LOCUS       sequence-379333-       13443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Microtubule marker together with nuclear envelope marker driven by 
            bidirectional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 13443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
FEATURES             Location/Qualifiers
     source          1..13443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..116
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(154..176)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     276..295
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        319..534
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             535..1338
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1469..1924)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1606..1627)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1818..1837
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(3085..4020)
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             complement(4506..4517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      5249..5258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(10018..10040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10029..10045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10053..10059,10060..10077,10078..10083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10098..10119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10236..10253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10407..10995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10487..10506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11166..11957,11958..12026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11789..11808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12027..12131)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(12158..13443,1..57)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13121..13168)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata
       61 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      121 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      181 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      241 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
      301 agtgcaccat accacctttt caattcatca tttttttttt attctttttt ttgatttcgg
      361 tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag
      421 cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca
      481 gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg
      541 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
      601 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
      661 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
      721 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
      781 aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa
      841 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
      901 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
      961 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
     1021 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
     1081 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
     1141 ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat
     1201 gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
     1261 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
     1321 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
     1381 attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa taccgcacag
     1441 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     1501 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1561 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1621 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     1681 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     1741 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     1801 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     1861 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     1921 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1981 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     2041 cagggttttc ccagtcacga cgctggagaa gctcattcca cccgaaatgc ccgtgaactt
