 gggatgtgaa ggctggaaac atcctgctgt cagagccagg gttagtgaag ctaggggact
     5941 ttggttctgc gtccatcatg gcacctgcca actccttcgt gggcacccca tactggatgg
     6001 cacccgaggt gatcctggcc atggatgagg ggcagtacga tggcaaagtg gacgtctggt
     6061 ccttggggat aacctgcatc gagctggctg aacggaaacc accgctcttt aacatgaatg
     6121 cgatgagtgc cttataccac attgcacaga acgaatcccc cgtgctccag tcaggacact
     6181 ggtctgagta cttccggaat tttgtcgact cctgtcttca gaaaatccct caagacagac
     6241 caacctcaga ggttctcctg aagcaccgct ttgtgctccg ggagcggcca cccacagtca
     6301 tcatggacct gatccagagg accaaggatg ccgtgcggga gctggacaac ctgcagtacc
     6361 gcaagatgaa gaagatcctg ttccaagagg cacccaacgg ccctggtgcc gaggccccag
     6421 aggaggaaga ggaggccgag ccctacatgc accgggccgg gactctgacc agcctcgaga
     6481 gtagccactc agtgcccagc atgtccatca gcgcctccag ccagagcagc tccgtcaaca
     6541 gcctagcaga tgcctcagac aacgaggaag aggaggagga ggaggaggaa gaggaggagg
     6601 aggaagaagg ccctgaagcc cgggagatgg ccatgatgca ggagggggag cacacagtca
     6661 cctctcacag ctccattatc caccggctgc cgggctctga caacctatat gatgacccct
     6721 accagccaga gataaccccc agccctctcc agccgcctgc agccccagct cccacttcca
     6781 ccacctcttc tgcccgccgc cgggcctact gccgtaaccg agaccacttt gccaccatcc
     6841 gaaccgcctc cctggtcagc cgtcagatcc aggagcatga gcaggactct gcgctgcggg
     6901 agcagctgag cggctataag cggatgcgac gacagcacca gaagcagctg ctggccctgg
     6961 agtcacggct gaggggtgaa cgggaggagc acagtgcacg gctgcagcgg gagcttgagg
     7021 cgcagcgggc tggctttggg gcagaggcag aaaagctggc ccggcggcac caggccatag
     7081 gtgagaagga ggcacgagct gcccaggccg aggagcggaa gttccagcag cacatccttg
     7141 ggcagcagaa gaaggagctg gctgccctgc tggaggcaca gaagcggacc tacaaacttc
     7201 gcaaggaaca gctgaaggag gagctccagg agaaccccag cactcccaag cgggagaagg
     7261 ccgagtggct gctgcggcag aaggagcagc tccagcagtg ccaggcggag gaggaagcag
     7321 ggctgctgcg gcggcagcgc cagtactttg agctgcagtg tcgccagtac aagcgcaaga
     7381 tgttgctggc tcggcacagc ctggaccagg acctgctgcg ggaggacctg aacaagaagc
     7441 agacccagaa ggacttggag tgtgcactgc tgcttcggca gcacgaggcc acgcgggagc
     7501 tggagctgcg gcagctccag gccgtgcagc gcacgcgggc tgagctcacc cgcctgcagc
     7561 accagacgga gctgggcaac cagctggagt acaacaagcg gcgtgagcaa gagttgcggc
     7621 agaagcatgc ggcccaggtt cgccagcagc ccaagagcct caaatctaag gagctgcaga
     7681 tcaagaagca gttccaggag acgtgtaaga tccagactcg gcagtacaag gctctgcgag
     7741 cacacttgct ggagaccacg cccaaagctc agcacaagag cctccttaag cggctcaagg
     7801 aagagcagac ccgcaagctg gcgatcttgg cggagcagta tgaccagtcc atctcagaga
     7861 tgctcagctc acaggcgctg cggcttgatg agacccagga ggcagagttc caggcccttc
     7921 ggcagcagct tcaacaggag ctggagctgc tcaacgctta ccagagcaag atcaagatcc
     7981 gcacagagag ccagcacgag agggagctgc gggagctgga gcagagggtc gcgctgcggc
     8041 gggcactgct ggagcagcgg gtggaagagg agctgctggc cctgcagaca ggacgctccg
     8101 agcgaatccg cagtctgctt gagcggcagg cccgtgagat cgaggccttc gatgcggaaa
     8161 gcatgaggct gggcttctcc agcatggctc tggggggcat cccggctgaa gctgctgccc
     8221 agggctatcc tgctccaccc cctgccccag cctggccctc ccgtcccgtt ccccgttctg
     8281 gggcacactg gagccatggc cctcctccac caggcatgcc ccctccagcc tggcgtcagc
     8341 cgtctctgct ggctccccca ggccccccaa actggctggg gccccccaca caaagtggga
     8401 caccccgtgg cggagccctg ctgctgctaa gaaacagccc ccagcccctg cggcgggcag
     8461 cctcgggggg cagtggcagt gagaatgtgg gcccccctgc tgccgcggtg cccgggcccc
     8521 tgagccgcag caccagtgtc gcttcccaca tcctcaatgg ttcttcccac ttctattcct
     8581 caaacccagc tttcttgtac aaagtgggcg gcaagcccat ccccaacccc ctgctgggcc
     8641 tggacagcac cgctagcatc ccgctgctga acgctaaaca gattctggga cagctggacg
     8701 gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg ctggatcgaa
     8761 tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca gggagaggaa
     8821 gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt atgttttggc
     8881 gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt gtcatggcag
     8941 aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat gacctgtatc
     9001 tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg
     9061 cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg
     9121 tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg
     9181 ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg
     9241 ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga
     9301 tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc
     9361 tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg
     9421 ccgaaaagta agccaacttt attatacata gttgatatcc agcacagtgg cggccgctcg
     9481 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9841 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     9901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
    10081 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
    10141 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
    10201 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
    10261 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
    10321 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
    10381 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
    10441 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
    10501 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
    10561 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
    10621 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    10681 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
    10741 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    10801 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    10861 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    10921 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    10981 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    11041 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    11101 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    11161 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
    11221 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
    11281 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    11341 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    11401 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    11461 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    11521 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    11581 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    11641 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    11701 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11761 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
    11821 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    11881 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11941 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    12001 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    12061 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    12121 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
    12181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    12241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    12301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    12361 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    12421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    12481 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    12541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    12601 aattt
//
