LOCUS       sequence-379326-       10734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mTagBFP2
FEATURES             Location/Qualifiers
     source          1..10734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1947..3289
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2910..2957)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3330..3348
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3386..3408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3508..3527
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3551..3766
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3767..4570
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(4701..5156)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4838..4859)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5050..5069
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             8083..8094
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10541..10563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10552..10568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10576..10582,10583..10600,10601..10606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10621..10642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa
     1981 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     2041 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa
     2101 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     2161 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt
     2221 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt
     2281 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg
     2341 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact
     2401 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg
     2461 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat
     2521 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta
     2581 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
     2641 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
     2701 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
     2761 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
     2821 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
     2881 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac
     2941 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     3001 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     3061 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     3121 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     3181 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
     3241 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa
     3301 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     3361 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3421 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3481 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     3541 ataccacctt ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg
     3601 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
     3661 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
     3721 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac
     3781 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
     3841 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
     3901 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
     3961 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
     4021 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
     4081 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
     4141 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
     4201 aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac
     4261 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
     4321 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
     4381 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc
     4441 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
     4501 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
     4561 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
     4621 ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac agatgcgtaa
     4681 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4741 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4801 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4861 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4921 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4981 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5041 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     5101 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     5161 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5221 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5281 tcccagtcac gacgctggag aagctcattc cacccgaaat gcccgtgaac ttgcccttgt
     5341 ttgcgcgggc cttcttgcgc tctgctcgga tacgctcgac atcgctgagg agctcggcga
     5401 gttccttggc gcggttgcgg acgttgatac cctggtcttt gccgttctgg tcgataaaat
     5461 ggaactggcg cagcatcttg aggagggtaa gatgcgaccg agcatcgtcc accactcgtt
     5521 cggaaccgtg cttaatcagg tactctagta gttgaagggc cttgtagatc tggcgccatt
     5581 cttcggcgga cttttctgtg aaacggcggt aaatcatggg catgatttcg ttgagtgttt
     5641 ggctaagaac aaggagggaa acgcaagtta gccgcgttcg tatacaagcg agcagttctc
     5701 gagaacgtac tagttgaagg tgccatcggc gatctcttgc atcaaagtcg acgatgcacc
     5761 ccaaggttca ttgttggtgg cctcgcgcac ctgggttcgc tgtcagctcc cgttccatgg
     5821 tctcttgtct gggatcaagt atatggcgaa gggcgcaaga gaatggtgcc agtgtggcgc
     5881 gtggatacgg gcataccttg gactccatct cggtatagtt catgacagct ggttggagcc
     5941 attgtcagca ccctgaacac gaaatcatga gggagtcttg gttctggact aggtggtcac
     6001 ctaccatttt gaacctttcg tacgccagcc ttgatgtcgt agagtgtcag gttggacacg
