LOCUS       sequence-379324-       10734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mRuby3
FEATURES             Location/Qualifiers
     source          1..10734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      94..1436
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(1057..1104)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1477..1495
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1533..1555)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1655..1674
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1698..1913
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1914..2717
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(2848..3303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2985..3006)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3197..3216
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             6230..6241
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6730..7440
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     primer_bind     complement(8688..8710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8699..8715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8723..8729,8730..8747,8748..8753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8768..8789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8906..8923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9077..9665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9157..9176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9836..10627,10628..10696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10459..10478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10697..10734,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta
      121 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
      181 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
      241 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
      301 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
      361 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
      421 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
      481 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
      541 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
      601 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
      661 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
      721 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
      781 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
      841 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
      901 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
      961 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
     1021 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa
     1081 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca
     1141 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg
     1201 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat
     1261 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc
     1321 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac
     1381 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac
     1441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     1501 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     1561 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1621 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1681 gtgcaccata ccaccttttc aattcatcat ttttttttta ttcttttttt tgatttcggt
     1741 ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     1801 acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     1861 tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga
     1921 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     1981 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     2041 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     2101 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     2161 agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
     2221 tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
     2281 gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
     2341 aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
     2401 aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
     2461 ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
     2521 gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
     2581 tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
     2641 atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
     2701 gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
     2761 ttagagcttc aatttaatta tatcagttat taccctatgc ggtgtgaaat accgcacaga
     2821 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     2881 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2941 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3001 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3061 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3121 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3181 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3241 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3301 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3361 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3421 agggttttcc cagtcacgac gctggagaag ctcattccac ccgaaatgcc cgtgaacttg
     3481 cccttgtttg cgcgggcctt cttgcgctct gctcggatac gctcgacatc gctgaggagc
     3541 tcggcgagtt ccttggcgcg gttgcggacg ttgataccct ggtctttgcc gttctggtcg
     3601 ataaaatgga actggcgcag catcttgagg agggtaagat gcgaccgagc atcgtccacc
     3661 actcgttcgg aaccgtgctt aatcaggtac tctagtagtt gaagggcctt gtagatctgg
     3721 cgccattctt cggcggactt ttctgtgaaa cggcggtaaa tcatgggcat gatttcgttg
     3781 agtgtttggc taagaacaag gagggaaacg caagttagcc gcgttcgtat acaagcgagc
     3841 agttctcgag aacgtactag ttgaaggtgc catcggcgat ctcttgcatc aaagtcgacg
     3901 atgcacccca aggttcattg ttggtggcct cgcgcacctg ggttcgctgt cagctcccgt
     3961 tccatggtct cttgtctggg atcaagtata tggcgaaggg cgcaagagaa tggtgccagt
     4021 gtggcgcgtg gatacgggca taccttggac tccatctcgg tatagttcat gacagctggt
     4081 tggagccatt gtcagcaccc tgaacacgaa atcatgaggg agtcttggtt ctggactagg
     4141 tggtcaccta ccattttgaa cctttcgtac gccagccttg atgtcgtaga gtgtcaggtt
