LOCUS       sequence-37938-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Kim ST, Xu B, Kastan MB
  TITLE     Involvement of the cohesin protein, Smc1, in Atm-dependent and 
            independent responses to DNA damage.
  JOURNAL   Genes Dev. 2002 Mar 1;16(5):560-70.
  PUBMED    11877376
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2002-03-1"; volume: "16(5)"; pages: "560-70"
COMMENT     Sequence Label: pCDNA3 -5'cMyc-SMCl- S957A
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..104
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tggcttatcg aaattaatac gactcactat agggagaccc aagctccgcg ggtttaaacg
       61 gatccgccac catggaacag aaactcatct ctgaagagga tctggaattc atggggttcc
      121 tgaaactgat tgagattgag aactttaagt cgtacaaggg tcgacagatt atcggaccat
      181 ttcagaggtt caccgccatc attggaccca atggctctgg taagtcaaat ctcatggatg
      241 ccatcagctt tgtgctaggt gaaaaaacca gcaacctgcg ggtaaagacc ctgcgggacc
      301 tgatccatgg agctcctgtg ggcaagccag ctgccaaccg ggcctttgtc agcatggtct
      361 actctgagga gggtgctgag gaccgtacct ttgcccgtgt cattgtagga ggttcttctg
      421 agtacaagat caacaacaaa gtggtccaac tacatgagta cagtgaggaa ttagagaagt
      481 tgggcattct catcaaagct cgtaacttcc tcgttttcca gggtgctgtg gaatctattg
      541 ccatgaagaa ccccaaagag aggacagctc tatttgaaga gattagtcgt tctggggagc
      601 tggcgcagga gtatgacaag cgaaagaagg aaatggtgaa ggctgaagag gacacacagt
      661 ttaattacca tcgcaagaaa aatattgcgg ctgaacgcaa ggaagcaaag caggagaaag
      721 aagaggctga ccggtaccag cgcctgaagg atgaggtagt acgggctcag gtacagc
//
