LOCUS       sequence-379411-        4125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-TRPM7
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(46..73)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(165..251)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     300..322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     314..331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     315..331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    347..446
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1782..1881)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1898..1917)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1899..1917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1922..1938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1922..1938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2369..3160
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3253..3841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3742..3761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3995..4012
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
       61 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
      121 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
      181 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      241 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
      301 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
      361 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
      421 gccaactttg tacaaaaaag ttggcaccat gtcccagaaa tcctggatag aaagcacttt
      481 gaccaagagg gaatgtgtat atattatacc aagttccaag gaccctcaca gatgccttcc
      541 aggatgtcaa atttgtcagc aactcgtcag gtgtttttgt ggtcgcttgg tcaagcaaca
      601 tgcttgtttt actgcaagtc ttgccatgaa atactcagat gtgaaattgg gtgaccattt
      661 taatcaggca atagaagaat ggtctgtgga aaagcataca gaacagagcc caacggatgc
      721 ttatggagtc ataaattttc aagggggttc tcattcctac agagctaagt atgtgaggct
      781 atcatatgac accaaacctg aagtcattct gcaacttctg cttaaagaat ggcaaatgga
      841 gttacccaaa cttgttatct ctgtacatgg gggcatgcag aaatttgagc ttcacccacg
      901 aatcaagcag ttgcttggaa aaggtcttat taaagctgca gttacaactg gagcctggat
      961 tttaactgga ggagtaaaca caggtgtggc aaaacatgtt ggagatgccc tcaaagaaca
     1021 tgcttccaga tcatctcgaa agatttgcac tatcggaata gctccatggg gagtgattga
     1081 aaacagaaat gatcttgttg ggagagatgt ggttgctcct tatcaaacct tattgaaccc
     1141 cctgagcaaa ttgaatgttt tgaataatct gcattcccat ttcatattgg tggatgatgg
     1201 cactgttgga aagtatgggg cggaagtcag actgagaaga gaacttgaaa aaactattaa
     1261 tcagcaaaga attcatgcta ggattggcca gggtgtccct gtggtggcac ttatatttga
     1321 gggtgggcca aatgttatcc tcacagttct tgaatacctt caggaaagcc cccctgttcc
     1381 agtagttgtg tgtgaaggaa caggcagagc tgcagatctg ctagcgtata ttcataaaca
     1441 aacagaagaa ggagggaatc ttcctgatgc agcagagccc gatattattt ccactatcaa
     1501 aaaaacattt aactttggcc agaatgaagc acttcattta tttcaaacac tgatggagtg
     1561 catgaaaaga aaggagctta tcactgtttt ccatattggg tcagatgaac atcaagatat
     1621 agatgtagca atacttactg cactgctaaa agatctgaag aggaatgatt atacgcctga
     1681 taaaattata tttcctcagg atgagccttc agatttgaat cttcagcctg gaaattccac
     1741 caaagaatca gaatcaacta attctgttcg tctgatgtta ttgccaactt tcttgtacaa
     1801 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1861 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     1921 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     1981 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     2041 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     2101 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     2161 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     2221 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2281 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     2341 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     2401 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     2461 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     2521 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     2581 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     2641 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     2701 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     2761 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     2821 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     2881 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     2941 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3001 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     3061 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     3121 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc
     3181 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3901 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3961 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4021 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     4081 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgc
//
