LOCUS       sequence-379407-        6575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid for neuronal expression of soma-targeted 
            (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence 
            attaching the ribosomal tag to the jGCaMP8f protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-RiboL1-jGCaMP8f
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             431..448
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     730..751
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(857..878)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1118..1137)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2466..3054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2519..2539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2937..2948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3086..3562
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3285..3304)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3602..3742
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3602..3731
                     /label=AAV2 ITR
     rep_origin      3817..4272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3904..3923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4114..4135
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4289..4308)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4408..4430
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4468..4486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4554..4658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4659..4727,4728..5519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4877..4896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5690..6278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6179..6198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6340..6469
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        join(6522..6575,1..394)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
       61 atcagagagg gggaggggaa acaggatgcg gcgaggctcg tgcgcactgc cagcttcagc
      121 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
      181 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
      241 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
      301 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
      361 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aaccggctag aggatccacc
      421 cgccaccatg catcatcacc atcatcacac gcgtcgcaag aagaccttca aggaggtggc
      481 caccgccgtg aagatcatcg cccgcctgat gggcctgaag atcaacgtct atatcaaggc
      541 cgacaagcag aagaacggca tcaaggcgaa cttccacatc cgccacaaca tcgaggacgg
      601 cggcgtgcag ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      661 gctgcccgac aaccactacc tgagcgtgga gtccaaactt tcgaaagacc ccaacgagaa
      721 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      781 cgagctgtac aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac
      841 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      901 gtccggcgag ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      961 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1021 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1081 cgaaggctac atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1141 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1201 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacctgc cggaccaact
     1261 gactgaagag cagatcgcag aatttaaaga ggctttctcc ctatttgaca aggacgggga
     1321 tgggacaata acaaccaagg agctggggac ggtgatgcgg tctctcggac tgaaccccac
     1381 agaagcagag ctgcaggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcga
     1441 cttccctgag ttcctgacaa tgatggcaag aaaaatgaaa tacagggaca cggaagaaga
     1501 aattagagaa gcgttcggtg tgtttgataa ggatggcaat ggctacatca gtgcagcaga
     1561 gcttcgccac gtgatgacaa accttggaga gaagttaaca gatgaagagg ttgatgaaat
     1621 gatcagggaa gcagacatcg atggggatgg tcaggtaaac tacgaagagt ttgtacaaat
     1681 gatgacagcg aaggggggca gcggtgggtc tggagggact gggggtagcg gtggtagcgg
     1741 tgggacaggg ggaagcggcg gtagtggtgg aacaagcagc aaagtctcac gcgacaccct
     1801 gtacgaggcg gtgcgggaag tcctgcacgg gaaccagcgc aagcgccgca agtttctgga
     1861 gacggtggag ctgcagatca gcctgaagaa ctacgaccct cagaaggaca aacgtttctc
     1921 gggcaccgtc aggctcaagt ccaccccacg ccccaagttc tcggtgtgcg ttctggggga
     1981 ccagcagcac tgtgatgaag ccaaggccgt ggatatcccc cacatggaca tcgaggcgct
     2041 caagaaactt aacaaaaaca agaagttggt caagaagctg gctaagaagt acgatgcctt
     2101 tttggcctct gagtctctga ttaagcagat cccacgtatc ctgggcccag gcctaaacaa
     2161 ggctggcaag ttcccctccc tgctgacaca caatgaaaac atggtggcca aagtggatga
     2221 ggtgaaatcg acaatcaagt tccagatgaa gaaggtgctg tgtttggccg tcgctgttgg
     2281 ccacgtgaag atgaccgatg atgagctagt ctacaacatt catctggctg tcaatttctt
     2341 ggtgtccttg cttaagaaaa actggcaaaa cgtgcgggct ctgtacatca agagcaccat
     2401 gggcaagccc cagcgtctgt attagggcgc gcccctgcag ggaattcgat atcaagctta
     2461 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     2881 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     2941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3001 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3061 accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     3121 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     3181 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     3241 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     3301 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     3361 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     3421 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     3481 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     3541 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     3601 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3661 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     3721 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3781 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     3841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     3901 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3961 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4021 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4081 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4141 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     4201 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4261 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4321 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4381 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4441 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4501 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4561 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4681 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4801 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4861 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4981 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5041 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5221 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6301 tacggttcct ggccttttgc tggccttttg ctcacatgtc ctgcaggcag ctgcgcgctc
     6361 gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
     6421 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg cggccgcacg
     6481 cgtttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg
     6541 accaggatga ggcggggtgg gggtgcctac ctgac
//
