LOCUS       sequence-379323-       10731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mNeonGreen
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1952..3294
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2915..2962)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3335..3353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3391..3413)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3513..3532
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3556..3771
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3772..4575
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(4706..5161)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4843..4864)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5055..5074
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             8088..8099
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      8843..8852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(10543..10565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10554..10570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10578..10584,10585..10602,10603..10608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10623..10644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgtgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga
     1981 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     2041 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     2101 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     2161 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     2221 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     2281 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     2341 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     2401 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     2461 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     2521 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     2581 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     2641 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     2701 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     2761 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     2821 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     2881 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
     2941 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     3001 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     3061 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
     3121 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
     3181 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
     3241 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta
     3301 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3361 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3421 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3481 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3541 gcaccatacc accttttcaa ttcatcattt tttttttatt cttttttttg atttcggttt
     3601 ccttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac
     3661 agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta
     3721 ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa
     3781 gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat
     3841 atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa
     3901 ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat
     3961 atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag
     4021 tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg
     4081 cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt
     4141 gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa
     4201 cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa
     4261 tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt
     4321 gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt
     4381 gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg
     4441 gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat
     4501 gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc
     4561 ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt
     4621 agagcttcaa tttaattata tcagttatta ccctatgcgg tgtgaaatac cgcacagatg
     4681 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     4741 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4861 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4921 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     4981 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5041 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5101 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5161 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     5281 ggttttccca gtcacgacgc tggagaagct cattccaccc gaaatgcccg tgaacttgcc
     5341 cttgtttgcg cgggccttct tgcgctctgc tcggatacgc tcgacatcgc tgaggagctc
     5401 ggcgagttcc ttggcgcggt tgcggacgtt gataccctgg tctttgccgt tctggtcgat
     5461 aaaatggaac tggcgcagca tcttgaggag ggtaagatgc gaccgagcat cgtccaccac
     5521 tcgttcggaa ccgtgcttaa tcaggtactc tagtagttga agggccttgt agatctggcg
     5581 ccattcttcg gcggactttt ctgtgaaacg gcggtaaatc atgggcatga tttcgttgag
     5641 tgtttggcta agaacaagga gggaaacgca agttagccgc gttcgtatac aagcgagcag
     5701 ttctcgagaa cgtactagtt gaaggtgcca tcggcgatct cttgcatcaa agtcgacgat
     5761 gcaccccaag gttcattgtt ggtggcctcg cgcacctggg ttcgctgtca gctcccgttc
     5821 catggtctct tgtctgggat caagtatatg gcgaagggcg caagagaatg gtgccagtgt
     5881 ggcgcgtgga tacgggcata ccttggactc catctcggta tagttcatga cagctggttg
     5941 gagccattgt cagcaccctg aacacgaaat catgagggag tcttggttct ggactaggtg
     6001 gtcacctacc attttgaacc tttcgtacgc cagccttgat gtcgtagagt gtcaggttgg
     6061 acacggtatc cttgaggcta ttgaaatcca ttgcgtaaac gtgtctatcg ccggagttgc
     6121 tgggaattgt tctgatgggc taaggtaatt cttctctaga tccaatctga gcctcgcggt
     6181 gatgattgcc agacgggcgg gatgctgtgg cgacttggtg gtttttgttc gggctgatcg
     6241 ctttcagggc gacgtttcac ggtcttgtcg ggatcagatg gacattgagg tgcggaatta
     6301 tacgatttag gtgactgcag gccatacaga tctccctaga cgccgggcca atccgttcct
     6361 tgcaccttct ctcacacatg caggttttgg aatgtggctt catcgtgtcc agaactgggc
     6421 gcattggggc caggctcagt gtcagtgtag ggatcgatgt acatatggca gttgacagcg
     6481 agagagccag gtgtcacagg cagtccgtca gtggccgcgg tggtggccac gcactttctg
     6541 caggagccgt ctggggtgcg tgcagctggc ggccgcttcc gggcatacgg cttgtgtaac
     6601 gagggtgttg accctcggct tggcgcttga acttggcgct gcggtccccc cacagttgac
     6661 cacaatcccc ggccaatcga acgacggcaa caagggcgca ctagccagtg aacgcctggg
     6721 gatagtgggt ggtcaaagag aattgggcaa acgagacgac aatggagggt gaaccgggaa
     6781 taataaacag ctggtgagac cacccatcaa acgaatcacc ccaccatggg atgccctcag
     6841 atgccctcca tgccaccata aggaatgatc ttacggcttg ccgaagttaa caggcctgct
     6901 tttgcatatg aacgagagcc tggtgttgca ttccacattg gctatctaca gtacctaccc
     6961 aacctacaca ccctgatgcg aaagggacag aaggggagaa ggatacatac atatacctga
     7021 ctcgtgccca tcaacaacag agaaagggtg atcgctgaac acatcagagc atctcttccc
     7081 ccctggcgac taaaagtgct cccaatctcg tcttacatct gatacgttgt tcaaactccg
     7141 caacccgatc tgcacactgc cctgccttgg cccttcttgg agtctccgct tccccacgcc
     7201 atctcacgct cgaggactcc gctgctaggt atgagctagg cctgccgtcg acaggatcca
     7261 ccagacgtca acaataaccc atcttggaaa tacataagaa gacgtcactt ccctcccttt
     7321 tcgaaaggaa gagatcatca tcagcatcca tcatggctgg tctttttggt tccgccgccg
     7381 catccgcgag caacaccctc ggcgatctta agcaggatgt cgagctcggc caaccccccg
     7441 aggacagcat cagcgacctt gccttcaacc ccaaccccgc cgaccagaag gacttcctcg
     7501 ccgtagccag ctgggacaag aagacacgca tttacgagat cttgagtaat ggtcaaggtc
     7561 agggacaggc catgattgag catgatgccc ccgttttctc ttgcgatttc ttcaaggtat
