LOCUS       sequence-379329-       10755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_mNeptune2.5
FEATURES             Location/Qualifiers
     source          1..10755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             complement(3089..3100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      6027..6482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6114..6133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6324..6345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6613..7416)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7417..7632)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7656..7675)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7775..7797
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7835..7853)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      7894..9236
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8226..8273
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9263..9367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9368..9436,9437..10228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9586..9605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10399..10755,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacacaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcca accgagcaca ccaactaacc tccccttcct
      661 cccaaaaggt acacctccct ccccaactcc gaaataatga tggtatacgc catggtcgac
      721 gaagccgggg ccgacggcat ctggaaccgg accatcaaga acaagctcaa catggccgac
      781 tcgcttatga agcattgcat caagtatctc gagcaaaagg gctacatcgc cagcatgaag
      841 aacgtcgagc accccaacaa gaagatgtac atcaaggcga acctacgtcc ctccgatcgc
      901 gccacaggtg gcccctggtt tactgagggc gagctcgaca cggccttcat caacgagcta
      961 caggaaattg tgtttgactt tatcaagcag aaatcgacat atacttccca cggcggcggt
     1021 gctggagtac caggagtagg agcacaagga ggaggagcac agcagcgtga aaagaatccc
     1081 aagaagggcg tggttcaggg gaccattccc cactcggagg atatttccaa ggggacgaag
     1141 cgcagcgcgg gcgagatttc caacgacgac acggcggcac cggcaccggc caagaagacc
     1201 aggcccggca agccgcaatt gctgcctctc ccggccggtt acaagtcgta cccgactgtg
     1261 tcggacattg caaagttcat ccaccacgcc ggcatcacga ataatacgac gttgagcgag
     1321 gcggatatcc agcagttggt ggatgtgcta tactacgatg gcttagtaga gcctgtcaag
     1381 gtcaatgggc gcaaggggta ccgtgtgacg cgcatcccca aacaggatcc gaggacgtgc
     1441 aatcaacgac gcctcgacca tgatccaacc aaaggaggca ttgttacctc atttatggga
     1501 gttgagccga gtgcaagtgg ccttatggag gcgccttgtg ggcggtgccc tgtctatgat
     1561 atgtgcgagg aaggcggacc tgtcagcccc agcaactgcg tctatttcag acggtggctg
     1621 gatggtgacg aggtacagga acaagtcatt tcgatctgat cgactcgaat gtgtttgtgt
     1681 ctccgtttag aagacgttgc atggtctcta tcacaactgc attaacggcg taaaaaaaaa
     1741 gaaggcaagg gtaatttggt ttcatctagg cgttggaggt aatctgttta gcattcaatg
     1801 tgttggcgat aacattcaca tgacctgcaa actgtctagg tattctatag tgtcggatcc
     1861 tctagtcact tgtagagctc gtccatgccg ttgagcttgt ggccgagctt ggaggggagg
     1921 tcgcagtagc gggcgacggc gacctcgtgc tgctcgacgt aggtctcgtt gtcggcctcc
     1981 ttgatgcgct cgaggcggcg gtcgacgaag tagacgccgg gcatcttgag gttcttggcg
     2041 ggcttcttgg agcggtaggt ggtcttgagg ttgcagtgga ggtggccgcc gccgacgagc
     2101 ttgagggcca tgtcgcagcg gccctcgagg ccgccgtcgg cggggtagag ggtctcggtg
     2161 gaggcctccc agccgagggt cttcttctgc atgacggggc cgttggaggg gaagttgacg
     2221 ccgcggagct tgacgttgta gatgaggcag ccgtcctgga gggaggtgtc ctgggtgacg
     2281 gtgaggacgc cgccgtcctc gtaggtggtg acgcgctccc aggtgaagcc ctcggggaag
     2341 gactgcttga agaagtcggg gatgccctgg gtgtggttga tgaaggtctt ggagccgtac
     2401 atgaagcagg tggcgaggat gtcgaaggcg aaggggaggg ggccgccctc gacgaccttg
     2461 atgcggttgg tctgggtgcc ctcgtagggc ttgccctcgc cctcgtgggt gcacttgaag
     2521 tggtggttgt tgacggtgcc ctccatgtag agcttggtgt gcatgttctc cttgatgagc
     2581 tcctcgccct tggagaccat gccagcaata gccaaaccaa taccagccct cttcttaacg
     2641 ctcggacggg gcttgcactc gtcctgctgt actgggtgga gcatgacctt ggtcgggtac
     2701 gtgggcgagt tgccttggta ccccttgctc cagtcataac taatggcgta ggcaaagatg
     2761 ctgccggtct tgttgaacgt tgtcgaggtg atgctgccgc cgacgttggg gtaacccttg
     2821 agacggtgct tggcgtcctt gtcccagaag tggaaggtgc cgtcgctacc ggctgtgctg
     2881 aaggtgccgt gttgggggtg gaaggagatg tcgttgacag cgtggacggc cgtggtgttg
     2941 ccttgggcgg ggtcgcggtg gcatttgaaa gagaagttgg agctggaaaa caagtcagtt
     3001 agtcagcgta ttcggcagat gggatagtaa agggggagga ggtgaaggcg tacgcagaat