     2101 gcccttgttt gcgcgggcct tcttgcgctc tgctcggata cgctcgacat cgctgaggag
     2161 ctcggcgagt tccttggcgc ggttgcggac gttgataccc tggtctttgc cgttctggtc
     2221 gataaaatgg aactggcgca gcatcttgag gagggtaaga tgcgaccgag catcgtccac
     2281 cactcgttcg gaaccgtgct taatcaggta ctctagtagt tgaagggcct tgtagatctg
     2341 gcgccattct tcggcggact tttctgtgaa acggcggtaa atcatgggca tgatttcgtt
     2401 gagtgtttgg ctaagaacaa ggagggaaac gcaagttagc cgcgttcgta tacaagcgag
     2461 cagttctcga gaacgtacta gttgaaggtg ccatcggcga tctcttgcat caaagtcgac
     2521 gatgcacccc aaggttcatt gttggtggcc tcgcgcacct gggttcgctg tcagctcccg
     2581 ttccatggtc tcttgtctgg gatcaagtat atggcgaagg gcgcaagaga atggtgccag
     2641 tgtggcgcgt ggatacgggc ataccttgga ctccatctcg gtatagttca tgacagctgg
     2701 ttggagccat tgtcagcacc ctgaacacga aatcatgagg gagtcttggt tctggactag
     2761 gtggtcacct accattttga acctttcgta cgccagcctt gatgtcgtag agtgtcaggt
     2821 tggacacggt atccttgagg ctattgaaat ccattgcgta aacgtgtcta tcgccggagt
     2881 tgctgggaat tgttctgatg ggctaaggta attcttctct agatccaatc tgagcctcgc
     2941 ggtgatgatt gccagacggg cgggatgctg tggcgacttg gtggtttttg ttcgggctga
     3001 tcgctttcag ggcgacgttt cacggtcttg tcgggatcag atggacattg aggtgcggaa
     3061 ttatacgatt taggtgactg caggttagcg ttggtggtgg gcggcggtga agtgcagcga
     3121 taagaagtcg gcgaacattt cggccgcgac gcgctgcgcc atcggcacgg cacgcggctc
     3181 gtaatgatga cagatgatca atccccatag cgtgccgtca atgatgatcg acagcgacat
     3241 cgaggcgctg acgcccatgt tccgcagaaa ttcgagatgg cagggcgaga tgctgcgcag
     3301 gtgcgcgaac gacagatcga gcgcggcgcc ggaggcgtcg tgctcgggca cgatccggct
     3361 gctgatgccg cgcgaatccg agaccacgcg gatcgcgttc ttcaagtaca gtagccgcgc
     3421 ctgctgcggg accagcgacg ccggaaagtg ctgaccgaga aagctctcga ggtcgctgcg
     3481 cttcgcctcg ccgatcacca tcccggagcc gtcgtccgcg aagcggtaca acatcacgcg
     3541 gtgatagccg agcatcgcct gcagatagcg cggcacccgt gcggccatct cttcgagcga
     3601 cttcagttct ttggtgcgcg cgatgatctg ccgcgtcagc cgcagcggat tgtcggcctg
     3661 ttcggccgcc gcaggctcga actcgatgat cgatgtaccg tcatggcgat gcagtgagat
     3721 gtcgaaggtg cggccggtca ggccgtcgcg ccaaccgaag atcagcgccg gtcgctttgg
     3781 atcggaggac tgcgccagtg cgttgcgcag cgcatgggct tcggtctcgc cgaagtaatc
     3841 ggcaagtagc tcaccgaccc gcggagtttc gcgtccaaag aacgcgccgg cattttccgt
     3901 aatgcgcgtg atccgcaccg cctgcgcgtc gcaggctagc aggcagccgc acggctgaat
     3961 gctgccgggg atgtggatcg gctcgcgatc gcaggaggtg agatcgacct tacgcgccat
     4021 aagaatagca cccaagatag cacccctctt cttaacgctc ggacggggct tgcactcgtc
     4081 ctgctgtact gggtggagca tgaccttggt cgggtacgtg ggcgagttgc cttggtaccc
     4141 cttgctccag tcataactaa tggcgtaggc aaagatgctg ccggtcttgt tgaacgttgt
     4201 cgaggtgatg ctgccgccga cgttggggta acccttgaga cggtgcttgg cgtccttgtc
     4261 ccagaagtgg aaggtgccgt cgctaccggc tgtgctgaag gtgccgtgtt gggggtggaa
     4321 ggagatgtcg ttgacagcgt ggacggccgt ggtgttgcct tgggcggggt cgcggtggca
     4381 tttgaaagag aagttggagc tggaaaacaa gtcagttagt cagcgtattc ggcagatggg
     4441 atagtaaagg gggaggaggt gaaggcgtac gcagaatcct tgtcctccac atattgaatg
     4501 gcgcatctac cctcgatcga gccgatagca aagccctgag aatcgttgaa gcagctgacc
     4561 acacgagtct gccatttaag agggctctgc attgtcttgt agaacttgcc ggggtccttc
     4621 aggttgatca cgttgatata gcgatcagcg gttccgacca cgagcaagtt gtcgcgcaca
     4681 tccatggtat aaacgcgctc ctgggcctgc accgtggcgg ccgggttcgc ggagcggaaa
     4741 tcccagtact tgatctgctt gtcccagcca cctgtcaccg ccatgggcgt gccattggcc
     4801 tcgaagtagc gaacgcactt gatgggcata tcgtgagcag cgacctgcat cgactgaccg
     4861 gtggccaaat cgagaacctt ggcggccttg tcggcgccgg cggaaatgac ctttgtgccg
     4921 tcctgttggg agaagtgcat gtcagtgttt tgtcgggcta cctgccaggc caatcgacgc
