LOCUS       sequence-379332-       10602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Photoconvertible fluorescent nuclear reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_hH1_mEos3.1_V5
FEATURES             Location/Qualifiers
     source          1..10602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             195..998
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1129..1584)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1266..1287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1478..1497
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3666..3682
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3666..3682
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5548..5589
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6837..6859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6848..6864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6872..6878,6879..6896,6897..6902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6917..6938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7055..7072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7226..7814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7306..7325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7985..8776,8777..8845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8608..8627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8846..8950)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8977..10319
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9940..9987)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10360..10378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10416..10438)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10538..10557
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(10581..10602,1..194)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 attctttttt ttgatttcgg tttccttgaa atttttttga ttcggtaatc tccgaacaga
       61 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
      121 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga
      181 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
      241 ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat
      301 tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt
      361 gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc
      421 cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg
      481 acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg
      541 cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg
      601 cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca
      661 agggctccct atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca
      721 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
      781 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
      841 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
      901 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
      961 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
     1021 catgtatact aaactcacaa attagagctt caatttaatt atatcagtta ttaccctatg
     1081 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     1141 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1201 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     1261 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     1321 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     1381 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     1441 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1501 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     1561 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     1621 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     1681 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgctggagaa gctcattcca
     1741 cccgaaatgc ccgtgaactt gcccttgttt gcgcgggcct tcttgcgctc tgctcggata
     1801 cgctcgacat cgctgaggag ctcggcgagt tccttggcgc ggttgcggac gttgataccc
     1861 tggtctttgc cgttctggtc gataaaatgg aactggcgca gcatcttgag gagggtaaga
     1921 tgcgaccgag catcgtccac cactcgttcg gaaccgtgct taatcaggta ctctagtagt