     7621 gtttgcgtcg attggcctgg caggtagccc gacaaaacac tgacatgcac ttctcccaac
     7681 aggacggcac aaaggtcatt tccgccggcg ccgacaaggc cgccaaggtt ctcgatttgg
     7741 ccaccggtca gtcgatgcag gtcgctgctc acgatatgcc catcaagtgc gttcgctact
     7801 tcgaggccaa tggcacgccc atggcggtga caggtggctg ggacaagcag atcaagtact
     7861 gggatttccg ctccgcgaac ccggccgcca cggtgcaggc ccaggagcgc gtttatacca
     7921 tggatgtgcg cgacaacttg ctcgtggtcg gaaccgctga tcgctatatc aacgtgatca
     7981 acctgaagga ccccggcaag ttctacaaga caatgcagag ccctcttaaa tggcagactc
     8041 gtgtggtcag ctgcttcaac gattctcagg gctttgctat cggctcgatc gagggtagat
     8101 gcgccattca atatgtggag gacaaggatt ctgcgtacgc cttcacctcc tcccccttta
     8161 ctatcccatc tgccgaatac gctgactaac tgacttgttt tccagctcca acttctcttt
     8221 caaatgccac cgcgaccccg cccaaggcaa caccacggcc gtccacgctg tcaacgacat
     8281 ctccttccac ccccaacacg gcaccttcag cacagccggt agcgacggca ccttccactt
     8341 ctgggacaag gacgccaagc accgtctcaa gggttacccc aacgtcggcg gcagcatcac
     8401 ctcgacaacg ttcaacaaga ccggcagcat ctttgcctac gccattagtt atgactggag
     8461 caaggggtac caaggcaact cgcccacgta cccgaccaag gtcatgctcc acccagtaca
     8521 gcaggacgag tgcaagcccc gtccgagcgt taagaagagg gctggtattg gtttggctat
     8581 tgctggcatg gtctccaagg gcgaggagga caacatggcc tccctccccg ccacccacga
     8641 gctccacatc ttcggctcca tcaacggcgt cgacttcgac atggtcggcc agggcaccgg
     8701 caaccccaac gacggctacg aggagctcaa cctcaagtcc accaagggcg acctccagtt
     8761 ctccccctgg atcctcgtcc cccacatcgg ctacggcttc caccagtacc tcccctaccc
     8821 cgacggcatg tcccccttcc aggccgccat ggtcgacggc tccggctacc aggtccaccg
     8881 caccatgcag ttcgaggacg gcgcctccct caccgtcaac taccgctaca cctacgaggg
     8941 ctcccacatc aagggcgagg cccaggtcaa gggcaccggc ttccccgccg acggccccgt
     9001 catgaccaac tccctcaccg ccgccgactg gtgccgctcc aagaagacct accccaacga
     9061 caagaccatc atctccacct tcaagtggtc ctacaccacc ggcaacggca agcgctaccg
     9121 ctccaccgcc cgcaccacct acaccttcgc caagcccatg gccgccaact acctcaagaa
     9181 ccagcccatg tacgtcttcc gcaagaccga gctcaagcac tccaagaccg agctcaactt
     9241 caaggagtgg cagaaggcct tcaccgacgt catgggcatg gacgagctct acaagtgact
     9301 agaggatccg acactataga atacctagac agtttgcagg tcatgtgaat gttatcgcca
     9361 acacattgaa tgctaaacag attacctcca acgcctagat gaaaccaaat tacccttgcc
     9421 ttcttttttt ttacgccgtt aatgcagttg tgatagagac catgcaacgt cttctaaacg
     9481 gagacacaaa cacattcgag tcgatcagat cgaaatgact tgttcctgta cctcgtcacc
     9541 atccagccac cgtctgaaat agacgcagtt gctggggctg acaggtccgc cttcctcgca
     9601 catatcatag acagggcacc gcccacaagg cgcctccata aggccacttg cactcggctc
     9661 aactcccata aatgaggtaa caatgcctcc tttggttgga tcatggtcga ggcgtcgttg
     9721 attgcacgtc ctcggatcct gtttggggat gcgcgtcaca cggtacccct tgcgcccatt
     9781 gaccttgaca ggctctacta agccatcgta gtatagcaca tccaccaact gctggatatc
     9841 cgcctcgctc aacgtcgtat tattcgtgat gccggcgtgg tggatgaact ttgcaatgtc
     9901 cgacacagtc gggtacgact tgtaaccggc cgggagaggc agcaattgcg gcttgccggg
     9961 cctggtcttc ttggccggtg ccggtgccgc cgtgtcgtcg ttggaaatct cgcccgcgct
    10021 gcgcttcgtc cccttggaaa tatcctccga gtggggaatg gtcccctgaa ccacgccctt
    10081 cttgggattc ttttcacgct gctgtgctcc tcctccttgt gctcctactc ctggtactcc
    10141 agcaccgccg ccgtgggaag tatatgtcga tttctgcttg ataaagtcaa acacaatttc
    10201 ctgtagctcg ttgatgaagg ccgtgtcgag ctcgccctca gtaaaccagg ggccacctgt
    10261 ggcgcgatcg gagggacgta ggttcgcctt gatgtacatc ttcttgttgg ggtgctcgac
    10321 gttcttcatg ctggcgatgt agcccttttg ctcgagatac ttgatgcaat gcttcataag
    10381 cgagtcggcc atgttgagct tgttcttgat ggtccggttc cagatgccgt cggccccggc
    10441 ttcgtcgacc atggcgtata ccatcattat ttcggagttg gggagggagg tgtacctttt
    10501 gggaggaagg ggaggttagt tggtgtgctc ggttggctgt ttcctgtgtg aaattgttat
    10561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg c
//
