LOCUS       sequence-37939-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Kim ST, Xu B, Kastan MB
  TITLE     Involvement of the cohesin protein, Smc1, in Atm-dependent and 
            independent responses to DNA damage.
  JOURNAL   Genes Dev. 2002 Mar 1;16(5):560-70.
  PUBMED    11877376
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2002-03-1"; volume: "16(5)"; pages: "560-70"
COMMENT     Sequence Label: pCDNA3 5' cMyc- SMCI-S966A
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
       61 ggagacccaa gctccgcggg tttaaacgga tccgccacca tggaacagaa actcatctct
      121 gaagaggatc tggaattcat ggggttcctg aaactgattg agattgagaa ctttaagtcg
      181 tacaagggtc gacagattat cggaccattt cagaggttca ccgccatcat tggacccaat
      241 ggctctggta agtcaaatct catggatgcc atcagctttg tgctaggtga aaaaaccagc
      301 aacctgcggg taaagaccct gcgggacctg atccatggag ctcctgtggg caagccagct
      361 gccaaccggg cctttgtcag catggtctac tctgaggagg gtgctgagga ccgtaccttt
      421 gcccgtgtca ttgtaggagg ttcttctgag tacaagatca acaacaaagt ggtccaacta
      481 catgagtaca gtgaggaatt agagaagttg ggcattctca tcaaagctcg taacttcctc
      541 gttttccagg gtgctgtgga atctattgcc atgaagaacc ccaaagagag gacagctcta
      601 tttgaagaga ttagtcgttc tggggagctg gcgcaggagt atgacaagcg aaagaaggaa
      661 atggtgaagg ctgaagagga cacacagttt aattaccatc gcaagaaaaa tattgcggct
      721 gaacgcaagg aagcaaagca ggagaaagaa gaggctgacc ggtaccagcg cctgaaggat
      781 gaggtagtac gggctcaggt acagctgcag ctc
//
