LOCUS       sequence-379322-       10731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mCherry
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..34
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(72..94)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     194..213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        237..452
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             453..1256
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1387..1842)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1524..1545)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1736..1755
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             4769..4780
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5269..5979
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5414..5432)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5682..5701
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7224..7246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7235..7251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7259..7265,7266..7283,7284..7289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7304..7325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7442..7459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7613..8201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7693..7712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8372..9163,9164..9232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8995..9014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9233..9337)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9364..10706
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10327..10374)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatac caccttttca
      241 attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt
      301 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
      361 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
      421 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
      481 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
      541 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
      601 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
      661 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
      721 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
      781 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
      841 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
      901 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
      961 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     1021 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     1081 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     1141 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     1201 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     1261 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     1321 atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     1381 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1441 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1501 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     1561 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     1621 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1681 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1741 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1801 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     1861 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     1921 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     1981 ctggagaagc tcattccacc cgaaatgccc gtgaacttgc ccttgtttgc gcgggccttc
     2041 ttgcgctctg ctcggatacg ctcgacatcg ctgaggagct cggcgagttc cttggcgcgg
     2101 ttgcggacgt tgataccctg gtctttgccg ttctggtcga taaaatggaa ctggcgcagc
     2161 atcttgagga gggtaagatg cgaccgagca tcgtccacca ctcgttcgga accgtgctta
     2221 atcaggtact ctagtagttg aagggccttg tagatctggc gccattcttc ggcggacttt
     2281 tctgtgaaac ggcggtaaat catgggcatg atttcgttga gtgtttggct aagaacaagg
     2341 agggaaacgc aagttagccg cgttcgtata caagcgagca gttctcgaga acgtactagt
     2401 tgaaggtgcc atcggcgatc tcttgcatca aagtcgacga tgcaccccaa ggttcattgt
     2461 tggtggcctc gcgcacctgg gttcgctgtc agctcccgtt ccatggtctc ttgtctggga
     2521 tcaagtatat ggcgaagggc gcaagagaat ggtgccagtg tggcgcgtgg atacgggcat
     2581 accttggact ccatctcggt atagttcatg acagctggtt ggagccattg tcagcaccct
     2641 gaacacgaaa tcatgaggga gtcttggttc tggactaggt ggtcacctac cattttgaac
     2701 ctttcgtacg ccagccttga tgtcgtagag tgtcaggttg gacacggtat ccttgaggct
     2761 attgaaatcc attgcgtaaa cgtgtctatc gccggagttg ctgggaattg ttctgatggg
     2821 ctaaggtaat tcttctctag atccaatctg agcctcgcgg tgatgattgc cagacgggcg
     2881 ggatgctgtg gcgacttggt ggtttttgtt cgggctgatc gctttcaggg cgacgtttca
     2941 cggtcttgtc gggatcagat ggacattgag gtgcggaatt atacgattta ggtgactgca
     3001 ggccatacag atctccctag acgccgggcc aatccgttcc ttgcaccttc tctcacacat
     3061 gcaggttttg gaatgtggct tcatcgtgtc cagaactggg cgcattgggg ccaggctcag
     3121 tgtcagtgta gggatcgatg tacatatggc agttgacagc gagagagcca ggtgtcacag
     3181 gcagtccgtc agtggccgcg gtggtggcca cgcactttct gcaggagccg tctggggtgc
     3241 gtgcagctgg cggccgcttc cgggcatacg gcttgtgtaa cgagggtgtt gaccctcggc
     3301 ttggcgcttg aacttggcgc tgcggtcccc ccacagttga ccacaatccc cggccaatcg
     3361 aacgacggca acaagggcgc actagccagt gaacgcctgg ggatagtggg tggtcaaaga
     3421 gaattgggca aacgagacga caatggaggg tgaaccggga ataataaaca gctggtgaga
     3481 ccacccatca aacgaatcac cccaccatgg gatgccctca gatgccctcc atgccaccat
     3541 aaggaatgat cttacggctt gccgaagtta acaggcctgc ttttgcatat gaacgagagc
     3601 ctggtgttgc attccacatt ggctatctac agtacctacc caacctacac accctgatgc
     3661 gaaagggaca gaaggggaga aggatacata catatacctg actcgtgccc atcaacaaca
     3721 gagaaagggt gatcgctgaa cacatcagag catctcttcc cccctggcga ctaaaagtgc
     3781 tcccaatctc gtcttacatc tgatacgttg ttcaaactcc gcaacccgat ctgcacactg