     3061 ccttgtcctc cacatattga atggcgcatc taccctcgat cgagccgata gcaaagccct
     3121 gagaatcgtt gaagcagctg accacacgag tctgccattt aagagggctc tgcattgtct
     3181 tgtagaactt gccggggtcc ttcaggttga tcacgttgat atagcgatca gcggttccga
     3241 ccacgagcaa gttgtcgcgc acatccatgg tataaacgcg ctcctgggcc tgcaccgtgg
     3301 cggccgggtt cgcggagcgg aaatcccagt acttgatctg cttgtcccag ccacctgtca
     3361 ccgccatggg cgtgccattg gcctcgaagt agcgaacgca cttgatgggc atatcgtgag
     3421 cagcgacctg catcgactga ccggtggcca aatcgagaac cttggcggcc ttgtcggcgc
     3481 cggcggaaat gacctttgtg ccgtcctgtt gggagaagtg catgtcagtg ttttgtcggg
     3541 ctacctgcca ggccaatcga cgcaaacata ccttgaagaa atcgcaagag aaaacggggg
     3601 catcatgctc aatcatggcc tgtccctgac cttgaccatt actcaagatc tcgtaaatgc
     3661 gtgtcttctt gtcccagctg gctacggcga ggaagtcctt ctggtcggcg gggttggggt
     3721 tgaaggcaag gtcgctgatg ctgtcctcgg ggggttggcc gagctcgaca tcctgcttaa
     3781 gatcgccgag ggtgttgctc gcggatgcgg cggcggaacc aaaaagacca gccatgatgg
     3841 atgctgatga tgatctcttc ctttcgaaaa gggagggaag tgacgtcttc ttatgtattt
     3901 ccaagatggg ttattgttga cgtctggtgg atcctgtcga cggcaggcct agctcatacc
     3961 tagcagcgga gtcctcgagc gtgagatggc gtggggaagc ggagactcca agaagggcca
     4021 aggcagggca gtgtgcagat cgggttgcgg agtttgaaca acgtatcaga tgtaagacga
     4081 gattgggagc acttttagtc gccagggggg aagagatgct ctgatgtgtt cagcgatcac
     4141 cctttctctg ttgttgatgg gcacgagtca ggtatatgta tgtatccttc tccccttctg
     4201 tccctttcgc atcagggtgt gtaggttggg taggtactgt agatagccaa tgtggaatgc
     4261 aacaccaggc tctcgttcat atgcaaaagc aggcctgtta acttcggcaa gccgtaagat
     4321 cattccttat ggtggcatgg agggcatctg agggcatccc atggtggggt gattcgtttg
     4381 atgggtggtc tcaccagctg tttattattc ccggttcacc ctccattgtc gtctcgtttg
     4441 cccaattctc tttgaccacc cactatcccc aggcgttcac tggctagtgc gcccttgttg
     4501 ccgtcgttcg attggccggg gattgtggtc aactgtgggg ggaccgcagc gccaagttca
     4561 agcgccaagc cgagggtcaa caccctcgtt acacaagccg tatgcccgga agcggccgcc
     4621 agctgcacgc accccagacg gctcctgcag aaagtgcgtg gccaccaccg cggccactga
     4681 cggactgcct gtgacacctg gctctctcgc tgtcaactgc catatgtaca tcgatcccta
     4741 cactgacact gagcctggcc ccaatgcgcc cagttctgga cacgatgaag ccacattcca
     4801 aaacctgcat gtgtgagaga aggtgcaagg aacggattgg cccggcgtct agggagatct
     4861 gtatggcctg cagtcaccta aatcgtataa ttccgcacct caatgtccat ctgatcccga
     4921 caagaccgtg aaacgtcgcc ctgaaagcga tcagcccgaa caaaaaccac caagtcgcca
     4981 cagcatcccg cccgtctggc aatcatcacc gcgaggctca gattggatct agagaagaat
     5041 taccttagcc catcagaaca attcccagca actccggcga tagacacgtt tacgcaatgg
     5101 atttcaatag cctcaaggat accgtgtcca acctgacact ctacgacatc aaggctggcg
     5161 tacgaaaggt tcaaaatggt aggtgaccac ctagtccaga accaagactc cctcatgatt
     5221 tcgtgttcag ggtgctgaca atggctccaa ccagctgtca tgaactatac cgagatggag
     5281 tccaaggtat gcccgtatcc acgcgccaca ctggcaccat tctcttgcgc ccttcgccat
     5341 atacttgatc ccagacaaga gaccatggaa cgggagctga cagcgaaccc aggtgcgcga
     5401 ggccaccaac aatgaacctt ggggtgcatc gtcgactttg atgcaagaga tcgccgatgg
     5461 caccttcaac tagtacgttc tcgagaactg ctcgcttgta tacgaacgcg gctaacttgc
     5521 gtttccctcc ttgttcttag ccaaacactc aacgaaatca tgcccatgat ttaccgccgt
     5581 ttcacagaaa agtccgccga agaatggcgc cagatctaca aggcccttca actactagag
     5641 tacctgatta agcacggttc cgaacgagtg gtggacgatg ctcggtcgca tcttaccctc
     5701 ctcaagatgc tgcgccagtt ccattttatc gaccagaacg gcaaagacca gggtatcaac
     5761 gtccgcaacc gcgccaagga actcgccgag ctcctcagcg atgtcgagcg tatccgagca
     5821 gagcgcaaga aggcccgcgc aaacaagggc aagttcacgg gcatttcggg tggaatgagc
     5881 ttctccagcg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5941 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6001 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6061 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6121 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6181 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6241 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6301 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6361 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6421 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     6481 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagggtaa
     6541 taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta
     6601 taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt
     6661 tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac
     6721 aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa
     6781 tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc
     6841 atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt
     6901 cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa
     6961 ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa
     7021 accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc
     7081 tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt
     7141 tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt
     7201 gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg
     7261 acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca
     7321 caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg
     7381 agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt
     7441 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     7501 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     7561 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     7621 tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7681 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7741 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7801 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7861 gttaatgtca tgataataat ggtttcttag tatgatccaa tatcaaagga aatgatagca
     7921 ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt
     7981 gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac
     8041 tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact
     8101 atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga
     8161 acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac
     8221 gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     8281 ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact
     8341 gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc
     8401 tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt
     8461 gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta
     8521 gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta
     8581 aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga
     8641 ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg
     8701 gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt
     8761 aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa
     8821 agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt
     8881 atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc
     8941 tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc
     9001 tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag
     9061 cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat
     9121 agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa
     9181 attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgttcagg
     9241 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9301 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9361 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9421 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9481 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9541 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9601 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9661 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9721 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9781 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9841 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9901 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9961 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    10021 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    10081 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10141 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10201 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10261 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10321 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10381 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10441 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10501 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10561 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10621 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10681 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10741 cagcttggag cgaac
//