     4981 aaacatacct tgaagaaatc gcaagagaaa acgggggcat catgctcaat catggcctgt
     5041 ccctgacctt gaccattact caagatctcg taaatgcgtg tcttcttgtc ccagctggct
     5101 acggcgagga agtccttctg gtcggcgggg ttggggttga aggcaaggtc gctgatgctg
     5161 tcctcggggg gttggccgag ctcgacatcc tgcttaagat cgccgagggt gttgctcgcg
     5221 gatgcggcgg cggaaccaaa aagaccagcc atggtggctt tgaagattgg gttcctcttt
     5281 gagagagtta atagacttga attgaggaat ggaaatcggg ctgagctcac tgctttatat
     5341 gtgagaaaag gtgatgagca ggtggtaaga ggcagatttt tgtggtcggt gctgtgtttt
     5401 gttgggtttg agctaggtgg ggtgagggag gggcaagcta aatagctacc agccctgagg
     5461 acgtcacaga gttttagtag ttctttttct gccatcggac atgcagctca cgcctagacc
     5521 caattacacc ctttgcgccc tcccacacat ccggccggtt ggatcattgt cagatacggc
     5581 agagaaatcg caacctcggc caagggtagc cacgattcga agccgcgaaa ttgggggttt
     5641 cggaatgtcg tcaagcggga accaatttga gtacccaatt cgccctattg gaatccatgg
     5701 aggagttctg tacgcgcaat tccgctctcc gtaaggatgc ttcggaggtg cacatggtct
     5761 catacatgta ggcccgacga ggatcgagtc ggttccgaag taggatcgtc tcgattgttg
     5821 ggcatcattg catggacatt cagagggcct actgatacct ggaatccgca ccgtccggct
     5881 acctagcaat aagattctgt gtatataaag ggctaaggtg tccgtccttg ataaaaccac
     5941 caccctcaac aacttacctc gactatcagc atcccgtcct atctaacaat cgtccatcgg
     6001 tatccaactc caactctatt cgcagggtcc tagaatcgta agtacacgct tatatcttgt
     6061 tgccagcgat agctgacaat gaatgaatat aggtcatgcg ttctatcgcc accatgcgtg
     6121 aaattgtaag tctccctctg atgcctactg tgccgcgcac gcagagcaat ccctcttttt
     6181 tttctctttc cctcccaaga gacccctgat tgctctaccc caccaaactg cctcgtcccc
     6241 ttctgtgcct ctacctactg ctgcccgact cattatgatc ctgctcgctt tctcgccatc
     6301 gcggactgcc atctatcgcg agtattatgg acatcgacgc tgacacgatt tcttcacgtg
     6361 ctataggttc atctccaaac cggccaatgc gtaagtcgat gatggcttcc acccccgtcg
     6421 cgatccaaca acaacagcag gactaacaca acagccacga tcagggtaac caaatcggtg
     6481 ctgctttctg gtacgtcgtc atcatcgcga acaaccacac ctttgagcga gggagcccga
     6541 cgctgacaga ataaacaggc agactatctc cggcgagcac ggcctcgatg cctccggtgt
     6601 gtacgtaacc tcgtgcccaa taaccatatc tggtgatggc ctcgaaagct caccgcccac
     6661 tacaggtaca atggcacctc tgagctccag ctcgagcgca tgaacgtcta cttcaacgag
     6721 gtgagcaaca accggtctcg acgatctccc cctcctcgag aattcgctcg ctaactagct
     6781 tcgcgcttga caggcttccg gcaacaagta tgtccctcgt gccgtcctcg tcgatctcga
     6841 gcccggtacc atggacgccg ttcgcgccgg tcccttcggc cagctcttcc gccccgataa
     6901 cttcgtcttc ggccagtccg gtgctggcaa caactgggcc aagggtcact acactgaggg
     6961 tgctgagctt gttgaccagg ttctcgatgt cgtccgtcgc gaggctgagg gctgcgactg
     7021 cctccagggc ttccagatca cccactccct cggtggtggt accggtgccg gtatgggtac
     7081 cctccttatc tccaagattc gtgaggagtt ccccgaccgc atgatggcta ccttctccgt
     7141 cgtgccctcc cccaaggtct ccgataccgt tgtcgagccc tacaacgcca ccctctccgt
     7201 ccatcagctc gttgagaact ccgacgagac cttctgcatt gacaacgagg cgctttacga
     7261 catttgcatg aggaccctca agctctccaa cccctcttac ggcgatctta accacctcgt
     7321 ctccgccgtc atgtccggtg tcaccgtctc cctccgtttc cccggccagc tgaactccga
     7381 tctccgcaag ctcgccgtca acatggttcc cttcccccgt ctccacttct tcatggtcgg
     7441 cttcgctccc cttaccagcc gcggcgccca ccacttccgt gccgtctccg tgcccgagtt
     7501 gacccagcag atgttcgacc ccaagaacat gatggctgct tctgacttcc gcaacggtcg
     7561 ttacctcacc tgctctgcca tcttgtaagt tgctgatcat ggaatgctgc ttttgaccac
     7621 taatactgac gaatcaaaca gccgtggcaa ggtctccatg aaggaggttg aggaccagat
     7681 gcgcaacgtt cagaacaaga actcttccta cttcgtcgag tggatcccca acaacgtcca
     7741 gactgccctc tgctctatcc ctccccgcgg tctcaagatg tcctccacct tcgtcggtaa
     7801 ctccaccgcc atccaggagc tcttcaagcg tatcggcgag cagttcactg ccatgttcag
     7861 gcgcaaggct ttcttgcatt ggtacactgg tgagggtatg gacgagatgg agttcactga
     7921 ggctgagtcc aacatgaacg atctcgtctc cgagtaccag cagtaccagg atgctggtgt
     7981 tgacgaggag gaggaggagt acgaggagga ggcccccctt gagggcgagg aggctggtat
     8041 tggtttggct attgctggca tggtctccaa gggcgaggag ctcatcaagg agaacatgca
