LOCUS       sequence-379373-        8420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid encoding myc-tagged OGA(GS-544)C181_NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Ge Y, Lu H, Yang B, Woo CM
  TITLE     Small Molecule-Activated O-GlcNAcase for Spatiotemporal Removal of 
            O-GlcNAc in Live Cells.
  JOURNAL   ACS Chem Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894.
  PUBMED    36598936
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894."
COMMENT     Sequence Label: pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(88..107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        279..658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        659..862
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     813..833
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     907..926
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        907..925
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             963..992
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3972..4019
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4055..4072)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4061..4285
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4331..4759
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4418..4437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4628..4649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4768..4788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4773..5102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4953..5088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5015..5034
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5169..5963
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5223..5242)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5833..5852
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6137..6258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6174..6193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6228..6247
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6307..6323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6307..6323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6320..6342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6331..6347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6355..6361,6362..6379,6380..6385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6400..6421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6538..6555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6709..7297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6789..6808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7468..8259,8260..8328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8091..8110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8329..8420,1..13))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
       61 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      121 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      181 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      241 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      301 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      361 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      421 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      481 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      541 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      601 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      661 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      721 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      781 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      841 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      901 cgaaattaat acgactcact atagggagac ccaagctggc gagcgtttaa gcttgagcaa
      961 tggagcagaa gctgatcagc gaggaggatc tggcgatcgc aatggtgcag aaggagagtc
     1021 aagcgacgtt ggaggagcgg gagagcgagc tcagctccaa ccctgccgcc tctgcggggg
     1081 catcgctgga gccgccggca gctccggcac ccggagaaga caaccccgcc ggggctgggg
     1141 gagcggcggt ggccggggcc gcaggagggg ctcggcggtt cctctgcggt gtggtggaag
     1201 gattttatgg aagaccttgg gttatggaac agagaaaaga actctttaga aggctccaga
     1261 aatgggaatt aaatacatac ttgtatgccc caaaagatga ctacaaacat aggatgtttt
     1321 ggcgagagat gtattcagtg gaggaagctg agcaacttat gactctcatc tctgctgcac
     1381 gagaatatga gatagagttc atctatgcga tctcacctgg attggatatc actttttcta
     1441 accccaagga agtatccaca ttgaaacgta aattggacca ggtttctcag tttgggtgca
     1501 gatcatttgc tttgcttttt gatgatatag accataatat gtgccttgcc gagggtaccc
     1561 gaatcttcga tccggtcact ggtacaacgc atcgcatcga ggatgttgtc gatgggcgca
     1621 agcctattca tgtcgtggct gctgccaagg acggaacgct gctcgcgcgg cccgtggtgt
     1681 cctggttcga ccagggaacg cgggatgtga tcgggttgcg gatcgccggt ggcgccatcg
     1741 tgtgggcgac acccgatcac aaggtgctga cagagtacgg ctggcgtgcc gccggggaac
     1801 tccgcaaggg agacagggtg gccggaccgg gtggttctgg taacagcctg gccttgtccc
     1861 tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg
     1921 agtatgatcc taccagtccc ttcagtgaag cttcgatgat gggcttactg accaacctgg
     1981 cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt
     2041 tgaccctcca tgatcaggcc caccttctag aacgtgcctg gctagagatc ctgatgattg
     2101 gtctcgtctg gcgctccatg gagcacccag ggaagctact gtttgctcct aacttgctct
     2161 tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg
     2221 ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat
     2281 ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg
     2341 aagagaagga ccatatccac cgagccctgg acaagatcac ggacactttg atccacctga
     2401 tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca
     2461 tcctctccca catcaggcac atgagtaaca aaggaatgga gcatctgtac agcatgaagt
     2521 acaagaacgt ggtgcccctc tatgacctgc tgctggagat gctggacgcc caccgcctac
     2581 atgcgggtgg ttctggtgct agccgcgtgc aggcgttcgc ggatgccctg gatgacaaat
     2641 tcctgcacga catgctggcg gaaggactcc gctattccgt gatccgagaa gtgctgccaa
     2701 cgcggcgggc acgaacgttc gacctcgagg tcgaggaact gcacaccctc gtcgccgaag
     2761 gggttgtcgt gcacaactgc gcagcagaca aagaggtatt cagttctttt gctcatgccc
     2821 aagtctccat cacaaatgaa atctatcagt acctaggaga gccagaaact ttcctcttct
     2881 gtcccacaga atactgtggc actttctgtt atccaaatgt gtctcagtct ccatatttaa
     2941 ggactgtggg tgaaaagctt ctacctggaa ttgaagtgct ttggacaggt cccaaagttg
     3001 tttctaaaga aattccagta gagtccatcg aagaggtttc taagattatt aagagagctc
     3061 cagtaatctg ggataacatt catgctaatg attatgatca gaagagactg tttctgggcc
     3121 cgtacaaagg aagatccaca gaactcatcc cacggttaaa aggagtcctc actaatccaa
     3181 attgtgaatt tgaagccaac tacgttgcta tccacaccct tgccacctgg tacaaatcaa
     3241 acatgaatgg agtgagaaaa gatgtagtga tgactgacag tgaagatagt actgtgtcca
     3301 tccagataaa attagaaaat gaaggcagtg atgaagatat tgaaactgat gtactctata
     3361 gtccacagat ggctctaaag ctagcattaa cagaatggtt gcaagagttt ggtgtgcctc
     3421 atcaatacag cagtagggga agcggctccg gaagtggatc tggaagtgga tccggaagcg
     3481 gagaaaagcc tttgtacact gcggaaccag tgaccctgga ggatttgcag ttacttgctg
     3541 atctattcta ccttccttac gagcatggac ccaaaggagc acagatgtta cgggaatttc
     3601 aatggcttcg agcaaatagt agtgttgtca gtgtcaattg caaaggaaaa gactctgaaa
     3661 aaattgaaga atggcggtca cgagcagcca agtttgaaga gatgtgtgga ctagtgatgg
     3721 gaatgttcac tcggctctcc aattgtgcca acaggacaat tctttatgac atgtactcct
     3781 atgtttggga tatcaagagt ataatgtcta tggtgaagtc ttttgtacag tggttagggt
     3841 gtcgtagtca ttcttcagca caattcttaa ttggagacca agaaccctgg gcctttagag
     3901 gtggtctagc aggagagttc cagcgtttgc tgccaattga tggggcaaat gatctctttt
     3961 ttcagccacc taagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaat
     4021 aagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4081 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4141 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4201 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4261 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4321 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4741 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4801 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4861 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4921 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4981 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5041 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5101 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5161 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5221 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5281 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5341 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5401 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5461 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5521 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5581 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5641 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5701 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5761 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5821 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5881 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5941 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6001 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6061 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6121 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6241 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7141 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8401 tatttagaaa aataaacaaa
//
