LOCUS       sequence-379327-       11082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_vsfGFP9
FEATURES             Location/Qualifiers
     source          1..11082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(527..1318,1319..1387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1150..1169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1388..1492)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1519..2861
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2482..2529)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2902..2920
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2958..2980)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3080..3099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3123..3338
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3339..4142
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(4273..4728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4410..4431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4622..4641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             7655..7666
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(8155..8157,8158..8868)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8875..9213
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVS"
     primer_bind     complement(10461..10483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10472..10488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10496..10502,10503..10520,10521..10526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10541..10562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10679..10696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10850..11082,1..356))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10930..10949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
       61 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      121 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      181 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      241 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      301 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      361 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      421 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      481 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      541 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      601 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      661 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      721 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      781 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      841 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      901 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      961 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1021 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1081 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1141 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1201 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1261 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1321 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1381 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1441 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1501 cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac
     1561 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     1621 aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat
     1681 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     1741 aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca
     1801 aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc
     1861 tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt
     1921 agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc
     1981 cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg
     2041 attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga
     2101 tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac
     2161 gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact
     2221 acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt
     2281 ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag
     2341 agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt
     2401 tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct
     2461 tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat
     2521 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
     2581 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
     2641 gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg
     2701 taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt
     2761 atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta
     2821 gtctcatcct tcaatgctat catttccttt gatattggat catactaaga aaccattatt
     2881 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     2941 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3001 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3061 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccataccacc
     3121 ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct tgaaattttt
     3181 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     3241 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     3301 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     3361 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     3421 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     3481 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     3541 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     3601 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     3661 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     3721 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     3781 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     3841 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     3901 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     3961 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     4021 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     4081 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     4141 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     4201 aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4261 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4321 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4381 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4441 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4501 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4561 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4621 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4681 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     4741 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     4801 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     4861 acgacgctgg agaagctcat tccacccgaa atgcccgtga acttgccctt gtttgcgcgg
     4921 gccttcttgc gctctgctcg gatacgctcg acatcgctga ggagctcggc gagttccttg
     4981 gcgcggttgc ggacgttgat accctggtct ttgccgttct ggtcgataaa atggaactgg
     5041 cgcagcatct tgaggagggt aagatgcgac cgagcatcgt ccaccactcg ttcggaaccg
     5101 tgcttaatca ggtactctag tagttgaagg gccttgtaga tctggcgcca ttcttcggcg
     5161 gacttttctg tgaaacggcg gtaaatcatg ggcatgattt cgttgagtgt ttggctaaga
     5221 acaaggaggg aaacgcaagt tagccgcgtt cgtatacaag cgagcagttc tcgagaacgt
     5281 actagttgaa ggtgccatcg gcgatctctt gcatcaaagt cgacgatgca ccccaaggtt
     5341 cattgttggt ggcctcgcgc acctgggttc gctgtcagct cccgttccat ggtctcttgt
     5401 ctgggatcaa gtatatggcg aagggcgcaa gagaatggtg ccagtgtggc gcgtggatac
     5461 gggcatacct tggactccat ctcggtatag ttcatgacag ctggttggag ccattgtcag
     5521 caccctgaac acgaaatcat gagggagtct tggttctgga ctaggtggtc acctaccatt
     5581 ttgaaccttt cgtacgccag ccttgatgtc gtagagtgtc aggttggaca cggtatcctt
     5641 gaggctattg aaatccattg cgtaaacgtg tctatcgccg gagttgctgg gaattgttct
     5701 gatgggctaa ggtaattctt ctctagatcc aatctgagcc tcgcggtgat gattgccaga
     5761 cgggcgggat gctgtggcga cttggtggtt tttgttcggg ctgatcgctt tcagggcgac
     5821 gtttcacggt cttgtcggga tcagatggac attgaggtgc ggaattatac gatttaggtg
     5881 actgcaggcc atacagatct ccctagacgc cgggccaatc cgttccttgc accttctctc
     5941 acacatgcag gttttggaat gtggcttcat cgtgtccaga actgggcgca ttggggccag
     6001 gctcagtgtc agtgtaggga tcgatgtaca tatggcagtt gacagcgaga gagccaggtg
     6061 tcacaggcag tccgtcagtg gccgcggtgg tggccacgca ctttctgcag gagccgtctg
     6121 gggtgcgtgc agctggcggc cgcttccggg catacggctt gtgtaacgag ggtgttgacc
     6181 ctcggcttgg cgcttgaact tggcgctgcg gtccccccac agttgaccac aatccccggc
     6241 caatcgaacg acggcaacaa gggcgcacta gccagtgaac gcctggggat agtgggtggt
     6301 caaagagaat tgggcaaacg agacgacaat ggagggtgaa ccgggaataa taaacagctg
     6361 gtgagaccac ccatcaaacg aatcacccca ccatgggatg ccctcagatg ccctccatgc
     6421 caccataagg aatgatctta cggcttgccg aagttaacag gcctgctttt gcatatgaac
     6481 gagagcctgg tgttgcattc cacattggct atctacagta cctacccaac ctacacaccc
     6541 tgatgcgaaa gggacagaag gggagaagga tacatacata tacctgactc gtgcccatca
     6601 acaacagaga aagggtgatc gctgaacaca tcagagcatc tcttcccccc tggcgactaa
     6661 aagtgctccc aatctcgtct tacatctgat acgttgttca aactccgcaa cccgatctgc
     6721 acactgccct gccttggccc ttcttggagt ctccgcttcc ccacgccatc tcacgctcga
     6781 ggactccgct gctaggtatg agctaggcct gccgtcgaca ggatccacca gacgtcaaca
     6841 ataacccatc ttggaaatac ataagaagac gtcacttccc tcccttttcg aaaggaagag