     4201 ggacacggta tccttgaggc tattgaaatc cattgcgtaa acgtgtctat cgccggagtt
     4261 gctgggaatt gttctgatgg gctaaggtaa ttcttctcta gatccaatct gagcctcgcg
     4321 gtgatgattg ccagacgggc gggatgctgt ggcgacttgg tggtttttgt tcgggctgat
     4381 cgctttcagg gcgacgtttc acggtcttgt cgggatcaga tggacattga ggtgcggaat
     4441 tatacgattt aggtgactgc aggccataca gatctcccta gacgccgggc caatccgttc
     4501 cttgcacctt ctctcacaca tgcaggtttt ggaatgtggc ttcatcgtgt ccagaactgg
     4561 gcgcattggg gccaggctca gtgtcagtgt agggatcgat gtacatatgg cagttgacag
     4621 cgagagagcc aggtgtcaca ggcagtccgt cagtggccgc ggtggtggcc acgcactttc
     4681 tgcaggagcc gtctggggtg cgtgcagctg gcggccgctt ccgggcatac ggcttgtgta
     4741 acgagggtgt tgaccctcgg cttggcgctt gaacttggcg ctgcggtccc cccacagttg
     4801 accacaatcc ccggccaatc gaacgacggc aacaagggcg cactagccag tgaacgcctg
     4861 gggatagtgg gtggtcaaag agaattgggc aaacgagacg acaatggagg gtgaaccggg
     4921 aataataaac agctggtgag accacccatc aaacgaatca ccccaccatg ggatgccctc
     4981 agatgccctc catgccacca taaggaatga tcttacggct tgccgaagtt aacaggcctg
     5041 cttttgcata tgaacgagag cctggtgttg cattccacat tggctatcta cagtacctac
     5101 ccaacctaca caccctgatg cgaaagggac agaaggggag aaggatacat acatatacct
     5161 gactcgtgcc catcaacaac agagaaaggg tgatcgctga acacatcaga gcatctcttc
     5221 ccccctggcg actaaaagtg ctcccaatct cgtcttacat ctgatacgtt gttcaaactc
     5281 cgcaacccga tctgcacact gccctgcctt ggcccttctt ggagtctccg cttccccacg
     5341 ccatctcacg ctcgaggact ccgctgctag gtatgagcta ggcctgccgt cgacaggatc
     5401 caccagacgt caacaataac ccatcttgga aatacataag aagacgtcac ttccctccct
     5461 tttcgaaagg aagagatcat catcagcatc catcatggct ggtctttttg gttccgccgc
     5521 cgcatccgcg agcaacaccc tcggcgatct taagcaggat gtcgagctcg gccaaccccc
     5581 cgaggacagc atcagcgacc ttgccttcaa ccccaacccc gccgaccaga aggacttcct
     5641 cgccgtagcc agctgggaca agaagacacg catttacgag atcttgagta atggtcaagg
     5701 tcagggacag gccatgattg agcatgatgc ccccgttttc tcttgcgatt tcttcaaggt
     5761 atgtttgcgt cgattggcct ggcaggtagc ccgacaaaac actgacatgc acttctccca
     5821 acaggacggc acaaaggtca tttccgccgg cgccgacaag gccgccaagg ttctcgattt
     5881 ggccaccggt cagtcgatgc aggtcgctgc tcacgatatg cccatcaagt gcgttcgcta
     5941 cttcgaggcc aatggcacgc ccatggcggt gacaggtggc tgggacaagc agatcaagta
     6001 ctgggatttc cgctccgcga acccggccgc cacggtgcag gcccaggagc gcgtttatac
     6061 catggatgtg cgcgacaact tgctcgtggt cggaaccgct gatcgctata tcaacgtgat
     6121 caacctgaag gaccccggca agttctacaa gacaatgcag agccctctta aatggcagac
     6181 tcgtgtggtc agctgcttca acgattctca gggctttgct atcggctcga tcgagggtag
     6241 atgcgccatt caatatgtgg aggacaagga ttctgcgtac gccttcacct cctccccctt
     6301 tactatccca tctgccgaat acgctgacta actgacttgt tttccagctc caacttctct
     6361 ttcaaatgcc accgcgaccc cgcccaaggc aacaccacgg ccgtccacgc tgtcaacgac
     6421 atctccttcc acccccaaca cggcaccttc agcacagccg gtagcgacgg caccttccac
     6481 ttctgggaca aggacgccaa gcaccgtctc aagggttacc ccaacgtcgg cggcagcatc
     6541 acctcgacaa cgttcaacaa gaccggcagc atctttgcct acgccattag ttatgactgg
     6601 agcaaggggt accaaggcaa ctcgcccacg tacccgacca aggtcatgct ccacccagta
     6661 cagcaggacg agtgcaagcc ccgtccgagc gttaagaaga gggctggtat tggtttggct
     6721 attgctggca tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg
     6781 gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaga
     6841 ccgtacgagg gagtgcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt
     6901 gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacctttat caagtacccg
     6961 gccgacatcc ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt
     7021 acgagatacg aagatggtgg agtcgtcacc gtcacgcagg acaccagcct tgaggatggc
     7081 gagctcgtct acaacgtcaa ggtcagaggg gtaaactttc cctccaatgg tcccgtgatg
     7141 cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt
     7201 ctgagaggat acactgacat cgcactgaaa gttgatggtg gtggccatct gcactgcaac
     7261 ttcgtgacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtgtccat
     7321 gccgttgatc accgcctgga aaggatcgag gagagtgaca atgaaaccta cgtagtgcaa
     7381 agagaagtgg cagttgccaa atacagcaac cttggtggtg gcatggacga gctgtacaag
     7441 tgactagagg atccgacact atagaatacc tagacagttt gcaggtcatg tgaatgttat
     7501 cgccaacaca ttgaatgcta aacagattac ctccaacgcc tagatgaaac caaattaccc
     7561 ttgccttctt tttttttacg ccgttaatgc agttgtgata gagaccatgc aacgtcttct
     7621 aaacggagac acaaacacat tcgagtcgat cagatcgaaa tgacttgttc ctgtacctcg
     7681 tcaccatcca gccaccgtct gaaatagacg cagttgctgg ggctgacagg tccgccttcc
     7741 tcgcacatat catagacagg gcaccgccca caaggcgcct ccataaggcc acttgcactc
     7801 ggctcaactc ccataaatga ggtaacaatg cctcctttgg ttggatcatg gtcgaggcgt
     7861 cgttgattgc acgtcctcgg atcctgtttg gggatgcgcg tcacacggta ccccttgcgc
     7921 ccattgacct tgacaggctc tactaagcca tcgtagtata gcacatccac caactgctgg
     7981 atatccgcct cgctcaacgt cgtattattc gtgatgccgg cgtggtggat gaactttgca
     8041 atgtccgaca cagtcgggta cgacttgtaa ccggccggga gaggcagcaa ttgcggcttg
     8101 ccgggcctgg tcttcttggc cggtgccggt gccgccgtgt cgtcgttgga aatctcgccc
     8161 gcgctgcgct tcgtcccctt ggaaatatcc tccgagtggg gaatggtccc ctgaaccacg
     8221 cccttcttgg gattcttttc acgctgctgt gctcctcctc cttgtgctcc tactcctggt
     8281 actccagcac cgccgccgtg ggaagtatat gtcgatttct gcttgataaa gtcaaacaca
     8341 atttcctgta gctcgttgat gaaggccgtg tcgagctcgc cctcagtaaa ccaggggcca
     8401 cctgtggcgc gatcggaggg acgtaggttc gccttgatgt acatcttctt gttggggtgc
     8461 tcgacgttct tcatgctggc gatgtagccc ttttgctcga gatacttgat gcaatgcttc
     8521 ataagcgagt cggccatgtt gagcttgttc ttgatggtcc ggttccagat gccgtcggcc
     8581 ccggcttcgt cgaccatggc gtataccatc attatttcgg agttggggag ggaggtgtac
     8641 cttttgggag gaaggggagg ttagttggtg tgctcggttg gctgtttcct gtgtgaaatt
     8701 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8761 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8821 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8881 tgtgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9481 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9721 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9781 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10141 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10441 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagg
//
