LOCUS       sequence-379371-        8201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane,
            e.g. for single molecule fluorescence microscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Sotolongo Bellon J, Birkholz O, Richter CP, Eull F, Kenneweg H, 
            Wilmes S, Rothbauer U, You C, Walter MR, Kurre R, Piehler J
  TITLE     Four-color single-molecule imaging with engineered tags resolves the
            molecular architecture of signaling complexes in the plasma 
            membrane.
  JOURNAL   Cell Rep Methods. 2022 Feb 4;2(2):100165. doi: 
            10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28.
  PUBMED    35474965
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-02-4"; volume: "2(2):100165. doi"; pages: " 
            10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28"
COMMENT     Sequence Label: pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..47)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        219..598
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        599..802
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     753..773
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     847..866
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        847..865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     902..961
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             965..991
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1001..1546
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             join(1568..1570,1571..1573,1574..2284)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDDHFLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1613..1634)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1874..1893)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2221..2242
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          3961..4190
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4173..4192
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4263..4837,4838..4840,4841..4849)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4430..4447)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4657..4676
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4872..5675
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4935..4954)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5545..5564
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5805..5926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5842..5861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6028..6044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6028..6044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6041..6063)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6052..6068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6076..6082,6083..6100,6101..6106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6121..6142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6259..6276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6430..7018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6510..6529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7189..7980,7981..8049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7812..7831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8050..8154)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
       61 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      121 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      181 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      601 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      661 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      721 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      781 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      841 cgaaattaat acgactcact atagggagac ccaagcttgg taccgagctc ggatcgatat
      901 catggagaca gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg
      961 tgactatcca tatgatgttc cagattatgc tgaattcacc atggacaaag actgcgaaat
     1021 gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt gcgaacaggg
     1081 cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc
     1141 tgccccagcc gccgtgctgg gcggaccaga gccactgatg caggccaccg cctggctcaa
     1201 cgcctacttt caccagcctg aggccatcga ggagttccct gtgccagccc tgcaccaccc
     1261 agtgttccag caggagagct ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa
     1321 gttcggagag gtcatcagct acagccacct ggccgccctg gccggcaatc ccgccgccac
     1381 cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc cctgccaccg
     1441 ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc gggctcgccg tgaaagagtg
     1501 gctgctggcc cacgagggcc acagactggg caagcctggg ctgggtcctg caggtatagg
     1561 cgcgccaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1621 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1681 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1741 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1801 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1861 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1921 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1981 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     2041 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     2101 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2161 cgatcacttc ctgagcaccc agtccaaact gagcaaagac cccaacgaga agcgcgatca
     2221 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2281 caagggatcc ctcgagtcta aaaatctaaa atctcctcaa aaagtagagg tcgacatcat
     2341 agatgacaac tttatcctga ggtggaacag gagcgatgag tctgtcggga atgtgacttt
     2401 ttcattcgat tatcaaaaaa ctgggatgga taattggata aaattgtctg ggtgtcagaa
     2461 tattactagt accaaatgca acttttcttc actcaagctg aatgtttatg aagaaattaa
     2521 attgcgtata agagcagaaa aagaaaacac ttcttcatgg tatgaggttg actcatttac
     2581 accatttcgc aaagctcaga ttggtcctcc agaagtacat ttagaagctg aagataaggc
     2641 aatagtgata cacatctctc ctggaacaaa agatagtgtt atgtgggctt tggatggttt
     2701 aagctttaca tatagcttac ttatctggaa aaactcttca ggtgtagaag aaaggattga
     2761 aaatatttat tccagacata aaatttataa actctcacca gagactactt attgtctaaa
     2821 agttaaagca gcactactta cgtcatggaa aattggtgtc tatagtccag tacattgtat
     2881 aaagaccaca gttgaaaatg aactacctcc accagaaaat atagaagtca gtgtccaaaa
     2941 tcagaactat gttcttaaat gggattatac atatgcaaac atgacctttc aagttcagtg
     3001 gctccacgcc tttttaaaaa ggaatcctgg aaaccatttg tataaatgga aacaaatacc
     3061 tgactgtgaa aatgtcaaaa ctacccagtg tgtctttcct caaaacgttt tccaaaaagg
     3121 aatttacctt ctccgcgtac aagcatctga tggaaataac acatcttttt ggtctgaaga
     3181 gataaagttt gatactgaaa tacaagcttt cctacttcct ccagtcttta acattagatc
     3241 ccttagtgat tcattccata tctatatcgg tgctccaaaa cagtctggaa acacgcctgt
     3301 gatccaggat tatccactga tttatgaaat tattttttgg gaaaacactt caaatgctga
     3361 gagaaaaatt atcgagaaaa aaactgatgt tacagttcct aatttgaaac cactgactgt
     3421 atattgtgtg aaagccagag cacacaccat ggatgaaaag ctgaataaaa gcagtgtttt
     3481 tagtgacgct gtatgtgaga aaacaaaacc aggaaatacc tctaaaattt ggcttatagt
     3541 tggaatttgt attgcattat ttgctctccc gtttgtcatt tatgctgcga aagtcttctt
     3601 gagatgcatc aattatgtct tctttccatc acttaaacct tcttccagta tagatgagta
     3661 tttctctgaa cagccattga agaatcttct gctttcaact tctgaggaac aaatcgaaaa
     3721 atgtttcata attgaaaata taagcacaat tgctacagta gaagaaacta atcaaactga
     3781 tgaagatcat aaaaaataca gttcccaaac tagccaagat tcaggaaatt attctaatga
     3841 agatgaaagc gaaagtaaaa caagtgaaga actacagcag gactttgtat gagcggccgc
     3901 atagataact gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
     3961 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
     4021 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
     4081 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
     4141 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
     4201 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc
     4261 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4321 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4381 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4441 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     4501 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     4561 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     4621 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     4681 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     4741 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     4801 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc gggataattc
     4861 ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     4921 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4981 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5041 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     5101 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5161 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5221 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5281 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5341 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5401 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     5461 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5521 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5581 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5641 tcgccttcta tcgccttctt gacgagttct tctgagggga tcaattctct agataactga
     5701 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     5761 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     5821 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5881 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtcgagtgca
     5941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6001 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6061 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6241 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6841 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7081 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7141 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7201 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7261 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7321 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7381 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7441 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7501 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7561 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7621 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7681 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7741 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7801 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7861 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7921 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7981 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8041 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8101 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8161 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat c
//