     6901 atcatcatca gcatccatca tggctggtct ttttggttcc gccgccgcat ccgcgagcaa
     6961 caccctcggc gatcttaagc aggatgtcga gctcggccaa ccccccgagg acagcatcag
     7021 cgaccttgcc ttcaacccca accccgccga ccagaaggac ttcctcgccg tagccagctg
     7081 ggacaagaag acacgcattt acgagatctt gagtaatggt caaggtcagg gacaggccat
     7141 gattgagcat gatgcccccg ttttctcttg cgatttcttc aaggtatgtt tgcgtcgatt
     7201 ggcctggcag gtagcccgac aaaacactga catgcacttc tcccaacagg acggcacaaa
     7261 ggtcatttcc gccggcgccg acaaggccgc caaggttctc gatttggcca ccggtcagtc
     7321 gatgcaggtc gctgctcacg atatgcccat caagtgcgtt cgctacttcg aggccaatgg
     7381 cacgcccatg gcggtgacag gtggctggga caagcagatc aagtactggg atttccgctc
     7441 cgcgaacccg gccgccacgg tgcaggccca ggagcgcgtt tataccatgg atgtgcgcga
     7501 caacttgctc gtggtcggaa ccgctgatcg ctatatcaac gtgatcaacc tgaaggaccc
     7561 cggcaagttc tacaagacaa tgcagagccc tcttaaatgg cagactcgtg tggtcagctg
     7621 cttcaacgat tctcagggct ttgctatcgg ctcgatcgag ggtagatgcg ccattcaata
     7681 tgtggaggac aaggattctg cgtacgcctt cacctcctcc ccctttacta tcccatctgc
     7741 cgaatacgct gactaactga cttgttttcc agctccaact tctctttcaa atgccaccgc
     7801 gaccccgccc aaggcaacac cacggccgtc cacgctgtca acgacatctc cttccacccc
     7861 caacacggca ccttcagcac agccggtagc gacggcacct tccacttctg ggacaaggac
     7921 gccaagcacc gtctcaaggg ttaccccaac gtcggcggca gcatcacctc gacaacgttc
     7981 aacaagaccg gcagcatctt tgcctacgcc attagttatg actggagcaa ggggtaccaa
     8041 ggcaactcgc ccacgtaccc gaccaaggtc atgctccacc cagtacagca ggacgagtgc
     8101 aagccccgtc cgagcgttaa gaagagggct ggtattggtt tggctattgc tggcatgagc
     8161 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaactcga tggtgatgtt
     8221 aatggccaca aattttccgt ccgcggagag ggtgaaggtg atgctacaaa cggaaaactc
     8281 acccttaaat ttatttgcac tactggaaaa ctccctgttc cgtggccaac acttgtcact
     8341 actctgacct atggtgttca atgcttttcc cgctatccgg atcacatgaa acgccatgac
     8401 tttttcaagt ccgccatgcc cgaaggttat gtccaggaac gcactatctc cttcaaagat
     8461 gacggcacct acaagacgcg cgctgaagtc aagtttgaag gtgataccct tgttaatcgc
     8521 atcgagctca agggcattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     8581 tacaacttta actcccacaa tgtctacatc acggccgaca aacaaaagaa tggaatcaaa
     8641 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactcgc cgaccattat
     8701 caacaaaata ctccaattgg cgatggccct gtccttctcc cagacaacca ttacctgtcg
     8761 acacaatccg tcctttcgaa agatcccaac gaaaagcgcg accacatggt ccttcttgag
     8821 tttgtcactg ctgctggcat tacacatggc atggatgagc tctacaaagc ccaggtgcag
     8881 ttggtcgaat ccggcggtgc cctggttcaa ccgggtggta gcctgcgcct gtcctgtgcg
     8941 gcgtccggct tcccagttaa tcgctatagc atgcgctggt accgccaagc gcctggcaag
     9001 gagcgcgagt gggtcgcggg tatgagctcg gcgggtgatc gcagcagcta cgaagattcc
     9061 gttaaaggcc gctttaccat cagccgcgac gatgcgcgca ataccgtcta cctgcaaatg
     9121 aacagcctga agccggagga caccgccgtc tattactgca atgttaatgt gggttttgaa
     9181 tactggggcc agggtacgca agttaccgtt tcctaactag aggatccgac actatagaat
     9241 acctagacag tttgcaggtc atgtgaatgt tatcgccaac acattgaatg ctaaacagat
     9301 tacctccaac gcctagatga aaccaaatta cccttgcctt cttttttttt acgccgttaa
     9361 tgcagttgtg atagagacca tgcaacgtct tctaaacgga gacacaaaca cattcgagtc
     9421 gatcagatcg aaatgacttg ttcctgtacc tcgtcaccat ccagccaccg tctgaaatag
     9481 acgcagttgc tggggctgac aggtccgcct tcctcgcaca tatcatagac agggcaccgc
     9541 ccacaaggcg cctccataag gccacttgca ctcggctcaa ctcccataaa tgaggtaaca
     9601 atgcctcctt tggttggatc atggtcgagg cgtcgttgat tgcacgtcct cggatcctgt
     9661 ttggggatgc gcgtcacacg gtaccccttg cgcccattga ccttgacagg ctctactaag
     9721 ccatcgtagt atagcacatc caccaactgc tggatatccg cctcgctcaa cgtcgtatta
     9781 ttcgtgatgc cggcgtggtg gatgaacttt gcaatgtccg acacagtcgg gtacgacttg
     9841 taaccggccg ggagaggcag caattgcggc ttgccgggcc tggtcttctt ggccggtgcc
     9901 ggtgccgccg tgtcgtcgtt ggaaatctcg cccgcgctgc gcttcgtccc cttggaaata
     9961 tcctccgagt ggggaatggt cccctgaacc acgcccttct tgggattctt ttcacgctgc
    10021 tgtgctcctc ctccttgtgc tcctactcct ggtactccag caccgccgcc gtgggaagta
    10081 tatgtcgatt tctgcttgat aaagtcaaac acaatttcct gtagctcgtt gatgaaggcc
    10141 gtgtcgagct cgccctcagt aaaccagggg ccacctgtgg cgcgatcgga gggacgtagg
    10201 ttcgccttga tgtacatctt cttgttgggg tgctcgacgt tcttcatgct ggcgatgtag
    10261 cccttttgct cgagatactt gatgcaatgc ttcataagcg agtcggccat gttgagcttg
    10321 ttcttgatgg tccggttcca gatgccgtcg gccccggctt cgtcgaccat ggcgtatacc
    10381 atcattattt cggagttggg gagggaggtg taccttttgg gaggaagggg aggttagttg
    10441 gtgtgctcgg ttggctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10501 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10621 aatgaatcgg ccaacgcgcg gggagaggcg gtttgtgtat tgggcgctct tccgcttcct
    10681 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cg
//
