LOCUS       sequence-379330-       10956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_iRFP670
FEATURES             Location/Qualifiers
     source          1..10956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      124..1466
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(1087..1134)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1507..1525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1563..1585)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1685..1704
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1728..1943
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1944..2747
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(2878..3333)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3015..3036)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3227..3246
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             6260..6271
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6760..7695
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     primer_bind     complement(8940..8962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8951..8967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8975..8981,8982..8999,9000..9005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9020..9041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9158..9175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9329..9917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9409..9428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10088..10879,10880..10948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10711..10730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10949..10956,1..97))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
       61 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      121 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
      181 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
      241 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
      301 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
      361 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
      421 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
      481 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
      541 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
      601 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
      661 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
      721 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
      781 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
      841 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
      901 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
      961 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     1021 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     1081 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
     1141 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     1201 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     1261 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
     1321 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
     1381 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
     1441 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
     1501 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     1561 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     1621 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     1681 tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc aattcatcat
     1741 ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct
     1801 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
     1861 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
     1921 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
     1981 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
     2041 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
     2101 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
     2161 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
     2221 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
     2281 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
     2341 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
     2401 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
     2461 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
     2521 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
     2581 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
     2641 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
     2701 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
     2761 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
     2821 taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     2881 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2941 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3001 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3061 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3121 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3181 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3241 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3301 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     3361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3421 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gctggagaag
     3481 ctcattccac ccgaaatgcc cgtgaacttg cccttgtttg cgcgggcctt cttgcgctct
     3541 gctcggatac gctcgacatc gctgaggagc tcggcgagtt ccttggcgcg gttgcggacg
     3601 ttgataccct ggtctttgcc gttctggtcg ataaaatgga actggcgcag catcttgagg
     3661 agggtaagat gcgaccgagc atcgtccacc actcgttcgg aaccgtgctt aatcaggtac
     3721 tctagtagtt gaagggcctt gtagatctgg cgccattctt cggcggactt ttctgtgaaa
     3781 cggcggtaaa tcatgggcat gatttcgttg agtgtttggc taagaacaag gagggaaacg
     3841 caagttagcc gcgttcgtat acaagcgagc agttctcgag aacgtactag ttgaaggtgc
     3901 catcggcgat ctcttgcatc aaagtcgacg atgcacccca aggttcattg ttggtggcct
     3961 cgcgcacctg ggttcgctgt cagctcccgt tccatggtct cttgtctggg atcaagtata
     4021 tggcgaaggg cgcaagagaa tggtgccagt gtggcgcgtg gatacgggca taccttggac
     4081 tccatctcgg tatagttcat gacagctggt tggagccatt gtcagcaccc tgaacacgaa
     4141 atcatgaggg agtcttggtt ctggactagg tggtcaccta ccattttgaa cctttcgtac
     4201 gccagccttg atgtcgtaga gtgtcaggtt ggacacggta tccttgaggc tattgaaatc
     4261 cattgcgtaa acgtgtctat cgccggagtt gctgggaatt gttctgatgg gctaaggtaa
     4321 ttcttctcta gatccaatct gagcctcgcg gtgatgattg ccagacgggc gggatgctgt
     4381 ggcgacttgg tggtttttgt tcgggctgat cgctttcagg gcgacgtttc acggtcttgt
     4441 cgggatcaga tggacattga ggtgcggaat tatacgattt aggtgactgc aggccataca
     4501 gatctcccta gacgccgggc caatccgttc cttgcacctt ctctcacaca tgcaggtttt
     4561 ggaatgtggc ttcatcgtgt ccagaactgg gcgcattggg gccaggctca gtgtcagtgt
     4621 agggatcgat gtacatatgg cagttgacag cgagagagcc aggtgtcaca ggcagtccgt
     4681 cagtggccgc ggtggtggcc acgcactttc tgcaggagcc gtctggggtg cgtgcagctg
     4741 gcggccgctt ccgggcatac ggcttgtgta acgagggtgt tgaccctcgg cttggcgctt
     4801 gaacttggcg ctgcggtccc cccacagttg accacaatcc ccggccaatc gaacgacggc
     4861 aacaagggcg cactagccag tgaacgcctg gggatagtgg gtggtcaaag agaattgggc
     4921 aaacgagacg acaatggagg gtgaaccggg aataataaac agctggtgag accacccatc
     4981 aaacgaatca ccccaccatg ggatgccctc agatgccctc catgccacca taaggaatga
     5041 tcttacggct tgccgaagtt aacaggcctg cttttgcata tgaacgagag cctggtgttg
     5101 cattccacat tggctatcta cagtacctac ccaacctaca caccctgatg cgaaagggac
     5161 agaaggggag aaggatacat acatatacct gactcgtgcc catcaacaac agagaaaggg
     5221 tgatcgctga acacatcaga gcatctcttc ccccctggcg actaaaagtg ctcccaatct
     5281 cgtcttacat ctgatacgtt gttcaaactc cgcaacccga tctgcacact gccctgcctt
     5341 ggcccttctt ggagtctccg cttccccacg ccatctcacg ctcgaggact ccgctgctag
     5401 gtatgagcta ggcctgccgt cgacaggatc caccagacgt caacaataac ccatcttgga
     5461 aatacataag aagacgtcac ttccctccct tttcgaaagg aagagatcat catcagcatc
     5521 catcatggct ggtctttttg gttccgccgc cgcatccgcg agcaacaccc tcggcgatct
     5581 taagcaggat gtcgagctcg gccaaccccc cgaggacagc atcagcgacc ttgccttcaa
     5641 ccccaacccc gccgaccaga aggacttcct cgccgtagcc agctgggaca agaagacacg
     5701 catttacgag atcttgagta atggtcaagg tcagggacag gccatgattg agcatgatgc
     5761 ccccgttttc tcttgcgatt tcttcaaggt atgtttgcgt cgattggcct ggcaggtagc
     5821 ccgacaaaac actgacatgc acttctccca acaggacggc acaaaggtca tttccgccgg
     5881 cgccgacaag gccgccaagg ttctcgattt ggccaccggt cagtcgatgc aggtcgctgc
