LOCUS       sequence-379374-        7136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid encoding myc-tagged OGA(GS-544).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ge Y, Lu H, Yang B, Woo CM
  TITLE     Small Molecule-Activated O-GlcNAcase for Spatiotemporal Removal of 
            O-GlcNAc in Live Cells.
  JOURNAL   ACS Chem Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894.
  PUBMED    36598936
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894."
COMMENT     Sequence Label: pcDNA3.1(+)-myc-OGA(GS-544)
FEATURES             Location/Qualifiers
     source          1..7136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(127..146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     946..965
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        946..964
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1002..1031
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2810..2827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2816..3040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3086..3514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3173..3192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3383..3404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3523..3543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3528..3857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3708..3843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3770..3789
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3924..4718
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3978..3997)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4588..4607
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4892..5013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4929..4948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4983..5002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5062..5078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5062..5078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5075..5097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5086..5102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5110..5116,5117..5134,5135..5140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5155..5176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5293..5310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5464..6052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5544..5563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6223..7014,7015..7083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6846..6865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7084..7136,1..52))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
       61 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
      121 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
      181 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
      241 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      301 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      841 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
      961 tagggagacc caagctggcg agcgtttaag cttgagcaat ggagcagaag ctgatcagcg
     1021 aggaggatct ggcgatcgca atggtgcaga aggagagtca agcgacgttg gaggagcggg
     1081 agagcgagct cagctccaac cctgccgcct ctgcgggggc atcgctggag ccgccggcag
     1141 ctccggcacc cggagaagac aaccccgccg gggctggggg agcggcggtg gccggggccg
     1201 caggaggggc tcggcggttc ctctgcggtg tggtggaagg attttatgga agaccttggg
     1261 ttatggaaca gagaaaagaa ctctttagaa ggctccagaa atgggaatta aatacatact
     1321 tgtatgcccc aaaagatgac tacaaacata ggatgttttg gcgagagatg tattcagtgg
     1381 aggaagctga gcaacttatg actctcatct ctgctgcacg agaatatgag atagagttca
     1441 tctatgcgat ctcacctgga ttggatatca ctttttctaa ccccaaggaa gtatccacat
     1501 tgaaacgtaa attggaccag gtttctcagt ttgggtgcag atcatttgct ttgctttttg
     1561 atgatataga ccataatatg tgtgcagcag acaaagaggt attcagttct tttgctcatg
     1621 cccaagtctc catcacaaat gaaatctatc agtacctagg agagccagaa actttcctct
     1681 tctgtcccac agaatactgt ggcactttct gttatccaaa tgtgtctcag tctccatatt
     1741 taaggactgt gggtgaaaag cttctacctg gaattgaagt gctttggaca ggtcccaaag
     1801 ttgtttctaa agaaattcca gtagagtcca tcgaagaggt ttctaagatt attaagagag
     1861 ctccagtaat ctgggataac attcatgcta atgattatga tcagaagaga ctgtttctgg
     1921 gcccgtacaa aggaagatcc acagaactca tcccacggtt aaaaggagtc ctcactaatc
     1981 caaattgtga atttgaagcc aactacgttg ctatccacac ccttgccacc tggtacaaat
     2041 caaacatgaa tggagtgaga aaagatgtag tgatgactga cagtgaagat agtactgtgt
     2101 ccatccagat aaaattagaa aatgaaggca gtgatgaaga tattgaaact gatgtactct
     2161 atagtccaca gatggctcta aagctagcat taacagaatg gttgcaagag tttggtgtgc
     2221 ctcatcaata cagcagtagg ggaagcggct ccggaagtgg atctggaagt ggatccggaa
     2281 gcggagaaaa gcctttgtac actgcggaac cagtgaccct ggaggatttg cagttacttg
     2341 ctgatctatt ctaccttcct tacgagcatg gacccaaagg agcacagatg ttacgggaat
     2401 ttcaatggct tcgagcaaat agtagtgttg tcagtgtcaa ttgcaaagga aaagactctg
     2461 aaaaaattga agaatggcgg tcacgagcag ccaagtttga agagatgtgt ggactagtga
     2521 tgggaatgtt cactcggctc tccaattgtg ccaacaggac aattctttat gacatgtact
     2581 cctatgtttg ggatatcaag agtataatgt ctatggtgaa gtcttttgta cagtggttag
     2641 ggtgtcgtag tcattcttca gcacaattct taattggaga ccaagaaccc tgggccttta
     2701 gaggtggtct agcaggagag ttccagcgtt tgctgccaat tgatggggca aatgatctct
     2761 tttttcagcc accttaagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     2821 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2881 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2941 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3001 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3061 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3481 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     3541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3781 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3841 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     3901 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     3961 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4021 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4081 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4141 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4201 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4261 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4321 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4381 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4441 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4501 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4561 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4621 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4681 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     4741 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     4801 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4861 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4921 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4981 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     5041 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatga
//
