LOCUS       sequence-379375-        8372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid encoding myc-tagged OGA(GS-544)C181-D174N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Ge Y, Lu H, Yang B, Woo CM
  TITLE     Small Molecule-Activated O-GlcNAcase for Spatiotemporal Removal of 
            O-GlcNAc in Live Cells.
  JOURNAL   ACS Chem Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894.
  PUBMED    36598936
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894."
COMMENT     Sequence Label: pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        295..674
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        675..878
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     829..849
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     923..942
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        923..941
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             979..1008
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4023..4040)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4029..4253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4299..4727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4386..4405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4596..4617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4736..4756)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4741..5070
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4921..5056
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4983..5002
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5137..5931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5191..5210)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5801..5820
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6105..6226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6142..6161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6196..6215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6275..6291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6275..6291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6288..6310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6299..6315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6323..6329,6330..6347,6348..6353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6368..6389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6506..6523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6677..7265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6757..6776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7436..8227,8228..8296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8059..8078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8297..8372,1..29))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
       61 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
      121 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      181 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      241 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      301 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      361 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      421 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      481 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      541 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      601 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      661 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      721 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      781 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      841 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      901 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggcgagc
      961 gtttaagctt gagcaatgga gcagaagctg atcagcgagg aggatctggc gatcgcaatg
     1021 gtgcagaagg agagtcaagc gacgttggag gagcgggaga gcgagctcag ctccaaccct
     1081 gccgcctctg cgggggcatc gctggagccg ccggcagctc cggcacccgg agaagacaac
     1141 cccgccgggg ctgggggagc ggcggtggcc ggggccgcag gaggggctcg gcggttcctc
     1201 tgcggtgtgg tggaaggatt ttatggaaga ccttgggtta tggaacagag aaaagaactc
     1261 tttagaaggc tccagaaatg ggaattaaat acatacttgt atgccccaaa agatgactac
     1321 aaacatagga tgttttggcg agagatgtat tcagtggagg aagctgagca acttatgact
     1381 ctcatctctg ctgcacgaga atatgagata gagttcatct atgcgatctc acctggattg
     1441 gatatcactt tttctaaccc caaggaagta tccacattga aacgtaaatt ggaccaggtt
     1501 tctcagtttg ggtgcagatc atttgctttg ctttttaatg atatagacca taatatgtgc
     1561 cttgccgagg gtacccgaat cttcgatccg gtcactggta caacgcatcg catcgaggat
     1621 gttgtcgatg ggcgcaagcc tattcatgtc gtggctgctg ccaaggacgg aacgctgctc
     1681 gcgcggcccg tggtgtcctg gttcgaccag ggaacgcggg atgtgatcgg gttgcggatc
     1741 gccggtggcg ccatcgtgtg ggcgacaccc gatcacaagg tgctgacaga gtacggctgg
     1801 cgtgccgccg gggaactccg caagggagac agggtggccg gaccgggtgg ttctggtaac
     1861 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
     1921 cccatactct attccgagta tgatcctacc agtcccttca gtgaagcttc gatgatgggc
     1981 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
     2041 ccaggctttg tggatttgac cctccatgat caggcccacc ttctagaacg tgcctggcta
     2101 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagggaa gctactgttt
     2161 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
     2221 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
     2281 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
     2341 accctgaagt ctctggaaga gaaggaccat atccaccgag ccctggacaa gatcacggac
     2401 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
     2461 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg aatggagcat
     2521 ctgtacagca tgaagtacaa gaacgtggtg cccctctatg acctgctgct ggagatgctg
     2581 gacgcccacc gcctacatgc gggtggttct ggtgctagcc gcgtgcaggc gttcgcggat
     2641 gccctggatg acaaattcct gcacgacatg ctggcggaag gactccgcta ttccgtgatc
     2701 cgagaagtgc tgccaacgcg gcgggcacga acgttcgacc tcgaggtcga ggaactgcac
     2761 accctcgtcg ccgaaggggt tgtcgtgcac aactgcgcag cagacaaaga ggtattcagt
     2821 tcttttgctc atgcccaagt ctccatcaca aatgaaatct atcagtacct aggagagcca
     2881 gaaactttcc tcttctgtcc cacagaatac tgtggcactt tctgttatcc aaatgtgtct
     2941 cagtctccat atttaaggac tgtgggtgaa aagcttctac ctggaattga agtgctttgg
     3001 acaggtccca aagttgtttc taaagaaatt ccagtagagt ccatcgaaga ggtttctaag
     3061 attattaaga gagctccagt aatctgggat aacattcatg ctaatgatta tgatcagaag
     3121 agactgtttc tgggcccgta caaaggaaga tccacagaac tcatcccacg gttaaaagga
     3181 gtcctcacta atccaaattg tgaatttgaa gccaactacg ttgctatcca cacccttgcc
     3241 acctggtaca aatcaaacat gaatggagtg agaaaagatg tagtgatgac tgacagtgaa
     3301 gatagtactg tgtccatcca gataaaatta gaaaatgaag gcagtgatga agatattgaa
     3361 actgatgtac tctatagtcc acagatggct ctaaagctag cattaacaga atggttgcaa
     3421 gagtttggtg tgcctcatca atacagcagt aggggaagcg gctccggaag tggatctgga
     3481 agtggatccg gaagcggaga aaagcctttg tacactgcgg aaccagtgac cctggaggat
     3541 ttgcagttac ttgctgatct attctacctt ccttacgagc atggacccaa aggagcacag
     3601 atgttacggg aatttcaatg gcttcgagca aatagtagtg ttgtcagtgt caattgcaaa
     3661 ggaaaagact ctgaaaaaat tgaagaatgg cggtcacgag cagccaagtt tgaagagatg
     3721 tgtggactag tgatgggaat gttcactcgg ctctccaatt gtgccaacag gacaattctt
     3781 tatgacatgt actcctatgt ttgggatatc aagagtataa tgtctatggt gaagtctttt
     3841 gtacagtggt tagggtgtcg tagtcattct tcagcacaat tcttaattgg agaccaagaa
     3901 ccctgggcct ttagaggtgg tctagcagga gagttccagc gtttgctgcc aattgatggg
     3961 gcaaatgatc tcttttttca gccaccttaa gtctagaggg cccgtttaaa cccgctgatc
     4021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4741 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5101 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5161 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5221 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5281 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5341 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5401 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5461 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5521 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5581 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5641 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5701 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5761 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5821 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5881 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5941 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6001 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6061 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6241 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8341 gtctcatgag cggatacata tttgaatgta tt
//