     8101 catgaagctc tacatggagg gcaccgtcga caaccaccac ttcaagtgca cctccgaggg
     8161 cgagggcaag ccctacgagg gcacccagac catgcgcatc aaggtcgtcg agggcggccc
     8221 cctccccttc gccttcgaca tcctcgccac ctccttcctc tacggctcca agaccttcat
     8281 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccccgagg gcttcacctg
     8341 ggagcgcgtc accacctacg aggacggcgg cgtcctcacc gccacccagg acacctccct
     8401 ccaggacggc tgcctcatct acaacgtcaa gatccgcggc gtcaacttca cctccaacgg
     8461 ccccgtcatg cagaagaaga ccctcggctg ggaggccttc accgagaccc tctaccccgc
     8521 cgacggcggc ctcgagggcc gcaacgacat ggccctcaag ctcgtcggcg gctcccacct
     8581 catcgccaac gccaagacca cctaccgctc caagaagccc gccaagaacc tcaagatgcc
     8641 cggcgtctac tacgtcgact accgcctcga gcgcatcaag gaggccaaca acgagaccta
     8701 cgtcgagcag cacgaggtcg ccgtcgcccg ctactgcgac ctcccctcca agctcggcca
     8761 caagctcaac tgactagagg atccgacact atagaatacc tagacagttt gcaggtcatg
     8821 tgaatgttat cgccaacaca ttgaatgcta aacagattac ctccaacgcc tagatgaaac
     8881 caaattaccc ttgccttctt tttttttacg ccgttaatgc agttgtgata gagaccatgc
     8941 aacgtcttct aaacggagac acaaacacat tcgagtcgat cagatcgaaa tgacttgttc
     9001 ctgtacctcg tcaccatcca gccaccgtct gaaatagacg cagttgctgg ggctgacagg
     9061 tccgccttcc tcgcacatat catagacagg gcaccgccca caaggcgcct ccataaggcc
     9121 acttgcactc ggctcaactc ccataaatga ggtaacaatg cctcctttgg ttggatcatg
     9181 gtcgaggcgt cgttgattgc acgtcctcgg atcctgtttg gggatgcgcg tcacacggta
     9241 ccccttgcgc ccattgacct tgacaggctc tactaagcca tcgtagtata gcacatccac
     9301 caactgctgg atatccgcct cgctcaacgt cgtattattc gtgatgccgg cgtggtggat
     9361 gaactttgca atgtccgaca cagtcgggta cgacttgtaa ccggccggga gaggcagcaa
     9421 ttgcggcttg ccgggcctgg tcttcttggc cggtgccggt gccgccgtgt cgtcgttgga
     9481 aatctcgccc gcgctgcgct tcgtcccctt ggaaatatcc tccgagtggg gaatggtccc
     9541 ctgaaccacg cccttcttgg gattcttttc acgctgctgt gctcctcctc cttgtgctcc
     9601 tactcctggt actccagcac cgccgccgtg ggaagtatat gtcgatttct gcttgataaa
     9661 gtcaaacaca atttcctgta gctcgttgat gaaggccgtg tcgagctcgc cctcagtaaa
     9721 ccaggggcca cctgtggcgc gatcggaggg acgtaggttc gccttgatgt acatcttctt
     9781 gttggggtgc tcgacgttct tcatgctggc gatgtagccc ttttgctcga gatacttgat
     9841 gcaatgcttc ataagcgagt cggccatgtt gagcttgttc ttgatggtcc ggttccagat
     9901 gccgtcggcc ccggcttcgt cgaccatggc gtataccatc attatttcgg agttggggag
     9961 ggaggtgtac cttttgggag gaaggggagg ttagttggtg tgctcggttg gctgtttcct
    10021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10201 agaggcggtt tgtgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt
    12181 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
    12241 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
    12301 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
    12361 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
    12421 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
    12481 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
    12541 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
    12601 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
    12661 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
    12721 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
    12781 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
    12841 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
    12901 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
    12961 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
    13021 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
    13081 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
    13141 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
    13201 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
    13261 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
    13321 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
    13381 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
    13441 cca
//