     6061 gtatccttga ggctattgaa atccattgcg taaacgtgtc tatcgccgga gttgctggga
     6121 attgttctga tgggctaagg taattcttct ctagatccaa tctgagcctc gcggtgatga
     6181 ttgccagacg ggcgggatgc tgtggcgact tggtggtttt tgttcgggct gatcgctttc
     6241 agggcgacgt ttcacggtct tgtcgggatc agatggacat tgaggtgcgg aattatacga
     6301 tttaggtgac tgcaggccat acagatctcc ctagacgccg ggccaatccg ttccttgcac
     6361 cttctctcac acatgcaggt tttggaatgt ggcttcatcg tgtccagaac tgggcgcatt
     6421 ggggccaggc tcagtgtcag tgtagggatc gatgtacata tggcagttga cagcgagaga
     6481 gccaggtgtc acaggcagtc cgtcagtggc cgcggtggtg gccacgcact ttctgcagga
     6541 gccgtctggg gtgcgtgcag ctggcggccg cttccgggca tacggcttgt gtaacgaggg
     6601 tgttgaccct cggcttggcg cttgaacttg gcgctgcggt ccccccacag ttgaccacaa
     6661 tccccggcca atcgaacgac ggcaacaagg gcgcactagc cagtgaacgc ctggggatag
     6721 tgggtggtca aagagaattg ggcaaacgag acgacaatgg agggtgaacc gggaataata
     6781 aacagctggt gagaccaccc atcaaacgaa tcaccccacc atgggatgcc ctcagatgcc
     6841 ctccatgcca ccataaggaa tgatcttacg gcttgccgaa gttaacaggc ctgcttttgc
     6901 atatgaacga gagcctggtg ttgcattcca cattggctat ctacagtacc tacccaacct
     6961 acacaccctg atgcgaaagg gacagaaggg gagaaggata catacatata cctgactcgt
     7021 gcccatcaac aacagagaaa gggtgatcgc tgaacacatc agagcatctc ttcccccctg
     7081 gcgactaaaa gtgctcccaa tctcgtctta catctgatac gttgttcaaa ctccgcaacc
     7141 cgatctgcac actgccctgc cttggccctt cttggagtct ccgcttcccc acgccatctc
     7201 acgctcgagg actccgctgc taggtatgag ctaggcctgc cgtcgacagg atccaccaga
     7261 cgtcaacaat aacccatctt ggaaatacat aagaagacgt cacttccctc ccttttcgaa
     7321 aggaagagat catcatcagc atccatcatg gctggtcttt ttggttccgc cgccgcatcc
     7381 gcgagcaaca ccctcggcga tcttaagcag gatgtcgagc tcggccaacc ccccgaggac
     7441 agcatcagcg accttgcctt caaccccaac cccgccgacc agaaggactt cctcgccgta
     7501 gccagctggg acaagaagac acgcatttac gagatcttga gtaatggtca aggtcaggga
     7561 caggccatga ttgagcatga tgcccccgtt ttctcttgcg atttcttcaa ggtatgtttg
     7621 cgtcgattgg cctggcaggt agcccgacaa aacactgaca tgcacttctc ccaacaggac
     7681 ggcacaaagg tcatttccgc cggcgccgac aaggccgcca aggttctcga tttggccacc
     7741 ggtcagtcga tgcaggtcgc tgctcacgat atgcccatca agtgcgttcg ctacttcgag
     7801 gccaatggca cgcccatggc ggtgacaggt ggctgggaca agcagatcaa gtactgggat
     7861 ttccgctccg cgaacccggc cgccacggtg caggcccagg agcgcgttta taccatggat
     7921 gtgcgcgaca acttgctcgt ggtcggaacc gctgatcgct atatcaacgt gatcaacctg
     7981 aaggaccccg gcaagttcta caagacaatg cagagccctc ttaaatggca gactcgtgtg
     8041 gtcagctgct tcaacgattc tcagggcttt gctatcggct cgatcgaggg tagatgcgcc
     8101 attcaatatg tggaggacaa ggattctgcg tacgccttca cctcctcccc ctttactatc
     8161 ccatctgccg aatacgctga ctaactgact tgttttccag ctccaacttc tctttcaaat
     8221 gccaccgcga ccccgcccaa ggcaacacca cggccgtcca cgctgtcaac gacatctcct
     8281 tccaccccca acacggcacc ttcagcacag ccggtagcga cggcaccttc cacttctggg
     8341 acaaggacgc caagcaccgt ctcaagggtt accccaacgt cggcggcagc atcacctcga
     8401 caacgttcaa caagaccggc agcatctttg cctacgccat tagttatgac tggagcaagg
     8461 ggtaccaagg caactcgccc acgtacccga ccaaggtcat gctccaccca gtacagcagg
     8521 acgagtgcaa gccccgtccg agcgttaaga agagggctgg tattggtttg gctattgctg
     8581 gcatggtctc caagggcgag gagctcatca aggagaacat gcacatgaag ctctacatgg
     8641 agggcaccgt cgacaaccac cacttcaagt gcacctccga gggcgagggc aagccctacg
     8701 agggcaccca gaccatgcgc atcaaggtcg tcgagggcgg ccccctcccc ttcgccttcg
     8761 acatcctcgc cacctccttc ctctacggct ccaagacctt catcaaccac acccagggca
     8821 tccccgactt cttcaagcag tccttccccg agggcttcac ctgggagcgc gtcaccacct
     8881 acgaggacgg cggcgtcctc accgccaccc aggacacctc cctccaggac ggctgcctca
     8941 tctacaacgt caagatccgc ggcgtcaact tcacctccaa cggccccgtc atgcagaaga
     9001 agaccctcgg ctgggaggcc ttcaccgaga ccctctaccc cgccgacggc ggcctcgagg
     9061 gccgcaacga catggccctc aagctcgtcg gcggctccca cctcatcgcc aacgccaaga
     9121 ccacctaccg ctccaagaag cccgccaaga acctcaagat gcccggcgtc tactacgtcg
     9181 actaccgcct cgagcgcatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     9241 tcgccgtcgc ccgctactgc gacctcccct ccaagctcgg ccacaagctc aactgactag
     9301 aggatccgac actatagaat acctagacag tttgcaggtc atgtgaatgt tatcgccaac
     9361 acattgaatg ctaaacagat tacctccaac gcctagatga aaccaaatta cccttgcctt
     9421 cttttttttt acgccgttaa tgcagttgtg atagagacca tgcaacgtct tctaaacgga
     9481 gacacaaaca cattcgagtc gatcagatcg aaatgacttg ttcctgtacc tcgtcaccat
     9541 ccagccaccg tctgaaatag acgcagttgc tggggctgac aggtccgcct tcctcgcaca
     9601 tatcatagac agggcaccgc ccacaaggcg cctccataag gccacttgca ctcggctcaa
     9661 ctcccataaa tgaggtaaca atgcctcctt tggttggatc atggtcgagg cgtcgttgat
     9721 tgcacgtcct cggatcctgt ttggggatgc gcgtcacacg gtaccccttg cgcccattga
     9781 ccttgacagg ctctactaag ccatcgtagt atagcacatc caccaactgc tggatatccg
     9841 cctcgctcaa cgtcgtatta ttcgtgatgc cggcgtggtg gatgaacttt gcaatgtccg
     9901 acacagtcgg gtacgacttg taaccggccg ggagaggcag caattgcggc ttgccgggcc
     9961 tggtcttctt ggccggtgcc ggtgccgccg tgtcgtcgtt ggaaatctcg cccgcgctgc
    10021 gcttcgtccc cttggaaata tcctccgagt ggggaatggt cccctgaacc acgcccttct
    10081 tgggattctt ttcacgctgc tgtgctcctc ctccttgtgc tcctactcct ggtactccag
    10141 caccgccgcc gtgggaagta tatgtcgatt tctgcttgat aaagtcaaac acaatttcct
    10201 gtagctcgtt gatgaaggcc gtgtcgagct cgccctcagt aaaccagggg ccacctgtgg
    10261 cgcgatcgga gggacgtagg ttcgccttga tgtacatctt cttgttgggg tgctcgacgt
    10321 tcttcatgct ggcgatgtag cccttttgct cgagatactt gatgcaatgc ttcataagcg
    10381 agtcggccat gttgagcttg ttcttgatgg tccggttcca gatgccgtcg gccccggctt
    10441 cgtcgaccat ggcgtatacc atcattattt cggagttggg gagggaggtg taccttttgg
    10501 gaggaagggg aggttagttg gtgtgctcgg ttggctgttt cctgtgtgaa attgttatcc
    10561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gttt
//