     1981 tgaagggcct tgtagatctg gcgccattct tcggcggact tttctgtgaa acggcggtaa
     2041 atcatgggca tgatttcgtt gagtgtttgg ctaagaacaa ggagggaaac gcaagttagc
     2101 cgcgttcgta tacaagcgag cagttctcga gaacgtacta gttgaaggtg ccatcggcga
     2161 tctcttgcat caaagtcgac gatgcacccc aaggttcatt gttggtggcc tcgcgcacct
     2221 gggttcgctg tcagctcccg ttccatggtc tcttgtctgg gatcaagtat atggcgaagg
     2281 gcgcaagaga atggtgccag tgtggcgcgt ggatacgggc ataccttgga ctccatctcg
     2341 gtatagttca tgacagctgg ttggagccat tgtcagcacc ctgaacacga aatcatgagg
     2401 gagtcttggt tctggactag gtggtcacct accattttga acctttcgta cgccagcctt
     2461 gatgtcgtag agtgtcaggt tggacacggt atccttgagg ctattgaaat ccattgcgta
     2521 aacgtgtcta tcgccggagt tgctgggaat tgttctgatg ggctaaggta attcttctct
     2581 agatccaatc tgagcctcgc ggtgatgatt gccagacggg cgggatgctg tggcgacttg
     2641 gtggtttttg ttcgggctga tcgctttcag ggcgacgttt cacggtcttg tcgggatcag
     2701 atggacattg aggtgcggaa ttatacgatt taggtgactg caggtagaag gagcagtcca
     2761 tctgcgtgaa tcacgagaga atcagctact ttgaatcgat ggatgcagct accagaagtc
     2821 actcagttcg ttcaaagcca catcactggg cacttccatt gggacaggca ttgatcggac
     2881 gagaccgact tctggccgct ttcaacagcc acattatatc catgtcacgg ctacgcgcgg
     2941 ccttcggtaa cagaaaagca cacagacagc gattgtgata tggattcggg taaagtattg
     3001 gtggtcgcac catgtcatcc tgagtgtgca gtggctgcta ttcagatttc atctaactgc
     3061 gggagagggg ttcaaagggg cgtgacgtta tagacaacgg gtgaaggacg aagattgcct
     3121 cacttctttg ctagcaattt gcctgcaaag aaggcgcaca tgtcaagtaa acaactggga
     3181 aactactatt gaatacccac aatgcaaagc tcggaaggta cgtcttgatt gcagtgtgtc
     3241 gagtgtcaaa aaagaagcaa gtgttcatgc aagccaaaat tggcacctcc tccacttctc
     3301 cgagtgccct acccgaacct ccaggcgaga tggccggaat atactatccg cgttgggatt
     3361 atgacgtatc tccttcttct tcacatgatt ccatcccgtt gttgcttgtt tgcgagctgt
     3421 gacgggagat cgtagatgcc acttagggcc aggcaggcag tgcaggcagc caggaacaca
     3481 agcttccaac ttggtcatct cgattgccga ttaagggaac caaatgccta tataagactg
     3541 tcctcccacc tccccaatac cattcttttc ttcttccatc atcagccaac aaagcaatca
     3601 catcttcact acttcaaatc aacacaacac tcaaaccact ttcacaaccc ctcacatcaa
     3661 ccaaatctag aactagtgga tccaccatgc ctcccaagaa gaccgagacc aaggccgccg
     3721 atgcctctgc cgccgccgct cctgctcccg ccgctgctcc cacctctgct cccaagacca
     3781 agagccccag cacccatgcc tcttaccttg taagctttac ttaatcccca agagaaacgc
     3841 atcttttgct aacattcaaa caggacatga tcacggacgc tattgttgcg gtatttaaac
     3901 cccctcatta tctttgttgt ggcgtgtttt ggttgtgggc gcttctctgg tcggtgagca
     3961 aaatgagaca attgctctca tgatgcacgc aaccaagagt cacccacacc actcaccacc
     4021 cttttactgt ccctcagtcg tgctgattcc ttctttttgt catgctacag ctcaaggacc
     4081 gcgctggatc taggtatgtg ccatttttct gcttactttt ctcatttttt caccagggct
     4141 ttgccaagcc acaccgcaaa ccttgcgggc ccttcctacg gtcgcaccga tgaccacttt
     4201 cattcttatt tccatgacgc gatgaaggat cacaacatgt tgctaacgca caccacagcc
     4261 gtcaagctct caagaagtat gtccgtgcca acaacacgct cggcaacgtc accgacaaca
     4321 tgttcgactc gctcttcaac aaggccttga agaacggtgt cgacaagggt gtctttgagc
     4381 agcccaaggg tatgtcacca cccacatgac gcatcatctc cttgtattac tctttgcatg
     4441 ctcacacacg atataggtcc ttccggtggc accaagcttg ccaagaaggt cgctaagcct
     4501 gctcccaaga aggctgctcc caagaaggag accaaggaga agaagcccgc tgccgctaag
     4561 aaggagggtg ccgccaagaa ggagaccaag gagaagaagg ctcctgctgc caagaaggcg
     4621 gctgccccca agaaggctgc tgctcccaag aaggaggtca aggagaagaa ggcggctgcc
     4681 cccaagaaga aggccgctgc tcctgccgtt gccgacaagg agaccgtcct caccaagacc
     4741 aagtctggcc gtgtcgccaa gagcaccgct aagcctgctg ccgccaagaa ggccgctgct
     4801 cccaagaagg ctgccgccag caagaaggcc gagaaggccg agcccgctgc cgagaaggca
     4861 ttaattaaca tgtccgccat caagcccgac atgaagatca agctccgcat ggagggcaac
     4921 gtcaacggcc accacttcgt catcgacggc gacggcaccg gcaagccctt cgagggcaag
     4981 cagtccatgg acctcgaggt caaggagggc ggccccctcc ccttcgcctt cgacatcctc
     5041 accaccgcct tccactacgg caaccgcgtc ttcgccaagt accccgacaa catccaggac
     5101 tacttcaagc agtccttccc caagggctac tcctgggagc gctccctcac cttcgaggac
     5161 ggcggcatct gcaacgcccg caacgacatc accatggagg gcgacacctt ctacaacaag
