LOCUS       sequence-379325-       10719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mScarlet
FEATURES             Location/Qualifiers
     source          1..10719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(533..1324,1325..1393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1156..1175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1394..1498)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1525..2867
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2488..2535)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2908..2926
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2964..2986)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3086..3105
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3129..3344
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3345..4148
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(4279..4734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4416..4437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4628..4647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             7661..7672
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8161..8856
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(8294..8312)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8562..8581
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(10104..10126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10115..10131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10139..10145,10146..10163,10164..10169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10184..10205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10322..10339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10493..10719,1..362))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10573..10592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
       61 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      181 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      301 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1501 catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     1561 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     1621 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     1681 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     1741 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     1801 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     1861 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     1921 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     1981 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     2041 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     2101 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     2161 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     2221 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     2281 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     2341 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     2401 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     2461 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
     2521 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
     2581 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
     2641 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
     2701 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
     2761 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
     2821 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc
     2881 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     2941 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3001 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3061 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     3121 accacctttt caattcatca tttttttttt attctttttt ttgatttcgg tttccttgaa
     3181 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
     3241 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
     3301 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
     3361 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
     3421 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
     3481 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
     3541 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
     3601 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
     3661 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
     3721 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
     3781 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
     3841 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
     3901 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
     3961 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
     4021 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
     4081 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
     4141 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
     4201 caatttaatt atatcagtta ttaccctatg cggtgtgaaa taccgcacag atgcgtaagg
     4261 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     4321 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     4381 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     4441 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     4501 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4561 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4621 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4681 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     4741 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     4801 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     4861 ccagtcacga cgctggagaa gctcattcca cccgaaatgc ccgtgaactt gcccttgttt
     4921 gcgcgggcct tcttgcgctc tgctcggata cgctcgacat cgctgaggag ctcggcgagt
     4981 tccttggcgc ggttgcggac gttgataccc tggtctttgc cgttctggtc gataaaatgg
     5041 aactggcgca gcatcttgag gagggtaaga tgcgaccgag catcgtccac cactcgttcg
     5101 gaaccgtgct taatcaggta ctctagtagt tgaagggcct tgtagatctg gcgccattct
     5161 tcggcggact tttctgtgaa acggcggtaa atcatgggca tgatttcgtt gagtgtttgg
     5221 ctaagaacaa ggagggaaac gcaagttagc cgcgttcgta tacaagcgag cagttctcga
     5281 gaacgtacta gttgaaggtg ccatcggcga tctcttgcat caaagtcgac gatgcacccc