     5941 tcacgatatg cccatcaagt gcgttcgcta cttcgaggcc aatggcacgc ccatggcggt
     6001 gacaggtggc tgggacaagc agatcaagta ctgggatttc cgctccgaga acccggccgc
     6061 cacggtgcag gcccaggagc gcgtttatac catggatgtg cgcgacaact tgctcgtggt
     6121 cggaaccgct gatcgctata tcaacgtgat caacctgaag gaccccggca agttctacaa
     6181 gacaatgcag agccctctta aatggcagac tcgtgtggtc agctgcttca acgattctca
     6241 gggctttgct atcggctcga tcgagggtag atgcgccatt caatatgtgg aggacaagga
     6301 ttctgcgtac gccttcacct cctccccctt tactatccca tctgccgaat acgctgacta
     6361 actgacttgt tttccagctc caacttctct ttcaaatgcc accgcgaccc cgcccaaggc
     6421 aacaccacgg ccgtccacgc tgtcaacgac atctccttcc acccccaaca cggcaccttc
     6481 agcacagccg gtagcgacgg caccttccac ttctgggaca aggacgccaa gcaccgtctc
     6541 aagggttacc ccaacgtcgg cggcagcatc acctcgacaa cgttcaacaa gaccggcagc
     6601 atctttgcct acgccattag ttatgactgg agcaaggggt accaaggcaa ctcgcccacg
     6661 tacccgacca aggtcatgct ccacccagta cagcaggacg agtgcaagcc ccgtccgagc
     6721 gttaagaaga gggctggtat tggtttggct attgctggca tggcgcgtaa ggtcgatctc
     6781 acctcctgcg atcgcgagcc gatccacatc cccggcagca ttcagccgtg cggctgcctg
     6841 ctagcctgcg acgcgcaggc ggtgcggatc acgcgcatta cggaaaatgc cggcgcgttc
     6901 tttggacgcg aaactccgcg ggtcggtgag ctactcgccg attacttcgg cgagaccgaa
     6961 gcccatgcgc tgcgcaacgc actggcacag tcctccgatc caaagcgacc ggcgctgatc
     7021 ttcggttggc gcgacggcct gaccggccgc accttcgaca tctcactgca tcgccatgac
     7081 ggtacatcga tcatcgagtt cgagcctgcg gcggccgaac aggccgacaa tccgctgcgg
     7141 ctgacgcggc agatcatcgc gcgcaccaaa gaactgaagt cgctcgaaga gatggccgca
     7201 cgggtgccgc gctatctgca ggcgatgctc ggctatcacc gcgtgatgtt gtaccgcttc
     7261 gcggacgacg gctccgggat ggtgatcggc gaggcgaagc gcagcgacct cgagagcttt
     7321 ctcggtcagc actttccggc gtcgctggtc ccgcagcagg cgcggctact gtacttgaag
     7381 aacgcgatcc gcgtggtctc ggattcgcgc ggcatcagca gccggatcgt gcccgagcac
     7441 gacgcctccg gcgccgcgct cgatctgtcg ttcgcgcacc tgcgcagcat ctcgccctgc
     7501 catctcgaat ttctgcggaa catgggcgtc agcgcctcga tgtcgctgtc gatcatcatt
     7561 gacggcacgc tatggggatt gatcatctgt catcattacg agccgcgtgc cgtgccgatg
     7621 gcgcagcgcg tcgcggccga aatgttcgcc gacttcttat cgctgcactt caccgccgcc
     7681 caccaccaac gctaactaga ggatccgaca ctatagaata cctagacagt ttgcaggtca
     7741 tgtgaatgtt atcgccaaca cattgaatgc taaacagatt acctccaacg cctagatgaa
     7801 accaaattac ccttgccttc ttttttttta cgccgttaat gcagttgtga tagagaccat
     7861 gcaacgtctt ctaaacggag acacaaacac attcgagtcg atcagatcga aatgacttgt
     7921 tcctgtacct cgtcaccatc cagccaccgt ctgaaataga cgcagttgct ggggctgaca
     7981 ggtccgcctt cctcgcacat atcatagaca gggcaccgcc cacaaggcgc ctccataagg
     8041 ccacttgcac tcggctcaac tcccataaat gaggtaacaa tgcctccttt ggttggatca
     8101 tggtcgaggc gtcgttgatt gcacgtcctc ggatcctgtt tggggatgcg cgtcacacgg
     8161 taccccttgc gcccattgac cttgacaggc tctactaagc catcgtagta tagcacatcc
     8221 accaactgct ggatatccgc ctcgctcaac gtcgtattat tcgtgatgcc ggcgtggtgg
     8281 atgaactttg caatgtccga cacagtcggg tacgacttgt aaccggccgg gagaggcagc
     8341 aattgcggct tgccgggcct ggtcttcttg gccggtgccg gtgccgccgt gtcgtcgttg
     8401 gaaatctcgc ccgcgctgcg cttcgtcccc ttggaaatat cctccgagtg gggaatggtc
     8461 ccctgaacca cgcccttctt gggattcttt tcacgctgct gtgctcctcc tccttgtgct
     8521 cctactcctg gtactccagc accgccgccg tgggaagtat atgtcgattt ctgcttgata
     8581 aagtcaaaca caatttcctg tagctcgttg atgaaggccg tgtcgagctc gccctcagta
     8641 aaccaggggc cacctgtggc gcgatcggag ggacgtaggt tcgccttgat gtacatcttc
     8701 ttgttggggt gctcgacgtt cttcatgctg gcgatgtagc ccttttgctc gagatacttg
     8761 atgcaatgct tcataagcga gtcggccatg ttgagcttgt tcttgatggt ccggttccag
     8821 atgccgtcgg ccccggcttc gtcgaccatg gcgtatacca tcattatttc ggagttgggg
     8881 agggaggtgt accttttggg aggaagggga ggttagttgg tgtgctcggt tggctgtttc
     8941 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9001 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9061 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9121 ggagaggcgg tttgtgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9301 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10201 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10921 gggcgacacg gaaatgttga atactcatac tcttcc
//