     5221 gtccgcttct acggcaccaa cttccccgcc aacggccccg tcatgcagaa gaagaccctc
     5281 aagtgggagc cctccaccga gaagatgtac gtccgcgacg gcgtcctcac cggcgacgtc
     5341 gagatggccc tcctcctcga gggcaacgcc cactaccgct gcgacttccg caccacctac
     5401 aaggccaagg agaagggcgt caagctcccc ggcgcccact tcgtcgacca ctgcatcgag
     5461 atcctctccc acgacaagga ctacaacaag gtcaagctct acgagcacgc cgtcgcccac
     5521 tccggcctcc ccgacaacgc ccgccgcggt aagcctatcc ctaaccctct cctcggtctc
     5581 gattctacgt gactagagga tccgacacta tagaatacct agacagtttg caggtcatgt
     5641 gaatgttatc gccaacacat tgaatgctaa acagattacc tccaacgcct agatgaaacc
     5701 aaattaccct tgccttcttt ttttttacgc cgttaatgca gttgtgatag agaccatgca
     5761 acgtcttcta aacggagaca caaacacatt cgagtcgatc agatcgaaat gacttgttcc
     5821 tgtacctcgt caccatccag ccaccgtctg aaatagacgc agttgctggg gctgacaggt
     5881 ccgccttcct cgcacatatc atagacaggg caccgcccac aaggcgcctc cataaggcca
     5941 cttgcactcg gctcaactcc cataaatgag gtaacaatgc ctcctttggt tggatcatgg
     6001 tcgaggcgtc gttgattgca cgtcctcgga tcctgtttgg ggatgcgcgt cacacggtac
     6061 cccttgcgcc cattgacctt gacaggctct actaagccat cgtagtatag cacatccacc
     6121 aactgctgga tatccgcctc gctcaacgtc gtattattcg tgatgccggc gtggtggatg
     6181 aactttgcaa tgtccgacac agtcgggtac gacttgtaac cggccgggag aggcagcaat
     6241 tgcggcttgc cgggcctggt cttcttggcc ggtgccggtg ccgccgtgtc gtcgttggaa
     6301 atctcgcccg cgctgcgctt cgtccccttg gaaatatcct ccgagtgggg aatggtcccc
     6361 tgaaccacgc ccttcttggg attcttttca cgctgctgtg ctcctcctcc ttgtgctcct
     6421 actcctggta ctccagcacc gccgccgtgg gaagtatatg tcgatttctg cttgataaag
     6481 tcaaacacaa tttcctgtag ctcgttgatg aaggccgtgt cgagctcgcc ctcagtaaac
     6541 caggggccac ctgtggcgcg atcggaggga cgtaggttcg ccttgatgta catcttcttg
     6601 ttggggtgct cgacgttctt catgctggcg atgtagccct tttgctcgag atacttgatg
     6661 caatgcttca taagcgagtc ggccatgttg agcttgttct tgatggtccg gttccagatg
     6721 ccgtcggccc cggcttcgtc gaccatggcg tataccatca ttatttcgga gttggggagg
     6781 gaggtgtacc ttttgggagg aaggggaggt tagttggtgt gctcggttgg ctgtttcctg
     6841 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6901 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6961 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7021 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8881 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8941 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     9001 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     9061 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     9121 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     9181 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     9241 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     9301 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     9361 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     9421 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     9481 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     9541 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     9601 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     9661 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     9721 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     9781 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     9841 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     9901 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
     9961 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    10021 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    10081 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    10141 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    10201 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    10261 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    10321 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10381 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    10441 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10501 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    10561 agagtgcacc ataccacctt ttcaattcat catttttttt tt
//
