LOCUS       sequence-37940-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Kim ST, Xu B, Kastan MB
  TITLE     Involvement of the cohesin protein, Smc1, in Atm-dependent and 
            independent responses to DNA damage.
  JOURNAL   Genes Dev. 2002 Mar 1;16(5):560-70.
  PUBMED    11877376
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2002-03-1"; volume: "16(5)"; pages: "560-70"
COMMENT     Sequence Label: pCDNA3 5'cMyc-SMCl- S957A/S966A
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..135
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctccgc gggtttaaac ggatccgcca ccatggaaca gaaactcatc
      121 tctgaagagg atctggaatt catggggttc ctgaaactga ttgagattga gaactttaag
      181 tcgtacaagg gtcgacagat tatcggacca tttcagaggt tcaccgccat cattggaccc
      241 aatggctctg gtaagtcaaa tctcatggat gccatcagct ttgtgctagg tgaaaaaacc
      301 agcaacctgc gggtaaagac cctgcgggac ctgatccatg gagctcctgt gggcaagcca
      361 gctgccaacc gggcctttgt cagcatggtc tactctgagg agggtgctga ggaccgtacc
      421 tttgcccgtg tcattgtagg aggttcttct gagtacaaga tcaacaacaa agtggtccaa
      481 ctacatgagt acagtgagga attagagaag ttgggcattc tcatcaaagc tcgtaacttc
      541 ctcgttttcc agggtgctgt ggaatctatt gccatgaaga accccaaaga gaggacagct
      601 ctatttgaag agattagtcg ttctggggag ctggcgcagg agtatgacaa gcgaaagaag
      661 gaaatggtga aggctgaaga ggacacacag tttaattacc atcgcaagaa aaatattgcg
      721 gctgaacgca aggaagcaaa gcaggagaaa gaagaggctg accggtacca gcgcctgaag
      781 gatgaggtag tacgggctca ggtacagctg cagct
//