     5341 aaggttcatt gttggtggcc tcgcgcacct gggttcgctg tcagctcccg ttccatggtc
     5401 tcttgtctgg gatcaagtat atggcgaagg gcgcaagaga atggtgccag tgtggcgcgt
     5461 ggatacgggc ataccttgga ctccatctcg gtatagttca tgacagctgg ttggagccat
     5521 tgtcagcacc ctgaacacga aatcatgagg gagtcttggt tctggactag gtggtcacct
     5581 accattttga acctttcgta cgccagcctt gatgtcgtag agtgtcaggt tggacacggt
     5641 atccttgagg ctattgaaat ccattgcgta aacgtgtcta tcgccggagt tgctgggaat
     5701 tgttctgatg ggctaaggta attcttctct agatccaatc tgagcctcgc ggtgatgatt
     5761 gccagacggg cgggatgctg tggcgacttg gtggtttttg ttcgggctga tcgctttcag
     5821 ggcgacgttt cacggtcttg tcgggatcag atggacattg aggtgcggaa ttatacgatt
     5881 taggtgactg caggccatac agatctccct agacgccggg ccaatccgtt ccttgcacct
     5941 tctctcacac atgcaggttt tggaatgtgg cttcatcgtg tccagaactg ggcgcattgg
     6001 ggccaggctc agtgtcagtg tagggatcga tgtacatatg gcagttgaca gcgagagagc
     6061 caggtgtcac aggcagtccg tcagtggccg cggtggtggc cacgcacttt ctgcaggagc
     6121 cgtctggggt gcgtgcagct ggcggccgct tccgggcata cggcttgtgt aacgagggtg
     6181 ttgaccctcg gcttggcgct tgaacttggc gctgcggtcc ccccacagtt gaccacaatc
     6241 cccggccaat cgaacgacgg caacaagggc gcactagcca gtgaacgcct ggggatagtg
     6301 ggtggtcaaa gagaattggg caaacgagac gacaatggag ggtgaaccgg gaataataaa
     6361 cagctggtga gaccacccat caaacgaatc accccaccat gggatgccct cagatgccct
     6421 ccatgccacc ataaggaatg atcttacggc ttgccgaagt taacaggcct gcttttgcat
     6481 atgaacgaga gcctggtgtt gcattccaca ttggctatct acagtaccta cccaacctac
     6541 acaccctgat gcgaaaggga cagaagggga gaaggataca tacatatacc tgactcgtgc
     6601 ccatcaacaa cagagaaagg gtgatcgctg aacacatcag agcatctctt cccccctggc
     6661 gactaaaagt gctcccaatc tcgtcttaca tctgatacgt tgttcaaact ccgcaacccg
     6721 atctgcacac tgccctgcct tggcccttct tggagtctcc gcttccccac gccatctcac
     6781 gctcgaggac tccgctgcta ggtatgagct aggcctgccg tcgacaggat ccaccagacg
     6841 tcaacaataa cccatcttgg aaatacataa gaagacgtca cttccctccc ttttcgaaag
     6901 gaagagatca tcatcagcat ccatcatggc tggtcttttt ggttccgccg ccgcatccgc
     6961 gagcaacacc ctcggcgatc ttaagcagga tgtcgagctc ggccaacccc ccgaggacag
     7021 catcagcgac cttgccttca accccaaccc cgccgaccag aaggacttcc tcgccgtagc
     7081 cagctgggac aagaagacac gcatttacga gatcttgagt aatggtcaag gtcagggaca
     7141 ggccatgatt gagcatgatg cccccgtttt ctcttgcgat ttcttcaagg tatgtttgcg
     7201 tcgattggcc tggcaggtag cccgacaaaa cactgacatg cacttctccc aacaggacgg
     7261 cacaaaggtc atttccgccg gcgccgacaa ggccgccaag gttctcgatt tggccaccgg
     7321 tcagtcgatg caggtcgctg ctcacgatat gcccatcaag tgcgttcgct acttcgaggc
     7381 caatggcacg cccatggcgg tgacaggtgg ctgggacaag cagatcaagt actgggattt
     7441 ccgctccgcg aacccggccg ccacggtgca ggcccaggag cgcgtttata ccatggatgt
     7501 gcgcgacaac ttgctcgtgg tcggaaccgc tgatcgctat atcaacgtga tcaacctgaa
     7561 ggaccccggc aagttctaca agacaatgca gagccctctt aaatggcaga ctcgtgtggt
     7621 cagctgcttc aacgattctc agggctttgc tatcggctcg atcgagggta gatgcgccat
     7681 tcaatatgtg gaggacaagg attctgcgta cgccttcacc tcctccccct ttactatccc
     7741 atctgccgaa tacgctgact aactgacttg ttttccagct ccaacttctc tttcaaatgc
     7801 caccgcgacc ccgcccaagg caacaccacg gccgtccacg ctgtcaacga catctccttc
     7861 cacccccaac acggcacctt cagcacagcc ggtagcgacg gcaccttcca cttctgggac
     7921 aaggacgcca agcaccgtct caagggttac cccaacgtcg gcggcagcat cacctcgaca
     7981 acgttcaaca agaccggcag catctttgcc tacgccatta gttatgactg gagcaagggg
     8041 taccaaggca actcgcccac gtacccgacc aaggtcatgc tccacccagt acagcaggac
     8101 gagtgcaagc cccgtccgag cgttaagaag agggctggta ttggtttggc tattgctggc
     8161 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
     8221 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     8281 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
     8341 atcctgtccc ctcagttcat gtacggctcc agggccttca ccaagcaccc cgccgacatc
     8401 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     8461 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
     8521 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
     8581 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
     8641 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
     8701 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
     8761 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
     8821 cgccactcca ccggcggcat ggacgagctg tacaagtgac tagaggatcc gacactatag
     8881 aatacctaga cagtttgcag gtcatgtgaa tgttatcgcc aacacattga atgctaaaca
     8941 gattacctcc aacgcctaga tgaaaccaaa ttacccttgc cttctttttt tttacgccgt
     9001 taatgcagtt gtgatagaga ccatgcaacg tcttctaaac ggagacacaa acacattcga
     9061 gtcgatcaga tcgaaatgac ttgttcctgt acctcgtcac catccagcca ccgtctgaaa
     9121 tagacgcagt tgctggggct gacaggtccg ccttcctcgc acatatcata gacagggcac
     9181 cgcccacaag gcgcctccat aaggccactt gcactcggct caactcccat aaatgaggta
     9241 acaatgcctc ctttggttgg atcatggtcg aggcgtcgtt gattgcacgt cctcggatcc
     9301 tgtttgggga tgcgcgtcac acggtacccc ttgcgcccat tgaccttgac aggctctact
     9361 aagccatcgt agtatagcac atccaccaac tgctggatat ccgcctcgct caacgtcgta
     9421 ttattcgtga tgccggcgtg gtggatgaac tttgcaatgt ccgacacagt cgggtacgac
     9481 ttgtaaccgg ccgggagagg cagcaattgc ggcttgccgg gcctggtctt cttggccggt
     9541 gccggtgccg ccgtgtcgtc gttggaaatc tcgcccgcgc tgcgcttcgt ccccttggaa
     9601 atatcctccg agtggggaat ggtcccctga accacgccct tcttgggatt cttttcacgc
     9661 tgctgtgctc ctcctccttg tgctcctact cctggtactc cagcaccgcc gccgtgggaa
     9721 gtatatgtcg atttctgctt gataaagtca aacacaattt cctgtagctc gttgatgaag
     9781 gccgtgtcga gctcgccctc agtaaaccag gggccacctg tggcgcgatc ggagggacgt
     9841 aggttcgcct tgatgtacat cttcttgttg gggtgctcga cgttcttcat gctggcgatg
     9901 tagccctttt gctcgagata cttgatgcaa tgcttcataa gcgagtcggc catgttgagc
     9961 ttgttcttga tggtccggtt ccagatgccg tcggccccgg cttcgtcgac catggcgtat
    10021 accatcatta tttcggagtt ggggagggag gtgtaccttt tgggaggaag gggaggttag
    10081 ttggtgtgct cggttggctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10261 attaatgaat cggccaacgc gcggggagag gcggtttgtg tattgggcgc tcttccgctt
    10321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10681 tttctcatag ctcacgctgt aggtatctca gttcggtgt
//