     3841 ccctgccttg gcccttcttg gagtctccgc ttccccacgc catctcacgc tcgaggactc
     3901 cgctgctagg tatgagctag gcctgccgtc gacaggatcc accagacgtc aacaataacc
     3961 catcttggaa atacataaga agacgtcact tccctccctt ttcgaaagga agagatcatc
     4021 atcagcatcc atcatggctg gtctttttgg ttccgccgcc gcatccgcga gcaacaccct
     4081 cggcgatctt aagcaggatg tcgagctcgg ccaacccccc gaggacagca tcagcgacct
     4141 tgccttcaac cccaaccccg ccgaccagaa ggacttcctc gccgtagcca gctgggacaa
     4201 gaagacacgc atttacgaga tcttgagtaa tggtcaaggt cagggacagg ccatgattga
     4261 gcatgatgcc cccgttttct cttgcgattt cttcaaggta tgtttgcgtc gattggcctg
     4321 gcaggtagcc cgacaaaaca ctgacatgca cttctcccaa caggacggca caaaggtcat
     4381 ttccgccggc gccgacaagg ccgccaaggt tctcgatttg gccaccggtc agtcgatgca
     4441 ggtcgctgct cacgatatgc ccatcaagtg cgttcgctac ttcgaggcca atggcacgcc
     4501 catggcggtg acaggtggct gggacaagca gatcaagtac tgggatttcc gctccgcgaa
     4561 cccggccgcc acggtgcagg cccaggagcg cgtttatacc atggatgtgc gcgacaactt
     4621 gctcgtggtc ggaaccgctg atcgctatat caacgtgatc aacctgaagg accccggcaa
     4681 gttctacaag acaatgcaga gccctcttaa atggcagact cgtgtggtca gctgcttcaa
     4741 cgattctcag ggctttgcta tcggctcgat cgagggtaga tgcgccattc aatatgtgga
     4801 ggacaaggat tctgcgtacg ccttcacctc ctcccccttt actatcccat ctgccgaata
     4861 cgctgactaa ctgacttgtt ttccagctcc aacttctctt tcaaatgcca ccgcgacccc
     4921 gcccaaggca acaccacggc cgtccacgct gtcaacgaca tctccttcca cccccaacac
     4981 ggcaccttca gcacagccgg tagcgacggc accttccact tctgggacaa ggacgccaag
     5041 caccgtctca agggttaccc caacgtcggc ggcagcatca cctcgacaac gttcaacaag
     5101 accggcagca tctttgccta cgccattagt tatgactgga gcaaggggta ccaaggcaac
     5161 tcgcccacgt acccgaccaa ggtcatgctc cacccagtac agcaggacga gtgcaagccc
     5221 cgtccgagcg ttaagaagag ggctggtatt ggtttggcta ttgctggcat ggtgagcaag
     5281 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     5341 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     5401 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5461 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     5521 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     5581 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     5641 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     5701 acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     5761 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     5821 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     5881 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     5941 cgccactcca ccggcggcat ggacgagctg tacaagtgac tagaggatcc gacactatag
     6001 aatacctaga cagtttgcag gtcatgtgaa tgttatcgcc aacacattga atgctaaaca
     6061 gattacctcc aacgcctaga tgaaaccaaa ttacccttgc cttctttttt tttacgccgt
     6121 taatgcagtt gtgatagaga ccatgcaacg tcttctaaac ggagacacaa acacattcga
     6181 gtcgatcaga tcgaaatgac ttgttcctgt acctcgtcac catccagcca ccgtctgaaa
     6241 tagacgcagt tgctggggct gacaggtccg ccttcctcgc acatatcata gacagggcac
     6301 cgcccacaag gcgcctccat aaggccactt gcactcggct caactcccat aaatgaggta
     6361 acaatgcctc ctttggttgg atcatggtcg aggcgtcgtt gattgcacgt cctcggatcc
     6421 tgtttgggga tgcgcgtcac acggtacccc ttgcgcccat tgaccttgac aggctctact
     6481 aagccatcgt agtatagcac atccaccaac tgctggatat ccgcctcgct caacgtcgta
     6541 ttattcgtga tgccggcgtg gtggatgaac tttgcaatgt ccgacacagt cgggtacgac
     6601 ttgtaaccgg ccgggagagg cagcaattgc ggcttgccgg gcctggtctt cttggccggt
     6661 gccggtgccg ccgtgtcgtc gttggaaatc tcgcccgcgc tgcgcttcgt ccccttggaa
     6721 atatcctccg agtggggaat ggtcccctga accacgccct tcttgggatt cttttcacgc
     6781 tgctgtgctc ctcctccttg tgctcctact cctggtactc cagcaccgcc gccgtgggaa
     6841 gtatatgtcg atttctgctt gataaagtca aacacaattt cctgtagctc gttgatgaag
     6901 gccgtgtcga gctcgccctc agtaaaccag gggccacctg tggcgcgatc ggagggacgt
     6961 aggttcgcct tgatgtacat cttcttgttg gggtgctcga cgttcttcat gctggcgatg
     7021 tagccctttt gctcgagata cttgatgcaa tgcttcataa gcgagtcggc catgttgagc
     7081 ttgttcttga tggtccggtt ccagatgccg tcggccccgg cttcgtcgac catggcgtat
     7141 accatcatta tttcggagtt ggggagggag gtgtaccttt tgggaggaag gggaggttag
     7201 ttggtgtgct cggttggctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7261 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7321 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7381 attaatgaat cggccaacgc gcggggagag gcggtttgtg tattgggcgc tcttccgctt
     7441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9361 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     9421 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     9481 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     9541 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     9601 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     9661 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     9721 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     9781 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     9841 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     9901 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     9961 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
    10021 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
    10081 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
    10141 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
    10201 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
    10261 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
    10321 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
    10381 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
    10441 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
    10501 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
    10561 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
    10621 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
    10681 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat g
//
